Naphthalocyanine as a New Photothermal Actuator for Lipid-Based

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Naphthalocyanine as a New Photothermal Actuator for Lipid-Based Drug Delivery Systems Joanne D Du, Linda Hong, Angel Tan, and Ben J. Boyd J. Phys. Chem. B, Just Accepted Manuscript • DOI: 10.1021/acs.jpcb.7b12234 • Publication Date (Web): 09 Jan 2018 Downloaded from http://pubs.acs.org on January 15, 2018

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The Journal of Physical Chemistry

Naphthalocyanine as a New Photothermal Actuator for Lipid-Based Drug Delivery Systems Joanne D. Du, Linda Hong, Angel Tan and Ben J. Boyd* Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville, VIC 3052, Australia ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville, VIC 3052, Australia *Email: [email protected]; Tel. (+613) 99039112; Fax (+613) 99039583

Abstract

One approach to address the substantial global burden of ocular diseases such as aged related macular degeneration is using light-activated drug delivery to obviate the need for highly invasive and frequent need for costly intravitreal injections. To enable such systems, new light responsive materials are required. This communication reports the use of silicon 2,3naphthalocyanine bis(trihexylsilyloxide) (SiNC), a small molecule photosensitizer as a new actuator for triggering light responsive lipid-based drug delivery systems. Small angle X-ray scattering was used to confirm that the addition of SiNC imparted light sensitivity to the lipid

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systems, resulting in a complete phase transition within 20 s of near infrared irradiation. The phase transition was also reversible, suggesting potential for on-demand drug delivery. When compared to the phase transitions induced using alternative light responsive actuators, gold nanorods and graphene, there were some differences in phase behaviour. Namely, the phytantriol with SiNC system transitioned directly to the inverse micellar phase, skipping the intermediate inverse hexagonal structure. The photodynamic properties and efficiency in controlling the release of drug suggest that SiNC-actuated lipid systems have potential to reduce the burden of repeated intravitreal injections.

Introduction Age-related macular degeneration (AMD) is a chronic eye disease that requires bimonthly intravitreal injections to deliver inhibitors of the vascular endothelial growth factor (anti-VEGF) to slow the neovascularization that causes irreversible vision impairment and blindness in patients.1 The highly invasive, frequent and costly treatments are a substantial global burden, estimated to affect 196 million older people by 2020, increasing to 288 million by 2040.2 Considerable efforts have been directed towards reducing the burden of the current antiVEGF treatment.3-4 Lipid-based liquid crystalline nanostructures including the lamellar phase (Lα), bicontinuous cubic phase (V2), inverse hexagonal phase (H2) and inverse micellar phase


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(L2) are of increasing interest for ophthalmic delivery due to their biocompatibility and the ability to control drug release. Depending on the state of the aqueous channels being either open or closed, the release of encapsulated drugs can be either fast or slow, respectively.

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The

inverted phase structures are of particular interest in drug delivery applications due to their potential for encapsulation of hydrophilic compounds in the internalized aqueous compartments, and because of their thermodynamic stability to dilution, which together confer favourable controlled release properties. Recently, photo-sensitive ‘actuators’ have been incorporated into these structures to control the rate of drug release through manipulation of the nanostructure via an external light source.8-12 Materials that have a strong absorbance in the near-infrared (NIR) region are of particular interest as near infrared light can safely penetrate biological tissues due to its low absorption coefficient with water, lipids, and haemoglobin, and the penetration depth of up to 50 mm deep suggests potential for activation of systems implanted into the back of the eye. Several nanosized photothermal agents including gold nanoparticles13-15 and graphene derivatives16 have been explored as actuators as they can be tuned to absorb light in the NIR range and have been shown to efficiently convert the light into thermal energy to exhibit a change in the lipid structure. However, these particle-based actuators present problems with uncertainty about their location relative to the lipid material17-18 or aqueous phase16, and the potential for the nanosized agents to dissociate after administration, resulting in a reduction of the photothermal effect and limiting the practicality of these materials. Naphthalocyanine is a low molecular weight photosensitiser currently under investigation as a potential fluorescence imaging and phototherapeutic agent.19-23 It is hypothesized in this study that this small molecule photosensitizer will be embedded within the lipid structure to elicit


