New Sesquiterpenes and C15 Acetogenins from the Marine Red Alga

Metabolites from the Sea Hare Aplysia fasciata. Efstathia Ioannou , Michela Nappo , Conxita Avila , Constantinos Vagias and Vassilios Roussis. Journal...
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Joumul of Natural Products V O ~54, . NO.4 , pp. 1025-1033, Jul-Aug 1991

I025

N E W SESQUITERPENES A N D CI5ACETOGENINS FROM THE MARINE RED ALGA LAURENCIA IMPLICATA ANTHONYD. WRIGHT,GABRIELE M. KONIG, and Orro STICHER

Department of Pharmacy, Swiss Federal Institute of Technology (ETH) Zurich, CH-8092 Zurich, Switzerland AssTRAcT.-From a single collection of the red alga Laurencta implicata fourteen secon5-11), one diterpene(12),and four C15dary metabolites, including nine sesquiterpenes (1,3, acetogenins (13-15, 17) have been isolated and characterized. Two of the sesquiterpenes, ( 1Rf,4R+,5Z,9Sf)-5-isopropyl-3,3,~-trimethylbicyclo[4. 3.O)nona-5-en-4-01 [l]and ( 1R+,4E, 9R*)-5-( 1 '-methykhan-1 '-01)-3,3,9-trimethylbicyclo[4.3.01nona-4-ene 131, and theCI5-acetogenins(1R+,4Rr,6Sf,7SX,9Rr,10Rr, 12Sr, 13R+)-4:7,6:12-bisepoxy-9chloro-l,lO, 13-tribromopentadeca-l,2dieneI131 and ( 1Rf,4R*,bS+,7S+,9R*,10R*, 12R+, 13Rf)-1, 10-dibromo-4:7,6:13,9:12-trisepoxypentadeca-1,2-diene [141, are new natural products. For compounds 5-9 and 11 unambiguous 'H- and "C-nmr assignments were made.

a*,

Algae of the genus Luurencia are very diverse with respect to their chemical constituents. Secondary metabolites from plants of this genus are known to include sesquiterpenes, diterpenes, triterpenes, and C15-acetogenins of many different structural types (1). In the course of our continuing studies of the secondary metabolite content of marine algae and their relationship to site and time of collection of the algae, we investigated a sample of Luurencia implicata]. Agardh (Rhodomelaceae), collected once again from Britomart Reef (2) (central region of The Great Barrier Reef), Queensland, Australia. RESULTS AND DISCUSSION The CH,CI, solubles from the freeze-dried algal tissue were chromatographed over Si gel (3). Selected fractions were then further purified by hplc according to Table 1 to afford fourteen pure compounds 1,3, 5-15, and 17. Compound 1 had a molecular formula of C,5H260. The occurrence of only two resonances in the 13C-nmr spectrum for sp2 hybridized carbons 1133.6 (s), 140.7 (s) ppm} dictated 1 to be a bicyclic molecule. Further, from the 13C-nmr and ir spectroscopic data 172.9 (d) ppm, 3470 cm-'7 i t was apparent that the oxygen within 1 was present in the form of a secondary hydroxyl function, which underwent smooth acetylation to give 2. The 'H-nmr data (C6D6) for 1 clearly showed the presence of two tertiary methyls {6 0.74 (s), 1.04 (s)} and three secondary methyl functions, two being part of an isopropyl moiety {6 0.97 ( d , J = 6 . 2 Hz), 1.02 ( d , J = 7 . 0 Hz), 1.14 ( d , J = 7 . 0 Hz), 2.57 (qq, J = 7 . 0 , 7.0 Hz)]. These data together with the information gained from the 'H-'H COSY spectrum of 1 and the results of one-bond ( J = 136 Hz) and long-range ( J = 10 Hz) 13C-'H correlation experiments allowed the structure of 1 to be deduced as that of a brasilenol[4} derivative (4). The proposed structure of 1 contained three chiral centers, the stereochemistries of which were deduced mainly from the results of 2D NOESY measurements made with 1 and 2. Thus, in 1 the presence of an nOe between the proton at C- 1 and the protons of the C- 14 methyl group, which further showed nOe to H-4, clearly position these groups on the same side of the molecule. Additionally, in the acetate 2 of 1,the observed nOe between the H-1 and the protons of the C- 15 methyl group position these two groups on the same side of the molecule. The proposed stereochemistry at C-4 is further supported by the 1.1 Hz W coupling between the H-4 and one of the protons at C-2. For such a coupling to be observed between these two protons, the C-4 proton must be "in plane" with one of the protons at C-2 and thus have the stereochemistry as shown in 1. Compound 1 is ( 1RX,4RX, 5Z,9Sx)-5-isopropyl-3, 3,9-trimethylbicyclol4.3.O}nona-5-en-4-ol.

