novel central 5-hydroxytryptamine-receptor agonists - American

J.Med. Chem. 1988,31,92-99

92

N,N-Dialkylated Monophenolic trans -2-Phenylcyclopropylamines: Novel Central 5-Hydroxytryptamine Receptor Agonists Lars-Erik Arvidsson,*t Anette M. Johansson,? Uli Hacksell,? J. Lars G. Nilsson,? Kjell Svensson,*Stephan Hjorth,i Tor Magnusson,f Arvid Carlsson,t Per Lindberg,§ Bengt Andersson,s Doming0 Sanchez,§ Hiikan Wikstrom,§ and Staffan Sundell11 Department of Organic Pharmaceutical Chemistry, Uppsala Biomedical Center, University of Uppsala, S-751 23 Uppsala, Sweden, a n d Department of Pharmacology, Organic Chemistry Unit, Department of Pharmacology, a n d Department of Structural Chemistry, University of Goteborg, S-400 33 Goteborg, Sweden. Received May 11, 1987 N,N-Dialkylated monophenolic derivatives of trans-2-phenylcyclopropylamine were synthesized and tested for central 5-hydroxytryptamine (5-HT) and dopamine (DA) receptor stimulating activity by use of a biochemical test method in rats. A hydroxy substituent in the 2- or 3-position of the phenyl ring was required for 5-HT-receptor stimulation. N,N-Diethyl or N,N-di-n-propyl substitution gave the most potent 5-HT-receptor agonists. The 4-hydroxy and were inactive at central DA and 5-HT 3,4-dihydroxy derivatives of trans-2-phenyl-N~-di-n-propylcyclopropylamine receptors. In contrast, the corresponding 3-hydroxy derivative 18 and some of its derivatives weakly affected both DA and N E synthesis. Two of the most potent 5-HT-receptor agonists, trans-2-(2-hydroxyphenyl)-N,N-di-npropylcyclopropylamine (8) and the 3-hydroxy isomer 18 were resolved into the enantiomers. The 1R,2S enantiomers of 8 and 18 displayed 5-HT activity, while the 1S,2R enantiomers were inactive. Compound (1R,2S)-18, but not (1R,2S)-8, weakly affected rat brain DA and N E synthesis.

Scheme 1" In 1948 Burger and Yostl reported the synthesis of trans-2-phenylcyclopropylamine(29; tranylcypromine). Compound 29 was found to be an effective inhibitor of path aw , ba y I ' O monoamine oxidase (MAO), and Burger and co-workers ~4 W O / eC H , performed extensive structure-activity relationship (SAR) 6 studies of 2-phenylcyclopropylamines.2 Later studies 3 3 a : RO = 2 - O C H 3 suggested that the semirigid dopamine (DA) analogue 30 b: RO = 3-OCH3 exhibits adrenoceptor-stimulating properties but seems to lack DA a ~ t i v i t y .Compounds ~~~ 315s6and 327 are cyclopropyl analogues of the hallucinogenss 1-(2,5-dimethoxyA ..A RO 4-methylphenyl)-2-propylamine(DOM) and mescaline, respectively. The pharmacological actions of 31 and 32 have been described as LSD- or me~caline-like.~*~~J~

w

H

1

d pathway111

R3 R2+.4NH'

p

\C

H3

KP

RI

-

3

"Reagents: a = CH2N2,cat. Pd(OA&; b = 50% NaOH, MeOH; c = diphenylphosphoryl azide, tert-BuOH; d = aqueous HCHO, NaCNBH,; e = RNX, K2C03.