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a photothermal effect similar to other known nanosized agents, enabling activated release of encapsulated anti-VEGF therapy. This determines the potential use of SiNC as an actuator in two heat-sensitive lipid systems based on phytantriol (3,7,11,15-tetramethylhexadecane-1,2,3-triol)2426

and

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine

mixed

with

cholesterol

(POPE)27, that were previously investigated using particle-based photothermal agents. Phytantriol forms a bicontinuous cubic phase in excess water which transitions to an inverted hexagonal phase at elevated temperatures, while the POPE system transitions from a lamellar to an inverted hexagonal phase at elevated temperatures.

Materials and Methods Phytantriol (PHYT; 3,7,11,15-tetramethylhexadecane-1,2,3-triol) was purchased from DSM Nutritional Products (Kaiseraugst, Switzerland). 1-Palmitoyl-2-oleoyl-sn-glycero-3phosphoethanolamine (POPE) was purchased from Avanti Polar Lipids (Alabaster, USA). Silicon 2,3-naphthalocyanine bis(trihexylsilyloxide) (SiNC) and cholesterol were purchased from Sigma-Aldrich (St. Louis, USA). Lipid dispersions were prepared by dissolving PHYT with 5 mol% SiNC and POPE with 20 mol% cholesterol and 10 mol% SiNC in THF and the solution was then dried under a stream of nitrogen gas for 3 h, then under vacuum overnight at 50 °C to ensure complete removal of solvent. Blank lipid samples were prepared in the same manner without SiNC. The peak absorbance of SiNC in lipid systems was observed around 790 nm (Refer to S1). The lipid film was then hydrated in a 20:1 (w/w) ratio with PBS containing 1 w/w% Pluronic® F108 stabiliser at pH 7.4. The lipid dispersions were allowed to equilibrate for


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24 h before investigation using small angle X-ray scattering (SAXS) at the Australian Synchrotron to qualitatively identify the internal nanostructure formed in situ.28 The synchrotron X-ray beam was tuned to a wavelength of 0.954 Å (13.0 keV) at a camera to detector distance of 964.4 mm which gave the q-range of the scattering vector from 0.0176 to 1.016 Å-1. A silver behenate standard (d-spacing = 58.38 Å) was used for the q range calibration. For equilibrium measurement, samples were transferred into 1.5 mm diameter glass capillaries and the 2D SAXS patterns were acquired using a Pilatus 1M detector with active area 169 to 179 mm2 and with a pixel size of 172 µm. For the temperature-dependent results, the samples were placed in a temperature-controlled capillary holder, and heated from 25 to 80 °C at 5 °C intervals with equilibration for 10 min at each temperature prior to acquisition. The acquisition time was 1 s at 100% flux. In time-resolved measurements, a Class IIIB fiber coupled laser system (MDL-808) with the power output at 400 mW at 808 nm (Changchun New Industries, China) was mounted 5 cm from the sample at a tangential angle to the X-ray beam and samples in a temperature controlled capillary holder were allowed to equilibrate for 10 min at 37 °C before they were illuminated remotely by a computer control system. SAXS patterns were collected before, during and after laser activation. The acquisition time was 0.1 s at intervals of every 8-10 s at 20% flux for 2 min to avoid radiation damage from the synchrotron beam during data accumulation. The two dimensional scattering patterns were integrated into the one dimensional scattering function I(q) using the in-house developed software package, scatterBrain.29 Scattering curves are plotted as a function of relative intensity, I, versus q and phase structures were identified by indexing the Bragg peaks to known relative spacing ratios.3031

The lamellar phase (Lα) shows reflections at spacing ratios of 1:2:3:4, bicontinuous cubic

phase (V2) can be identified by the reflection spacing of √2: √3: √4:√6 (Pn3m), inverse