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1 R=OH 2

3

R=OAc

4

7 R=H,,R,=H

9

8

r(" 10

15

12

11

Compound 3, an oil with molecular formula C,,H260, contained a single carboncarbon double bond 1130.9 (d), 143.7 (s) ppm] and a tertiary alcohol 173.3 (s) ppm, 3450 cm-'1 function; it was thus bicyclic. From its 'H-nrnr spestral data (C6D6)four signalsfortertiarymethylgroupscould bediscerned16 1.33 (s), l . 3 1 (s), 1.09(s), 1.07 (s)] as well as a doublet resonance for a secondary methyl funtion [6 1.05 (d, J = 6.5 Hz)]. Of the four methyl groups, two were part of a gem-dimethyl grouping, with the others forming a -C(Me),OH moiety. As all of these methyl groups showed nOe interactions with the olefinic proton 16 5.89 (br s)] it was concluded that the associated groupings must be on either side of it. Further from the 'H-'H COSY spectrum of 3 the following coupling patterns could be discerned. The olefinic proton at C-4 showed long-range coupling to one of the protons at C-2 16 1.05 (m)] and the proton at C-6 16 1.93 (m)]. The proton at C-6 showed couplings to the proton at C- 116 1.19 (m)] and to both of the protons at C-7 16 1.42 (m), 1.96 (m)]; these C-7 protons intercoupled, and both had couplings to the protons of the C-8 methylene group. The latter two protons

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Fraction number

3

6

9

10

11

12

14

15

16

Separation method'

A

B

C

C

D

E

F

F

F

Isolaterandtheiramounts(mg)

11 (3) 9 (10)

11 ( 5 )

6 (57)

6 (43)

1 (32) 8 (48) 17 ( 5 )

3 (5) 7 (46) 7 (9) 10 (31) 10 (17)

7 (75) 5 (150) 12 (8) 15 (8)

13 (73) 14 (26)

14* *Arrows indicate nOe's.

15

16

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^

E

v

c n N

P

v

v

N N

z-

v N n

[Vol. 54, No. 4

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also intercoupled and showed further coupling to the proton at C-9 16 1.44 (m)], which in turn coupled to the protons of the C- 15 methyl group and the proton at C- 1, which coupled to one of the protons at C-2 16 1.05 (m)]. These observations and the implied structural features together with 'H- and '3C-nmr data comparisons between 1 and 3 indicated the latter to be a structural isomer of 1. The nature of the positional isomerism was deduced from evaluation of inter-proton coupling patterns and additions of lanthanide shift reagent [Eu(fod),] to the nmr sample of 3. As a result of Eu(fod), additions the resonances for two methyl groups 16 1.3 1 (s) , 1.33 (s)], the single olefinic proton [6 5.49 (dd,J= 1.3, 2.4 Hz)], a methine proton [6 1.93 (m)], and the protons from the C-7 methylene group E6 1.42 (m), 1.96 (m)] all moved between 5.7 and 1 1. l ppm to higher frequencies; i. e. , they became more deshielded. From inter-proton coupling patterns it was also evident that one of the protons at C-2 must have a dihedral angle of approximately 90" with the proton at C- 1. The inter-proton coupling pattern observed for the C- 1 proton, in the 'H-nmr shifted spectrum of 3, revealed that this proton had large couplings (ca. 10 Hz) to the protons at C-6 and C-9 as well as a large coupling (12.9 Hz) and a small coupling (2.5 Hz) to the protons at C2. These observations and the results of the shift reagent study (see Experimental) indicate the C- 15 methyl group to be a and the proton at C-6 to be p. Compound 3 is ( l R L , 4E,6SX,9RX)-5-(l'-methylethan-l'-o1)-3,3,9-trimethylbicyclo~4.3.0~nona-4-ene. Compounds 5-11 were all sesquiterpenes that had been previously reported from Luurencia species. For compounds 8 (palisadin B) and 10 (palisol) all our spectroscopic data compared well with those reported ( 5 ) . For compounds 5 (aplysistatin) ( 6 ) ,6 (5phydroxyaplysistatin) (7), 7 (palisadin A) (5), 9 (€9,and 11 (9), the assignments of the H- and 13C-nmr resonances were either ambiguous, incomplete, or in some cases incorrect, while for 9 and 11 no 13C-nmr data.were available. Detailed nmr studies of compounds 5, 6, 7, 9, and 11 now allow us to provide these data in an unambiguous and complete form (Tables 2 and 3). For each of the compounds 5 , 6 , 7 , 9 ,and 11 the assignments were based on 2D 13C-lH one-bond ( J = 136 Hz) and long-range ( J = 10 Hz) (5 only) as well as 'H-'H correlation experiments (see Experimental). Compound 12 was identified as one of the diastereomers of trans-phytol (10). We report here (see Experimental) complete 'H- and 13C-nmr data for this compound.