29: R l : R 2 = R 3 : R 4 = H 30' R 1 = R 2 = H;

R 3 = R 4 = OH

I

31:

Rl=R4=OCH3: Rz=

32: R 1 - H;

*

H;

R 3 z CH3

Rz= R3= R4=OCH3

To our knowledge, no monophenolic derivatives of 29 have been previously reported. In the present paper, we describe the synthesis and the central pharmacological effects of some monophenolic N,N-dialkylated derivatives of 29 and of the N,N-di-n-propyl derivative of the DA analogue 30. The novel compounds were tested for central monoaminergic activity in rats by use of a previously deof the scribed biochemical screening m e t h ~ d . Several ~ compounds were found to be centrally active 5-hydroxytryptamine (5-HT) receptor agonists. Two of the most potent 5-HT-receptoragonists, 8 and 18, were resolved into the enantiomers, and their absolute configurations were determined. Chemistry Cyclopropanes are usually synthesized by using olefins 'University of Uppsala. t Department of Pharmacology, University of Goteborg. 8 Organic Chemistry Unit, Department of Pharmacology, University of Goteborg. 11 Department of Structural Chemistry, University of Goteborg. 0022-2623/88/1831-0092$01.50/0

as starting materials.'O This is reflected in previous syntheses of trans-2-phenylcyclopropylamines,where the (1) Burger, A.; Yost, W. L. J. Am. Chem. SOC. 1948, 70, 2198. (2) For a review on SAR and pharmacology of MA0 inhibitors,

see: Kaiser, C.; Setler, P. E. In Burger's Medicinal Chemistry, 4th ed.; Wolff, M. E., Ed.; Wiley: New York, 1981; Part 111, p 997. The enantiomers of trans-2-phenylcyclopropylamine have been reviewed with reference to their pharmacological actions. Smith, D. F. Pharmakopsychiatr./Neuro-Psychopharmakol. 1980,13,130. (3) (a) Borgman, R. J.; Erhardt, P. W.; Gorczynski,R. J.; Anderson, W. G. J. Pharm. Pharrnacol. 1978,30,193.(b) Erhardt, P. W.; Gorczynski, R. J.; Anderson, W. G. J. Med. Chem. 1979, 22,907.(c) Gorczynski, R. J.; Anderson, W. G.; Erhardt, P. W.; Stout, D. M. J. Pharmacol. Exp. Ther. 1979,210,252. (4) (a) Additional DA-inactive derivatives of trans-2-phenylcyclopropylamine have been reported: Rehse, K.; Behncke, S.; Siemann, U.; Kehr, W. Arch. Pharm. (Weinheim, Ger.) 1980, 313, 221. (b) trans-2-(3,4-Methylenedioxyphenyl)cyclopropylamine has been reported to exhibit central DA stimulating activity after intracerebral administration, but conclusive evidence for a direct DA-receptor stimulation was not given; see: Costall, B.; Naylor, R. J.; Pinder, R. M. J . Pharm. Pharrnacol. 1974,26, 753. 0 1987 American Chemical Society

Journal of Medicinal Chemistry, 1988, Vol. 31, No. 1 93

Novel Central 5-Hydroxytryptamine Receptor Agonists

Table I. IH NMR Spectroscopic Dataa of trans-2-Phenylcyclopropylamine Derivatives

coupling constants, Hz chemical shifts, ppm OCH3 no. position 6, 6, 6d Jab J,C Jad Jbc Jbd Jcd 2 2-OCH3 2.52 2.75 1.35 1.31 3.5 10.1 6.8 4.4 7.8 -6.6 10.1 6.7 4.4 7.8 -6.7 12 3-OCH3 2.35 2.82 1.40 1.30 3.6 22 4-OCHa 2.27 2.71 1.32 1.21 3.7 10.0 6.6 4.3 7.8 -6.6 26 3,4-(0dH3)2 2.33 2.78 1.36 1.28 3.5 10.0 6.8 4.4 7.9 -6.7 “The sDectra of the hydrochlorides in CD,OD were recorded at 200 MHz (12. 22. and 26) or at 400 MHz (2) with tetramethvlsilane as internal reference. Assignments were madein analogy with a previously reported analysis of the 270-MHz lH NMR spectrum of transN,N-dimethyl-2-phenylcyclopropylamine (ref 5b). Coupling constants and chemical shift values were refined by spin-spin simulation using a JEOL FASNO 5 NMR spectrum simulation program.