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hexagonal phase (H2) has a spacing ratio of 1: √3: √4 and inverse micellar phase (L2) is evident by a broad peak between 0.15 and 0.20 Å. The apparent temperature (Tapp) of the lipid system during irradiation was determined using a calibration plot of the lattice parameter vs. temperature using the temperature-dependent SAXS results, where the lattice parameter is a measure of the internal phase structure calculated from the relative peak positions. Results The effect of NIR irradiation on the nanostructure was evaluated using time-resolved small angle X-ray scattering (SAXS). After reduction to express the SAXS data as intensity versus magnitude of the scattering vector, the structures were identified by the spacing of the Bragg peaks and lattice parameters were calculated by the relative positions of the peaks. Figure 1 illustrates the time dependence of NIR irradiation on structure when SiNC is incorporated in the PHYT and POPE systems. The PHYT + SiNC system (Figure 1A) transitioned from the bicontinuous cubic phase (indicated by the indexed peak positions at √2, √3, √4, √6) to the inverse micellar phase (characterised by the broad hump at q~0.17 Å-1) with less than 10 s irradiation, and reverted back to the original cubic phase within 30 s of ceasing the irradiation. The POPE + SiNC (Figure 1C) system was less sensitive than the PHYT system, retaining the equilibrium Lα structure (indicated by peaks at spacing ratios 1:2:3) until 20 s of irradiation. The system converted to the H2 phase, indicated by the peaks at ratios 1: √3: √4. Approximately 40 s after ceasing the irradiation, the H2 transitioned back to the starting Lα structure. No change in structure was observed upon irradiation for lipid systems without SiNC, as evident in Figure 1B and D. .


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Figure 1. Effect of NIR irradiation on the structure of PHYT and POPE-based system maintained at 37 °C. A. PHYT + SiNC, B. Blank PHYT, C. POPE + SiNC and D. Blank POPE. The red curves indicate the time points exposed to NIR irradiation.


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To better understand the dynamic behaviour of these systems, and to determine any effect of the SiNC on self-assembly in the absence of NIR light, equilibrium temperature ramps were conducted in the presence of SiNC, where the SAXS patterns were acquired at fixed increasing temperatures, and the lattice parameter calculated for the phases present at each temperature. The equilibrium phase behaviour of the PHYT-based cubic phase in the presence of SiNC is seen in Figure 2A, where a phase transition from the V2 phase to the L2 phase occurred at approximately 55 °C. Previous temperature ramps studied in the absence of, and on inclusion of the hydrophobic gold nanorods17 and graphene nanosheets16 as the photothermal actuator showed an intermediate H2 phase between approximately 45 and 65 °C (Figure 2B and C). It is known that an increase in temperature induces the formation of more hydrophobic structures due to an increase in the effective volume of the hydrophobic tails.32 However, it is also known that the formation of the H2 phase is energetically disfavoured due to the requirement to fill geometric voids at the vertices where hydrophobic tails meet in the structure between the hexagonally packed water-filled rods.33 The transition directly to the L2 phase without transitioning through the disfavoured H2 structure has been observed previously for PHYT-based dispersions in the absence of hydrophobic additives,34 but not for bulk phase samples which was the format for the comparator gold nanorod and graphene containing systems. This suggests that SiNC does not act as a hydrophobic additive, essentially leaving the packing of the lipids unaffected until irradiated with NIR light.


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Figure 2. Temperature-dependent phase behaviour for PHYT system containing A. SiNC determined in this study, B. increasing concentration of gold nanorods17 and C. graphene nanosheets16. Reproduced with permission from American Chemical Society and the Royal Society of Chemistry.