'

T ~ L 3.E 13C-nrnr(75.5 MHZ, CDCI,)

'

Carbon

6

5

c-1 c-2 c-3 c-4 c-5 C-6 c-7 c-8 c-9 c-10 c-11 c-12 C-13 C-14 C-15

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

Data for

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

69.7 66.6 131.8 142.9 27.1 50.9 78.9 37.5 32.3 65.2 40.8 169.0 21.5 17.8 30.6

(t)

(d) (s)

(d) (t)

(d) (s) (t) (t)

(d) (s) (s)

(9) (9) (9)

69.8 66.2 133.6 141.9 67.8 54.7 79.2 39.1 32.3 65.7 41.6 169.5 25.2 19.1 30.7

5 , 6 , 7,9, and 11.

Compound

7 (t)

(d) (s)

(d) (d) (d) (s) (t) (t)

(d) (s) (5)

(9) (9)

(4

71.9 70.0 141.8 121.0 26.2 51.7 77.9 31.5 32.6 66.2 40.9 71.0 21.9 17.9 30.8

9 (t)

(d) (5)

(d) (t)

(d) (5)

(t) (t)

(d) (s) (t)

(9) (9) (9)

113.2 133.9 132.1 137.0 24.9 52.7 145.6 32.6 123.2 130.6 37.2 19.8 109.8 25.1 30.2

11 (t)

(d) (s)

(d) (t)

(d) (s) (t)

(d) (d) (s)

(9) (t)

(9) (9)

39.5 63.1 71. 1 40.4 31.6 47.8 139.7 123.0 36.3 60.9 42.8 24.7 26.0 17.2 24.2

(t)

(d) (s) (t) (t) (s) (5)

(d) (t)

(d) (s)

(9) (9) (9) (9)

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The final group of metabolites 13-17 to be discussed here are all C,, acetogenins. Compound 13 had the molecular formula C1,H2,Br3CIO2 by ms. From its ',C-nmr data (Table 4) the presence of a bromoallene moiety was suggested E20 1.6 (s), 10 1.8 (d), 7 4 . 1 (d) ppm] as were seven other carbons bearing electron withdrawing substituents; 13 was thus bicyclic. The oxygen functionalities within 13 were present as ethers since neither the ',C-nmr or ir spectroscopic data evidenced carbonyl or hydroxyl functionality. On the basis of the results from one-bond ',C-'H and 'H-'H correlation experiments a continuous chain of carbons from C-1 to C-15 could be discerned:

CHBr=C=CHCH(O)-CH2-CH(O)-CH(O)-CH2-CHCl-CHBr-CH2-CH(O)-CHBrCH2-CH3.The points of closure for the two ethers, between C-4 and C-7 and between C-6 and C-12, were made on the basis of 'H- and ',C-nmr data comparisons between 13, 15, and 17 (Table 4). From these comparisons it was also clear that the C1 function must reside at C-9. Stereochemically 13 and 17 [structure established by X-ray (13)] were obviously very similar on the basis of their corresponding 'H- and l3C-nrnr data for most centers. The stereochemistry at C-9 was deduced from the results of a 2D NOESY measurement made with 13. Compound 13 is thus ( 1RX,4RX,6SX,7SX,9RX, 10RX,12SX,13RX)-4:7,6:12-bisepoxy-9-chloro-1,10,13-tribromopentadeca1,2diene. '3C-nmr (75.5 MHZ, CDCI,) Data for 13,14,15,and 17.

TABLE4.

Compound

Carbon

14

13 c-1 c-2 c-3 c-4 c-5 C-6 c-7 C-8

c-9 c-10 c-11 c-12 C-13 C-14 C-15

. . . . . . . . . . . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . .

. . . . . . . . . . .

. . . . . . . . . .

. . . . . . . . . .

. . . . . . . . . .

. . . . . . . . . .

. . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

74.1 201.6 101.8 74.1 39.3 81.5 78.4 37.3 59.5 58.6 40.6 79.0 60.2 27.2 12.3

(d) (s)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(q)

74.1 201.3 101.2 72.5 38.3 75.2 77.1 31.0 78.7 59.7 31.3 74.8 86.8 21.7 10.4

17

15 (d) (s)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(q)

73.9 201.4 102.1 74.5 39.0 72.7 79.6 26.7 127.2 129.3 34.7 52.8 84.4 23.1 11.4

(d) (5)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(9)

74.0 201.8 101.8 74.4 39.0 81.9 78.4 32.9 70.3 57.0 40.3 79.2 60.3 27.0 12.3 21.3 170.2

(s) (s) (d)

(d) (t)

(d) (d) (t)

(d) (d) (t)

(d) (d) (t)

(q) (9) (s)

Compound 14, had the molecular formula C1,H,,O3Br2 and was found to be a tricyclic molecule, a trisether. Again, as for compound 13, it was possible to establish linkages between all fifteen carbons on the basis of 2D 'H- 'H and 13C-'H correlation spectra obtained with 14 dissolved in either C6D6 or CDC1,: CHBr=C=CH-CH(0)CH2-CH(O)-CH(O)-CH2-CH(O)-CHBr-CH2-CH(O)-CH(O)-CH~-CH~. Once the C,, chain for 14 had been pieced together, the points of closure for the three ethers and the position of the bromine remained to be established. From comparisons of 13C-nmr data of 14 and l3,15,17(Table 4), it appeared that the ether linkages occur as a result of closures between C-4 and C-7, and C-6 with either C-12 or C-13, and C-9 with either C-12 or C-13, with the bromine residing at C-10. To resolve the last two points of closure for the ethers, a long range l3C-'H correlation experiment ( J = 7 Hz) was

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performed. The results of this experiment revealed a long-range coupling between H12 and C-9, clearly indicating the final two ether linkages to occur between C-9 and C12, and between C-6 and C-13. Once the basic framework for 14 was completed, the Dreiding model for 14 was constructed in such a way as to satisfy all of the inter-proton coupling information as well as the results obtained from a 2D NOESY measurement made with 14.The two most critical pieces of structural information for assigning the relative stereochemistry within 14 were the observations that one of the C- 11 protons (6 2.32 d, J = 11.5 Hz) had an almost 0 Hz coupling to both the protons at C- 10 and C- 12 as well as having an nOe interaction with the protons at C-6 and C-7, clearly defining the stereochemistry for a major part of the molecule. The centers not confirmed stereochemically from these observations (C-4 and C- 13) were assigned on the basis of I3C-nrnr data comparisons (Table 4 ) and the results of the aforementioned 2D NOESY measurement. Compound 14 is thus ( 1RX,4RX,6SX,7SX,9RX, 10RX,12RX,13RX)I,lO-dibromo-4:7,6: 13,9: 12-trisepoxypentadeca- 1,2-diene. Compound 15 proved to be the known metabolite neo-laurallene (11). Compound 17 was also a previously reported acetogenin (12). In the report for 17 (12), 13C- and 'H-nmr resonances were assigned on the basis of literature comparisons that have since proved to be unreliable; therefore a one-bond I3C-'H ( J = 136 Hz) correlation experiment was performed. As a direct result of this measurement and the resulting assignments, it was clear that the proposed structure 16 should be revised to 17,which has been recently reported as a semi-synthetic compound (13). Three previous chemical investigations of L. implicata from The Great Barrier Reef region of Australia yielded either a predominance of terpenes or C,, acetogenins (2, 12). A prior investigation of L . implicata from Britomart Reef (2) afforded only terpenoid metabolites. In the current investigation of L . implicata, once again from Britomart Reef, both C,, acetogenins and terpenes were clearly shown to co-occur in the same sample; they thus provide a further example of secondary metabolite variation within an algal species. EXPERIMENTAL GENERAL EXPERIMENTALPROCEDURES.-AII one-bond 13C-'H correlation experiments were performed with proton detection (Bruker invbtp) with delays optimized for]= 136 Hz. Long-range I3C-lH correlation experiments were performed with carbon detection (Bruker hxco) with delays optimized for either) = 7, 10, or 15 Hz. Remaining details are as in Konig et ai. (14). PLANTMATERIAL.-The algal material was obtained in September 1989, from Britomart Reef in the central region of The Great Barrier Reef, Australia. Plants growing at C-3 m depth were collected, deep frozen, and, on return to the laboratory, freeze-dried. A voucher specimen is deposited at the Department of Chemistry and Biochemistry, James Cook University, Townsville, Australia (voucher number BR891B). EXTRACTIONAND IsOLATION.-The dry algal tissue (370 g) was exhaustively extracted with 2 liters ofCH,CI, and then with an equivalent volume ofMeOH to afford 3.7 g (1%) ofCH,CI,-soluble material. Vacuum liquid chromatography (vlc) (3) of the crude extract over Si gel using hexane with increasing proportions of EtOAc as eluent afforded 16 fractions each ofapproximately 90 ml. TICand 'H-nmr investigation of these fractions indicated fractions 3, 5 , 9-12, and 14-16 to be of further interest. Separation of these fractions according to Table 1 yielded 14 pure compounds. ( 1R*,4Rf,5Z,9S+)-5-Isopropyl-3,3,9-trimethylbicyclo[4.3 .O)nona-5-en-4-01 El] was obtained as an equilibrium mixture of oil and solid at room temperature (32 mg, 0.009%): [a]**D -45.0°(CHCI,, r=0.03); ir v max (film) 3470, 2900, 1450, 1385, 1010,985 cm-'; 'H nmr(CDCI,, 300 MHz) 6 0.66 ( b r d , J = 7 . 5 H z , l H , O H ) , 0 . 7 8 ( ~3,H , H - l 3 ) , 1.01(d,]=7.OHz, 3 H , H - l l ) , 1.01(d,]=7.0Hz, 3H, H-12), 1.02 (s, 3H, H-14), 1.13 (d,]= 6.8 Hz, 3H, H-15), 1.14 (m, l H , H-2), 1.34 (m, l H , H9), 1.36(m, 1H,H-2), 1.63(brddd,)=5.6, 11.2, 11.4Hz, l H , H - l ) , 1 . 8 9 ( m , 2 H , H - 8 ) , 2 . 2 7 ( m , 2H, H-7), 2.63 (qq,J= 7.0, 7.0 Hz, l H , H-lo), 3.50 (br d,]= 7.5 Hz, H-4); 'H nmr (C6D6, 300 M H z ) S 0 . 7 4 ( ~3, H , H - 1 3 ) , 0 . 9 7 ( d , J = 6 . 2 H ~3H,H-15), , 1 . 0 2 ( d , J = 7 . 0 H ~ ,3 H , H - l l ) , 1 . 0 4 ( ~ , 3H, H-14), l.lO(m, l H , H-2), 1.10(m, l H , H-8), 1.14 (d,]=7.0 Hz, 3H, H-12), 1.25 (m, l H , H-