intermediate trans-2-phenylcyclopropanecarboxylicacidsll have been formed by the following methods: (A) cyclopropanation of styrene derivatives with ethyl diazoacetate,3bJ2(B)Simmons-Smith reaction of trans-cinnamic (5) (a) (&)-31 and analogues: Aldous, F. A. B.; Barrass, B. C.; Brewster, K.; Buxton, D. A.; Green, D. M.; Pinder, R. M.; Rich, P.; Skeels, M.; Tutt, K. J. J. Med. Chem. 1974,17, 1100. (b) Pharmacology and SAR of (+)- and (-)-3l: Nichols, D. E.; Pfister, W. R.; Yim, G. K. W. Life Sci. 1978, 22, 2165. (c) Synthesis, absolute configuration, and pharmacology of (t)and (-)-31: Nichols, D. E.; Woodard, R.; Hathaway, B. A,; Lowy, M. T.; Yim, G. K. W. J. Med. Chem. 1979,22,458. (d) Conformational analysis of 31: Weintraub, H. J. R.; Nichols, D. E. Znt. J. Quantum Chem., Quantum Biol. Symp. 1978,No. 5,321. (6) Recently, two cyclopropyl ring methylated homologues of 31 have been reported; see: Jacob, J. N.; Nichols, D. E. J. Med. Chem. 1982,25, 526. (7) (a) Synthesis: Cooper, P. D. Can. J. Chem. 1970,48,3882. (b) Pharmacology: Walters, G. C.; Cooper, P. D. Nature (London) 1968,218,298. Copper, P. D.; Walters, G. C. Nature (London) 1972, 238, 96. (8) SAR of hallucinogens have recently been reviewed; see: (a) Nichols, D. E. J . Pharm. Sci. 1981, 70,839. (b) Gupta, S. P.; Singh, P.; Bindal, M. C. Chem. Rev. 1983,83,633. (c) Nichols, D. E.; Glennon, R. A. In Hallucinogens: Neurochemical, Behavioral and Clinical Perspectives; Jacobs, B. L., Ed.; Raven: New York, 1984; p 95. (9) For discussionsof the experimental design and the underlying concept, see, for example: (a) Wikstrom, H.; Lindberg, P.; Martinsson, P.; Hjorth, S.; Carlsson, A.; Hacksell,U.; Svensson, U.; Nilsson, J. L. G. J. Med. Chem. 1978,21,864. (b) AndBn, N.-E.; Carlsson, A.; Haggendal,J. Annu. Rev. Pharmacol. 1969, 9, 119. (c) Neff, N. H.; Neckers, L. M. Adu. Exp. Med. Biol. 1981, 133, 445. (10) For reviews on the reactions and the synthesis of cyclopropanes, see: (a) Wendisch, D. In Methoden der Organischen Chemie (Houben-Weyl), 4th ed.; Muller, E., Ed.; Georg Thieme: Stuttgart, 1971; Vol. IV, Part 3, p 15. (b) Boyle, P. H. In Rodd‘s Chemistry of Carbon Compounds, 2nd ed.; Ansell, M. F., Ed.; Elsevier: Amsterdam, 1974; Vol. IIA, Suppl., p 9 and also Vol. IIA of this series. (c) Halton, B. Alicyclic Chem. 1978, 6, 1 and the earlier volumes of this series. (d) Cooper, K. Gen. Synth. Methods 1980,3, 227 and the earlier volumes of this series. (e) Simmons, H. E.; Cairns, T. L.; Vladuchick, S. A.; Hoiness, C. M. Org. React. (N.Y.)1973,20, 1. (f) Bestmann, H. J.; Schmid, G.; Kisielowski, L. Zsr. J. Chem. 1982,22, 45. (11) Derivatives of trans-2-phenylcyclopropylamine have also been synthesized by other methods than through trans-2-phenylcyclopropanecarboxylic acids: (a) By palladium(I1) acetate catalyzed cyclopropanation of appropriate enamines with a large excess of diazomethane. The reported yields were low (10%); see ref 4a. (b) By an addition-elimination reaction of cinnamyl phenyl ether with lithium dialkylamides; see: Larcheveque, M.; Guillaumet, G.; Cuvigny, T.; CaubBre, P. Bull. Soc. Chim. Fr. 1975, 2275.