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By utilising the lattice parameters calculated from the temperature-dependent equilibrium data in Figure 2 as a calibration curve,13 the effect of irradiation on the lipid systems can be converted to the apparent temperature (Tapp) of the lipid system at each acquisition time (Figure 3). The Tapp is a measure of disruption of lipid packing under the stimulus, and allows comparison of efficiency (magnitude of change in lipid packing) and effectiveness (required concentration) across different photoactuator systems. It should be noted that a global change in temperature cannot be detected on irradiation of these systems – the localised heating that causes changes in lipid packing is rapidly dissipated through the material. The magnitude of change in Tapp suggests that a much lower concentration of SiNC might be able to be used to stimulate the phase transition of both PHYT and POPE systems. The L2 peak for the PHYT + SiNC system at 60 s of irradiation corresponded to a Tapp of 80 °C (Figure 3A). Therefore, 60 s irradiation of the PHYT system with SiNC induced an increase of approximately 40 °C, which is similar to the previously studied PHYT + graphene system (Figure 3C). Similar to the PHYT + SiNC system, the POPE + SiNC system showed a rapid increase in Tapp of 40 °C within 30 s of irradiation (Figure 3B). The actuation by SiNC in this case was more effective than inclusion of gold nanorods at 3 nM in past work, which reached a maximum increase of 25 °C with 30 s of irradiation (Figure 3D). SiNC is proposed to provide a greater photothermal effect as its photothermal effect can more readily affect the lipid molecules as it is embedded within the lipid structure, whereas the other actuators would suffer some dissipation of heat through the aqueous regions and as a result, be overall less effective actuators.


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Figure 3. Effect of NIR irradiation on the apparent temperature (Tapp) for the PHYT and POPE systems. A. PHYT + SiNC (open circle = blank PHYT), B. POPE + SiNC (open circle = blank POPE), C. PHYT + graphene16 (X = blank PHYT) and D. POPE + gold nanorods15 (closed circle = blank POPE). Reproduced with permission from The Royal Society of Chemistry and the PCCP Owner Societies. Conclusion In conclusion, the molecular actuator, SiNC, was shown for the first time to impart photothermal sensitivity to lipid systems. The SiNC-containing lipid systems required a shorter irradiation time to elicit a complete phase transition than the equivalent systems containing gold


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nanorods or graphene. The lipids studied in this communication were selected because they are established platforms known to be responsive to other photothermal stimuli. It is recognised however that their phase behaviour will result in a release profile that is not ideal for on-demand drug release application, as they tend to transition to the slow-releasing inverse hexagonal and inverse micellar phase structures. An ideal release profile would be a lipid system that can transition from the Lα to V2 phase to exhibit a slow to fast release profile with an increase in temperature/NIR stimulus, and such lipid systems are currently under investigation .

Author Information The authors declare no competing financial interests. Acknowledgements BJB is the recipient of an Australian Research Council Future Fellowship. This research was undertaken on the SAXS/WAXS beamline at the Australian Synchrotron, VIC. JDD is supported through an Australian Government Research Training Program Scholarship. These experiments were conducted on the SAXS/WAXS beamline at the Australian Synchrotron. The research was supported by the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology.

Supporting Information for Publication Figure S1 Absorbance spectrum of SiNC

References 1. Ohr, M.; Kaiser, P. K., Intravitreal Aflibercept Injection for Neovascular (Wet) AgeRelated Macular Degeneration. Exp. Opin. Pharmacother. 2012, 13, 585-591.


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Page 13 of 15 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60