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9), 1.30(ddd,]= 1.1, 5.7, 12.6Hz, 1H,H-2), 1.54(ddd,J=5.7, 11.2, 11.2Hz, W H - U , 1.79(m, 1H,H-8),2.15(m,2H,H-7),2.57(qq,J=7.0,7.0Hz, lH,H-I0),3.45(brd,J=5.3Hz, 1H,H-4); '3Cnmr(CDC13, 75.5 MHz) 17.9(q, C-15), 21.3(q, C - l l ) , 21.8(q, C-12), 23.8(q, C-13), 26.6(t, C7), 27.4 (q, C-14), 30.3 (d, C-lo), 32.9 (t, C-8), 33.8 (t, C-2), 34.6 (s, C-3), 40.8(d, C-9), 47.0(d, CI), 72.9(d, C-4), 133.6 (s, C-G), 140.7 (s, C-5) ppm; 'H-13C nmr long rangecorrelations (CDCI,,]= 10 Hz)H-2(C-l, C-2, C-6), H-4(C-5, C-6), H-7 (C-5, C-6, C-7), H-ll(C-5, C-10, C - l l , C - l 2 ) , H-l2(C5 , C-10, C-11, C-12), H-13 (C-2, C-3, C-4, C-13, C-14), H-14(C-2, C-3, C-4, C-13, C-14), H-15 (C1, C-8, C-9, C- 15); elms m/z (rel. int.) [MI+ 222 (58), 207 (35), 205 (37), 204 (43), 193 (13), 189 (50), 180 (49), 179 (70), 167 (58), 166 (78), 123 (83), 43 (100); hrms 222.1913 (calcd for C15H260, 222.1985). ACETYLATIONOF COMPOUND 1.-To a CH,C12 solution of 1(9 mg in 2 ml), 500 ~1 of Ac20 was added, followed by 2 mg of dimethylaminopyridine. The resultant solution was stirred for 3 h at room temperature. At the end of this period the reaction was quenched with H 2 0 (5 ml), and the CH,CI, and H,O phases separated. The aqueous layer was further extracted with 2 X 5 ml portions of Et,O. The organic fractions were combined and the solvents removed in vacuo t o yield 8.7 mg ofmaterial that was purified by hplc [normal phase silica with ErOAc-hexane (1:99) as eluent) to yield 7.3 mg of pure 2. Compound 2 was obtained as an oil (7.3 mg): [U)22D - 133.5'(CHC13, c = O . 15); ir u max (film) 2950, 1735, 1365, 1235, 1015, 955 cm-'; 'H nmr (CDCI,, 300 MHz) 8 0.87 (s, 3H, H-13), 0.88 (s, 3H,H-14),0.91(d,]=6.9Hz,3H,H-ll),0.98(d,]=6.9Hz,3H,H-12), l.Ol(d,]=7.1Hz,3H, H-15), 1.19(m, 1H,H-2'), 1.21(m, 1H,H-8'), 1.36(ddd,J=1.2,5.6,12.4Hz, 1H,H-2),1.43(m, l H , H-9), 1.65(ddd,J=5.7, 11.6, 12.3Hz, l H , H - I ) , 1.91(m, lH,H-8),2.01(s,3H,H-17),2.31 (m, 2H, H-7), 2.57 (qq, 6.9, 6.9 Hz, l H , H-IO), 5.12 (s, l H , H-4); I3C-nmr(CDCI,, 75.5 MHz) 17.9 (q, C-15), 20.5 (q, C - l l ) , 21.0(q, C-12), 21.6(q, C-17), 23.6(q, C-13), 26.7 (q, C-14), 27.0(t, C-7), 30.3 (d, C-IO), 32.9 (t, C-8), 34.6 (s, C-3), 35.0 (t, C-2), 40.5 (d, C-9), 46.8 (d, 74.0 (d, C-4), 129.3(s, C-6), 143.4(s, C-5), 171.0(s, C-l6)ppm;eimsm/z(rel. int.)[M)+ 264(1), 222(1), 204(76), 189 (48), 166 (loo), 161 (47), 119 (32), 105 (28). ( 1R+,4E,&Sf,9R+)-5-(1'-Methylethan- 1'-01)-3,3,9-trimethylbicycl0[4.3.O)nona-4-ene [3] was isolatedasanoi1(5,0mg, 0.0015%): [U]"D -3.0°(CHCI,,r=0. 