A

B

Figure 1. Atom numbering scheme and solid state (X-ray) conformation of (lR,2S)-(-)-8*HBr (A) and (lR,2S)-(-)-l&HBr

(B).

esters,loeand (C) reaction of tert-butyl cinnamate derivatives with dimethylsulfoxonium methylideels Method A has been reported to give mixtures of cis- and trans-2phenylcyclopropanecarboxylica c i d ~ , 3 ~and J ~ Jthe ~ use of method B has resulted in widely varying yields.loe Therefore, preliminary experiments were performed by means of method C. The preparation of the tert-butyl cinnamates, a step that is necessary for controlling the stereochemistry of the cyclopropanation reaction? proceeded poorly (yields of the esters were 35-42%). Moderate yields (42-56%) were also obtained in the subsequent cyclopropanation with dimethylsulfoxonium methylide. A method for palladium(I1)-catalyzed cyclopropanation of olefins with diazomethane16did, however, work well. Thus, the N,N-dialkylated trans-2-phenylcyclopropyl(12) See, for example, ref 1 and: (a) Burger, A.; Fitchett, G. T. J . Am. Chem. SOC.1952, 74, 3415. (b) Kaiser, C.; Lester, B. M.; Zirkle, C. L.; Burger, A.; Davis, C. S.; Delia, T. J.; Zirngibl, L.

J . Med. Pharm. Chem. 1962,5, 1243. (c) Borne, R. F.; Forrester, M. L.; Waters, I. W. J. Med. Chem. 1977, 20, 771. (13) See, for example: (a) Kaiser, C.; Trost, B. M.; Beeson, J.; Weinstock, J. J. Org. Chem. 1965,30,3972 and ref 5c and 7a. (14) The use of various metal catalysts, in the cyclopropanation of styrene with ethyl diazoacetate, gave improved yields of ethyl 2-phenylcyclopropanecarboxylate,but had only minor effects on the stereoselectivity(transxis ratios 0.9-2.3); see: Anciaux, A. J.; Hubert, A. J.; Noels, A. F.; Petiniot, N.; TeyssiB, P. J . Org. Chem. 1980, 45, 695. Doyle, M. P.; Tamblyn, W. H.; Buhro, W. E.; Dorow, R. L. Tetrahedron Lett. 1981,22,1783. See also ref 15a. (15) (a) Paulissen, R.; Hubert, A. J.; TeyssiB, P. Tetrahedron Lett. 1972, 1465. (b) This reaction has also been used for stereospecific cyclopropanation of methyl trans-cinnamates; see: Evans, D. A.; Tanis, S. P.; Hart, D. J. J. Am. Chem. SOC.1981, 103, 5813 and ref 3b.

94 Journal of Medicinal Chemistry, 1988, Vol. 31, No. 1

amines were prepared as outlined in Scheme I. The required starting materials, the methyl trans-cinnamates 33a-d, were prepared by a Knoevenagel condensation of malonic acid and the appropriate substituted benzaldehydes followed by esterification of the acids.I6 The cyclopropanation (pathway I, Scheme I) proceeded smoothly and gave, after alkaline hydrolysis, good yields ( 7 6 9 2 % ) of the substituted trans-2-phenylcyclopropanecarboxylic acids. The subsequent Curtius rearrangement (pathway 11, Scheme I) was accomplished by use of diphenylphosphoryl azide22in tert-butyl alcohol. The tert-butyl carbamates thus formed were hydrolyzed in dilute hydrochloric acid to give the trans-2-phenylcyclopropylamines in moderate yields (48-72%). The trans stereochemistry of the amines was confirmed by analysis of the lH NMR spectra (Table I). Reductive m e t h y l a t i ~ nof~the ~ primary amines 2 and 12 with formaldehyde and NaCNBH3 afforded the N,Ndimethyl derivatives 3 and 13 (pathway 111, Scheme I). All other N,N-dialkylationsZ4were performed by treating the primary amines with the appropriate alkyl halide (pathway IV, Scheme I). The resulting N,N-dialkylated methoxy compounds were demethylated in 47% aqueous HBr to furnish the desired phenols. In order to prepare the enantiomers of compounds 8 and 18, we resolved the carboxylic acids 1 and 11 via the diastereomeric amides 34/35 and 36/37; the racemic carboxylic acids 1 and 11 were converted to the corresponding acyl chlorides, which were allowed $0 react with (R)phenylglycin~l.~~ The diastereomeric pairs of amides thus

Arvidsson et al.