2. Wong, W. L.; Su, X.; Li, X.; Cheung, C. M. G.; Klein, R.; Cheng, C.-Y.; Wong, T. Y., Global Prevalence of Age-Related Macular Degeneration and Disease Burden Projection for 2020 and 2040: a Systematic Review and Meta-analysis. Lancet 2014, 2, 106-116. 3. Ishikawa, M.; Jin, D.; Sawada, Y.; Abe, S.; Yoshitomi, T., Future Therapies of Wet AgeRelated Macular Degeneration. J. Ophthalmol. 2015, 2015, 138070. 4. Holz, F. G.; Schmitz-Valckenberg, S.; Fleckenstein, M., Recent Developments in the Treatment of Age-Related Macular Degeneration. J. Clin. Invest. 2014, 124, 1430-1438. 5. Lajunen, T.; Nurmi, R.; Kontturi, L.; Viitala, L.; Yliperttula, M.; Murtomäki, L.; Urtti, A., Light Activated Liposomes: Functionality and Prospects in Ocular Drug Delivery. J. Control. Release 2016, 244, Part B, 157-166. 6. Delplace, V.; Payne, S.; Shoichet, M., Delivery Strategies for Treatment of Age-Related Ocular Diseases: from a Biological Understanding to Biomaterial Solutions. J. Control. Release 2015, 219, 652-668. 7. Du, J. D.; Fong, W.-K.; Caliph, S.; Boyd, B. J., Lipid-Based Drug Delivery Systems in the Treatment of Wet Age-Related Macular Degeneration. Drug Deliv. Transl. Res. 2016, 6, 781-792. 8. Li, H.; Yang, X.; Zhou, Z.; Wang, K.; Li, C.; Qiao, H.; Oupicky, D.; Sun, M., NearInfrared Light-Triggered Drug Release from a Multiple Lipid Carrier Complex using an All-inOne Strategy. J. Control. Release 2017, 261, 126-137. 9. Eastoe, J.; Vesperinas, A., Self-Assembly of Light-Sensitive Surfactants. Soft Matter 2005, 1, 338-347. 10. Miranda, D.; Lovell, J. F., Mechanisms of Light-induced Liposome Permeabilization. Bioeng. Transl. Med. 2016, 1, 267-276. 11. Rwei, A. Y.; Zhan, C.; Wang, B.; Kohane, D. S., Multiply Repeatable and Adjustable On-Demand Phototriggered Local Anesthesia. J. Control. Release 2017, 251, 68-74. 12. Carter, K. A.; Shao, S.; Hoopes, M. I.; Luo, D.; Ahsan, B.; Grigoryants, V. M.; Song, W.; Huang, H.; Zhang, G.; Pandey, R. K., Porphyrin–Phospholipid Liposomes Permeabilized by Near-Infrared Light. Nat. Commun. 2014, 5, 3546. 13. Fong, W.-K.; Hanley, T. L.; Thierry, B.; Kirby, N.; Waddington, L. J.; Boyd, B. J., Controlling the Nanostructure of Gold Nanorod–Lyotropic Liquid-Crystalline Hybrid Materials using Near-Infrared Laser Irradiation. Langmuir 2012, 28, 14450-14460. 14. Kwon, H. J.; Byeon, Y.; Jeon, H. N.; Cho, S. H.; Han, H. D.; Shin, B. C., Gold ClusterLabeled Thermosensitive Liposmes Enhance Triggered Drug Release in the Tumor Microenvironment by a Photothermal Effect. J. Control. Release 2015, 216, 132-139. 15. Du, J. D.; Fong, W.-K.; Salentinig, S.; Caliph, S. M.; Hawley, A.; Boyd, B. J., Phospholipid-Based Self-Assembled Mesophase Systems for Light-Activated Drug Delivery. Phys. Chem. Chem. Phys. 2015, 17, 14021-14027. 16. Quinn, M. D. J.; Wang, T.; Du, J. D.; Boyd, B. J.; Hawley, A.; Notley, S. M., Graphene as a Photothermal Actuator for Control of Lipid Mesophase Structure. Nanoscale 2017, 9, 341348. 17. Fong, W.-K.; Hanley, T. L.; Thierry, B.; Kirby, N.; Boyd, B. J., Plasmonic Nanorods Provide Reversible Control over Nanostructure of Self-Assembled Drug Delivery Materials. Langmuir 2010, 26, 6136-6139. 18. Lee, J. H.; Shin, Y.; Lee, W.; Whang, K.; Kim, D.; Lee, L. P.; Choi, J. W.; Kang, T., General and Programmable Synthesis of Hybrid Liposome/Metal Nanoparticles. Sci. Adv. 2016, 2, e1601838.