17);irvmax(film)3450,2920, 1460, 1375 cm-'; 'H nmr (C6D6, 300 MHz) 8 1.05 (m, 1H, H-2), 1.05 (d,]=6.5 Hz, 3H, H-15), 1.07 (s, 3H,H-14), 1.09(s, 3H,H-13), l . l 9 ( r n , I H , H - l ) , 1.31(s, 3H,H-11), 1.33(s,3H7H-12), 1.38(m, 1H, H-8), 1.42 (m, l H , H-7), 1.44 (m, IH, H-9), 1.67 ( d d d , j = 1.3, 2.2, 12.2 Hz, l H , H-2), 1.92 (m, 1H, H-8), 1.93 (m, IH, H-6), 1.96 (m, l H , H-7), 5.49 (dd,]= 1.3, 2.4 Hz, 1H, H-4); 'H nmr (C6D6,300MHz)withEu(fod),added8 1.99(d,J=6.6Hz, 3H, H-15), 2.43(ddd,]=6.1, 10.4, 10.8 Hz, l H , H-8), 2.46 (s, 3H, H-13), 2.49 (s, 3H, H-14), 3.26 (m, l H , H-9), 3.33 (m, 1H, €3-81, 3.44 ( d d , J = 2 . 5 , 12.2 Hz, l H , H-2), 3.62 (dd,]= 12.2, 12.9 Hz, l H , H-2), 4.54 (dddd,]=2.5, 9.8, 10.1, 12.9 Hz, IH, H- l), 7.13 (m, IH, H-7), 7.77 (m, l H , H-6), 7.85 (m, l H , H-7), 11.15 (br s, 3H, H-12), 12.41(brs, 3H, H - l l ) , 13.99(brs, l H , H-4); 'Hnmr(c6D6,300MHz)A6valuesfromEu(fod), shift reagentstudyH-1(3.34), H-2(1.77, 2.27), H-4(8.50), H-6(5.84), H-7 (5.71, 5.89), H-8(0.41, 1.41), H-9 (1.82). H-11 ( l l . l O ) , H-12 (9.82), H-13 (1.37), H-14 (1.42), H-15 (0.94); I3C nmr (CDCI,, 75.5 MHz) 18.9(q, C-15), 29.2 (t, C-7), 29.8 (q, C-14), 29.8 (9, C-lU, 29.9(q9 C - W 31.9 (q, C-13), 32.3 (t, C-8), 34.5 (s, C-3), 36.4 (d, C-9), 40.9 (t, C-2), 45.8(d, C-6), 49.4(d, C-l), 73.3 (s, C-lo), 130.9 (d, C-4), 143.7 (s, C-5) ppm; eims m/z (rel. int.) [MI+ 222 (lo), 207 (49), 204 (35), 189 (30), 164 (40), 149 (50), 133 (18), 107 ( 3 4 , 95 (31), 59 (80); hrms 222.1922 (calcd for CI5H260, 222.1985). Compound 1 2 was isolated as athick clear oil (8 mg, 0.002%): [aI2,D -7.7'(CHC13, c = 0.16); iru max (film) 3400, 2945, 1735, 890 cm-'; 'H nmr (CDCI,, 300 MHz) 8 0.95 (d,]=6.7 Hz, 3H), 0.95 (d,]=6.7Hz, 3H),0.96(d,]=6.4Hz, 3H), 1.10-1.62(m, 19H), 1.53(brs, 3H), 1.98(dd,]=7.4, 7.6 Hz, 2H), 4.03 (d,] = 6.7 Hz, 2H), 5.45 (br dd,] = 6.7, 6.7 Hz, IH); I3C nmr (CDCI,, 75.5 MHz) 16.1 (q), 19.9 (q), 20.0 (q), 22.8 (q), 22.9 (9). 25.0 (t), 25.2 (t), 25.5 (t), 28.3 ( 4 , 33.1 (d), 33.2 (d), 37.0 (t), 37.7 (t), 37.8 (t), 37.9 (t), 39.7 (t), 40.1 (t), 59.4 (0, 124.8 (d), 138.5 (s) ppm. ( 1R*,4R+,6Sf,7S+,9R+,10R+,12S+,13R+)-4:7,6:12-Bisepoxy-9-chloro- 1,10,13-tribromopentadeca-l,2-diene[13) wasaclearmobileoil(73 mg, 0.02%): [a]22~+115.90(CHC13, r=0.24);irv max(film)2930, 1435, 1060,755,655 cm-'; 'Hnmr(CDCI3, 300MHz)G 1.10(t,]=7.2 Hz, 3H, H15), 1.76(rn, lH,H-14'), 1.96(ddq,j=2.0,7.2,7.3Hz, lH,H-14), 2.09(m, lH,H-5'),2.25(ddd, ] = 2 . 1 , 6 , 8 , 13.5Hz, IH,H-5),2.49(m,2H,H-l1), 2.50(m, 1H,H-8'), 2.80(ddd,]=1.5,7.5, 16.3 Hz, 1H, H-8), 3.80 (m, IH, H-13), 3.82 (m, l H , H-12). 4.07 ( b r d , J = 8.0 Hz, 1H, H-7), 4.19 (m, 1H, H-6), 4.78 (br t,]=4.8 Hz, IH, H-lo), 4.84 (m, 1H, H-9), 4.85 (m, 1H. H-4L 5.46(dd, 1= 5.7, 5.8 Hz, l H , H-3), 6.11 (dd,] = 1.7, 5.7 Hz, IH, H-1); I3Cnmr (CDCI,, 75.5 MHz) see Table 4; eirns m/z(rel. int.) [MI+ 512, 5 10, 508, 506 ( 1 , 2, 3, 2), 475 (I), 473 (2), 471 (2), 433 (2), 427 (3), 393 (15), 392 (IO), 391 (71), 390 (14), 389 (loo), 388 (6), 387 (44). 345 (lo), 309 (8), 227 (17A 191