354 / 3 2

2-OCH3

2/27

3-OCH3

formed, 34/36 and 36/37, were separated by use of flash chromatography.26 The choice of (R)-phenylglycinol as the alcohol component was based on its successful use in resolutions of other racemic carboxylic acids and the ease of cleavage of obtained diastereomeric amides under acidic conditions (N,O-acyl transfer Acid-catalyzed hydrolysis of 34-37 afforded, in high yields, the enantiomers of carboxylic acids 1 and 11, which were converted to the enantiomers of compounds 8 and 18 according to the reaction sequence outlined in Scheme I. The preparation methods and physical data of the synthesized compounds are summarized in Table 11. The absolute configurations of (-)-8 and (-)-18 were established by means of single-crystal X-ray analysis. Both compounds were found to have the 1R,2S configuration (Figure 1). An indication of the absolute configuration was obtained from the elution order of the diastereomeric amides 34/35 and 36/37 according to the guidelines postulated by Helmchen and c o - w o r k e r ~ . ~ ~

Pharmacology The compounds were tested in reserpinized rats by use of a previously described biochemical test method.ga BeCompounds 33a-d were prepared according to the procedure for the synthesis of methyl trans-3,4,5-trimetho~ycinnamate'~ havioral observations were made throughout the experiwith the following results (compound, yield from the correments. sponding benzaldehyde, bp/mp, reported bp/mp): 33a, 91%, The in vivo biochemical test method utilizes the well87-89 "C (0.05mmHg), 161-163 "C (13mmHg);1733b, 88%, established phenomenon of receptor-mediated feedback 120-126 "C (0.05-0.8mmHg), 305-307 "C (748mmHg);ls 33c, inhibition of the presynaptic neuronal a ~ t i v i t y . ~ ~ Thus, @*~ 79%,88-89 "C, 88 "C,lB89-90 0C;2033d, 70%,67-69 "C, 68-69 the synthesis rate of 5-HT is inhibited by 5-HT agonists. OC.21 Von Anwers, K. Justus Liebigs Ann. Chem. 1916,413, 253. Similarly, the synthesis of DA and norepinephrine (NE) Posner, T. J. Prakt. Chem. 1910,82,425. is inhibited by agonists activating DA and NE receptors, Grob, C. A.; Csapilla, J.; Cseh, G. Helu. Chim. Acta 1964,47, respectively. The 5-HTP accumulation, following de1590. carboxylase inhibition by means of (3-hydroxybenzyl)Heck, R. F. J. Am. Chem. Soc. 1968,90,5518. hydrazine (NSD 1015), was used as an index of the rate Adler, E.;Bjorkqvist, K. J. Acta Chem. Scand. 1961,5,241. of 5-HT synthesis in the three brain parts (limbic, stria(a) Shioiri, T.; Ninomiya, K.; Yamada, S. J. Am. Chem. Soc. tum, and hemispheres). The DOPA accumulation was 1972,94,6203.(b) Shioiri, T.; Yamada, S. Chem. Pharm. Bull. 1974,22,849. (c) Shioiri, T.; Yamada, S. Chem. Pharm. Bull. taken as an indicator of the rate of DA synthesis in DA1974,22,855.(d) Ninomiya, K.; Shioiri, T.; Yamada, S. Chem. predominated parts (i.e., limbic system, corpus striatum) Pharm. Bull. 1974,22, 1398. (e) Saikachi, H.; Kitagawa, T. and rate of NE synthesis in the NE-dominated remaining Chem. Pharm. Bull. 1977,25,1651. (f) This reagent did not hemispheral portions (mainly cortex). cause any epimerization in the Curtius reaction of cis-2-[3,4-