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Page 14 of 15

19. Taratula, O.; Schumann, C.; Duong, T.; Taylor, K. L.; Taratula, O., DendrimerEncapsulated Naphthalocyanine as a Single Agent-Based Theranostic Nanoplatform for NearInfrared Fluorescence Imaging and Combinatorial Anticancer Phototherapy. Nanoscale 2015, 7, 3888-3902. 20. Taratula, O.; Doddapaneni, B. S.; Schumann, C.; Li, X.; Bracha, S.; Milovancev, M.; Alani, A. W. G.; Taratula, O., Naphthalocyanine-Based Biodegradable Polymeric Nanoparticles for Image-Guided Combinatorial Phototherapy. Chem. Mater. 2015, 27, 6155-6165. 21. Camerin, M.; Rello-Varona, S.; Villanueva, A.; Rodgers, M. A. J.; Jori, G., MetalloNaphthalocyanines as Photothermal Sensitisers for Experimental Tumours: In Vitro and In Vivo Studies. Lasers Surg. Med. 2009, 41, 665-673. 22. Zhang, Y.; Lovell, J. F., Recent Applications of Phthalocyanines and Naphthalocyanines for Imaging and Therapy. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2017, 9, e1420. 23. Zhang, Y.; Hong, H.; Sun, B.; Carter, K.; Qin, Y.; Wei, W.; Wang, D.; Jeon, M.; Geng, J.; Nickles, R. J., Surfactant-Stripped Naphthalocyanines for Multimodal Tumor Theranostics with Upconversion Guidance Cream. Nanoscale 2017, 9, 3391-3398. 24. Dong, Y.-D.; Dong, A. W.; Larson, I.; Rappolt, M.; Amenitsch, H.; Hanley, T.; Boyd, B. J., Impurities in Commercial Phytantriol Significantly alter its Lyotropic Liquid-Crystalline Phase Behavior. Langmuir 2008, 24, 6998-7003. 25. Mulet, X.; Boyd, B. J.; Drummond, C. J., Advances in Drug Delivery and Medical Imaging using Colloidal Lyotropic Liquid Crystalline Dispersions. J. Colloid Interface Sci. 2013, 393, 1-20. 26. Fong, W.-K.; Negrini, R.; Vallooran, J. J.; Mezzenga, R.; Boyd, B. J., Responsive SelfAssembled Nanostructured Lipid Systems for Drug Delivery and Diagnostics. J. Colloid Interface Sci. 2016, 484, 320-339. 27. Winter, R.; Erbes, J.; H. Templer, R.; M. Seddon, J.; Syrykh, A.; A. Warrender, N.; Rapp, G., Inverse Bicontinuous Cubic Phases in Fatty Acid/Phosphatidylcholine Mixtures: the Effects of Pressure and Lipid Composition. Phys. Chem. Chem. Phys. 1999, 1, 887-893. 28. Kirby, N. M.; Mudie, S. T.; Hawley, A. M.; Cookson, D. J.; Mertens, H. D. T.; Cowieson, N.; Samardzic-Boban, V., A Low-Background-Intensity Focusing Small-Angle XRay Scattering Undulator Beamline. J. Appl. Crystallogr. 2013, 46, 1670-1680. 29. ScatterBrain. 30. Boyd, B. J.; Dong, Y.-D.; Rades, T., Nonlamellar Liquid Crystalline Nanostructured Particles: Advances in Materials and Structure Determination. J. Liposome Res. 2009, 19, 12-28. 31. Hyde, S. T., Identification of Lyotropic Liquid Crystalline Mesophases. John Wiley & Sons, Ltd: 2001; p 299-332. 32. Israelachvili, J. N.; Mitchell, D. J.; Ninham, B. W., Theory of Self-Assembly of Hydrocarbon Amphiphiles into Micelles and Bilayers. J. Chem. Soc. Faraday Trans. 1976, 72, 1525-1568. 33. Rappolt, M.; Hickel, A.; Bringezu, F.; Lohner, K., Mechanism of the Lamellar/Inverse Hexagonal Phase Transition Examined by High Resolution X-Ray Diffraction. Biophys. J. 2003, 84, 3111-3122. 34. Dong, Y.-D.; Tilley, A. J.; Larson, I.; Lawrence, M. J.; Amenitsch, H.; Rappolt, M.; Hanley, T.; Boyd, B. J., Nonequilibrium Effects in Self-Assembled Mesophase Materials: Unexpected Supercooling Effects for Cubosomes and Hexosomes. Langmuir 2010, 26, 90009010.


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Naphthalocyanine as a New Photothermal Actuator for Lipid-Based

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