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Wright etal. : Sesquiterpenes and Acetogenins

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(19), 147 (26), 107 (38), 8 1 (71); hrms 505.8961 (calcd for C,,H2~9Br281Br35C102, 505.8684), hrms 386.948 1 (calcd for C,2H,,79Br235C102,386.9364). ( l R r ,4Rr ,6Sr,7Sf ,9R+,10R+,12Rf, 13R+)-l,lO-Dibromo-4:7,6: 13,9: 12-trisepoxypentadeca-1,2diene [14] was isolated as an oil (26 mg, 0.007%): -21.4” (CHCI,, c = O . 19); ir u max (film) 2900, 1190, 1150, 1065, 655 cm-’; ’H nmr (CDCI,, 300 MHz) 6 0.96 (dd,]=7.5, 8.4 Hz, 3H, H15), 1.75(m, 2H,H-14), 1.82(brdd,J=5.5, 14.7Hz, IH, H-8’), 1.96(dddd,]= 1.0, 3.8,9.4, 15.5 Hz, 1H,H-1lf),2.06(ddd,]=6.3,6.5, 12.7Hz, 1H,H-5‘), 2.22(ddd,]=3.9,7.8, 12.7Hz, l H , H-5), 2.32(d,]=15.5Hz, l H , H - l l ) , 2.68(ddd,]=1.8, 10.4, 14.7Hz, l H , H - 8 ) , 3 . 7 2 ( d d d , J = 2.2, 7.0, 9.1 Hz, IH, H-13), 4.09 (dd,J= 2.2, 3.8 Hz, l H , H-12), 4.27 (m, l H , H-6), 4.29 (m, IH, H-9), 4.30 (m, IH, H-IO), 4.58 (ddd,]= 1.2, 6.6, 10.4 Hz, IH, H-7), 4.65 (m, IH, H-4), 5.39 (dd,] = 5.4, 5.7 HZ, l H , H-3), 6.11 (dd,] = 2.0, 5.7 Hz, IH, H- 1); ‘H nmr (C,D,, 300 MHz) 6 0.96 (dd,]=7.4, 7.4Hz, 3H,H-15), 1.40(ddd,]=3.7,9.3, 15.6H2, l H , H - l l ’ ) , 1.81(m,2H,H-14), 1.86(m,2H,H-5), 1.95(m, 1H,H-8’), 2.07(brd,/=15.6Hz, lH,H-l1),2.76(ddd,]=1.8, 10.4, 14.7 Hz, IH, H-8), 3.34 (ddd,]=2.0, 7.0, 7.1 Hz, l H , H-13), 3.57 (m, l H , H-12), 3.98 (dd, ]= 6.7, 14.0 Hz, IH, H-6), 4.18 (m, IH, H-IO), 4.20 (m, l H , H-9), 4.39 (m, IH, H-4), 4.67 (ddd, ] = t . O , 6 . 6 , 1 0 . 3 H ~ , IH,H-7),5.06(dd,]=5.1,5.5H~, lH,H-3),5.67(dd,]=2.1,5.7Hz,lH, H-1); ‘3CnmrseeTable4;eimsm/z(rel. int.){M]+410,408,406(1, 2, 2), 391(1), 389(1), 364(1), 362 (l), 329 (2), 327 (2), 247 (22), 245 (65), 209 (7), 191 (IO), 153 (9), 97 ( 2 4 , 8 1 (33L 57 (100); hrms 329.0595 (calcd for C lsH,,81Br03, 329.0576). ACKNOWLEDGMENTS We thank Mr. Rocky de Nys, Department ofchemistry and Biochemistry, James Cook University of North Queensland, Australia, for collection of the algal material, and Dr. Ian Price, Department of Botany, James Cook University of North Queensland, for species assignment. Dr. W . Fenical kindly provided original ‘H nmr and ir spectra for 11.Mass spectral data were provided by the Mass Spectral Service of the ETH Chemistry Department. LITERATURE CITED 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.