bis(benzyloxy)phenyl]cyclopropanecarboxylic acid; see ref 3b. Cannon, J. G.; Brubaker, A. N.; Long, J. P.; Flynn, J. R.; Verimer, T.; Harnirattisai, P.; Costall, B.; Naylor, R. J.; Nohria, V. J.Med. Chem. 1981,24,149. It should be noted that use of lithium aluminum hydride for reduction of an N-acyl derivative of trans-2-phenylcyclopropylamine led to opening of the cyclopropane ring; see: Kaiser, C.; Burger, A.; Zirngibl, L.; Davis, C. S.; Zirkle, C. L. J. Org. Chem. 1962,27,768. Ring opening has also been reported when the same reagent was used for reduction of Schiff bases of trans-2-phenylcyclopropylamine. However, alkali metal borohydrides were compatible in the reduction of Ncyclopropyl imines; see: Bumgardner, C. L.; Lawton, E. L.; Carver, J. G. J . Org. Chem. 1972, 37,407. Bolesov, I. G.; Surmina, L. S.; Yuzhakov, 0. N.; Levina, R. Ya. Zh. Org. Khim. 1974,10,1661;Chem. Abstr. 1974,81,135582j. (a) (R)-Phenylglycinol was prepared by reduction of D-(-)-aphenylglycine with LiAlH4 although it is commercially available (Sigma, Fluka, Aldrich, etc.). (b) The compound has been used for resolution of several carboxylic acids; see: Helmchen, G.; Nill, G.; Flockerzi, D.; Youssef, M. S. K. Angew. Chem., Int. Ed. Engl. 1979,18, 63.

Results and Discussion 5-HT-Receptor-Mediated Effects. The biochemical results for the compounds tested are given in Table 111. Compounds that decreased the brain 5-HTP levels also produced the 5-HT-like motor syndromem in the rat. The 5-HT-like behavior effects induced by 10 fimol/kg, sc, of 8 and 18 persisted after combined treatment with the monoamine depletor reserpine (5 mg/kg, ip) and the 5-HT synthesis inhibitor a-propyldopacetamide (H 22/54, 500 (26) Still, W.C.; Kahn, M.; Mitra, A. J. Org. Chem. 1978,43,2923. (27) Helmchen, G.;Nill, G.; Flockerzi, D.; Schuhle, W.; Youssef, M. S. K. Angew. Chem., Int. Ed. Engl. 1979,18,62. (28) Aghajanian, G.K.;Bunney, B. S.; Kuhar, M. J. In New Concepts in Neurotransmitter Regulation; Mandell, A. J., Ed.; Plenum: New York, 1973;p 119. (29) Jacobs, B. L. Life Sei. 1976,19, 777. Gerson, S. C.;Baldessarini, R. J. Life Sei. 1980,27,1435.


mg/kg, ip). This latter experiment rules out the possibility that indirect effects, such as, e.g., MA0 inhibition, would be responsible for the behavioral effects. Consequently, 8 and 18 appear to be direct-acting 5-HT-receptor agoni s t ~ .Similar ~ ~ results were obtained with the 5-HT-receptor agonist 8-hydroxy-2-(di-n-propylamino)tetralin (38, 8-OH-DPAT).31 A wealth of experimental data has con-

32

L Q -

R2

R3

RO

R,

8-OH

H

H

H

8-OCH3

H

H

H

42

7-OH

H

ti

H

42

6-OH

H

H

H

42

5-OH

H

H

H

42

5-OH

H

H

CH3

k4

5-OH

CHI

H

H

42

5-OH

H

CHI

H

firmed further the 5-HT-receptor activity of 38.31,32The similarities in pharmacological profiles of 8, 18,and 38 are also supported by studies showing that these agents facilitate male rat sexual behavior, tentatively by interacting with 5-HT receptors (compare ref 33 and 34). A hydroxy or methoxy group in the 2- or 3-position of the phenyl ring appears to be required for 5-HT-receptor-stimulating activity; the 4-hydroxy isomer 24 and the 3,4-dihydroxy isomer 28 did not decrease rat brain 5-HT synthesis (5-HTP formation) and failed to elicit clear-cut 5-HT-like behavioral effects even at high doses. The 2and 3-methoxy derivatives 7 and 17 were about 3-4 times less potent than their phenolic analogues 8 and 18, respectively. A similar potency ratio has been observed between the phenol 38 and its 0-methyl derivative 39.35