14.

K.L. Erickson, in: “Marine Natural Products.” Ed. by P. Scheuer, Academic Press, New York, 1983, Vol. V, p. 131. A.D. Wright, J.C. Coll, and I. R. Price, ]. Nut. Prod., 53, 845 (1990). J.C. Coll and B.F. Bowden,]. Nut. Prod., 49, 934 (1986). M.O. Stallard, W. Fenical, and J.S. Kittredge, Tetrahedron, 34, 2077 (1978). V.J. Paul and W . Fenical, Tetrahedron Lett., 21, 2787 (1980). G.R. Pettit, C.L. Herald, M.S. Allen, R.B. Von Dreele, L.D. Vanell, J.P.Y. Kao, and W. Blake, J . Am. Chem. Soc., 99,262 (1977). R. Capon, E.L. Ghisalberti, P.R. Jefferies, B.W. Skelton, andA.H. White, Tetrahedron, 37, 1613 (198 1). D.J. Faulkner, Nut. Prod. Rep., 1,25 1 (1984). B.M. Howard and W . Fenical, Tetrahedron Lett., 1687 (1975). A.R. Pinder, “The Chemistry ofTerpenes,” Chapman and Hall, London, 1960, p. 147. M. Suzuki, E. Kurosawa, A. Furusaki, S. Katsuragi, and T. Matsumoto, Chem. Lert., 1033 (1984). J.C. Coll and A.D. Wright, Aust. ]. Chem., 42,1685 (1989). M. Suzuki, Y.Sasage, M. Ikura, K. Hikichi, and E. Kurosawa, Phytochemistry, 28, 2145 (1989). G.M. Konig, A.D. Wright, and 0. Sticher,]. Nut. Prod., 53, 16 15 (1990).

Received 28 November 1990