We have not ruled out the possibility that part of the biochemical effects of 8 and 18 may be interpreted as being due to a modest inhibition of MAO. However, this possibility seems less likely since N,N-di-n-propyl substitution abolishes such activities in a related series of oxygenated 2-aminotetralins. Compare: Hacksell, U.; Arvidsson, L.-E.; Johansson, A. M.; Nilsson, J. L. G.; Sanchez, D.; Lindberg, P.; Wikstrom, H.; Svensson, K.; Hjorth, S.; Carlsson, A,; Ask, A.-L.; Ogren, S.-0. Acta Pharm. Suec. 1986,23, 77 and ref 31. (a) Arvidsson, L.-E.; Hacksell, U.; Nilsson, J. L. G.; Hjorth, S.; Carlsson, A,; Lindberg, P.; Sanchez, D.; Wikstrom, H. J. Med. Chem. 1981,24,921. (b) Hjorth, S.; Carlsson, A.; Lindberg, P.; Sanchez, D.; Wikstrom, H.; Arvidsson, L.-E.; Hacksell, U.; Nilsson, J. L. G. J . Neural Transm. 1982,55, 169. See, for example: (a) Middlemiss, D. N.; Fozard, J. R. Eur. J. Pharmacol. 1983, 90, 151. (b) Gozlan, H.; El Mestikawy, S.; Pichat, L.; Glowinski, J.; Hamon, M. Nature (London) 1983, 305, 140. (c) Hamon, M.; Bourgoin, S.; Gozlan, H.; Hall, M. D.; Goetz, C.; Artaud, F.; Horn, A. S. Eur. J . Pharmacol. 1984, 100,263. (d) Tricklebank, M. D.; Forler, C.; Fozard, J. R. Eur. J . Pharmacol. 1984, 106, 271. (e) For a review on the pharmacology of 38, see: Arvidsson, L.-E. Drugs Future 1985,10, 916. Personal communication from Dr. Knut Larsson, Department of Psychology, University of Goteborg, Sweden. Ahlenius, S.; Larsson, K.; Svensson, L.; Hjorth, S.; Carlsson, A.; Lindberg, P.; Wikstrom, H.; Sanchez, D.; Arvidsson, L.-E.; Hacksell, U.; Nilsson, J. L. G. Pharmacol., Biochem. Behau. 1981, 15, 785.


The DA-receptor agonists367-hydroxy- (40),6-hydroxy(41),and 5-hydroxy-2-(di-n-propylamino)tetraline (42)do not seem to stimulate central 5-HT receptors even at relatively high dosesS3laHowever, the 8-hydroxy isomer 38 is a highly potent 5-HT-receptor agonist (see above).31a The hydroxy group, the benzene ring, and the nitrogen atom of 8 can be superimposed on the corresponding structural elements of the 5-HT-receptor-active 8hydroxy-2-aminotetralin derivative 38, thus resulting in a possible SAR rationalization of the similarities in the activity spectra displayed by these compounds. An equally good structural fit between the 5-HT-receptor agonists 18 and 38 is, however, not attainable. Instead, the 3hydroxyphenethylamine moiety of the 5-HT agonist 18 is superimposable on the corresponding structural element of the DA-receptor agonists 40 and 42,which do not seem to possess potent 5-HT-receptor-stimulating properties.31a The 5-HT-receptor-stimulating ability of 18 appears to be best rationalized by assuming that different conformations of 8 and 18 activate 5-HT receptor^.^' Variations of the N-alkyl groups of the 2-hydroxy and 3-hydroxy isomers of trans-2-phenylcyclopropylaminerevealed that the N,N-diethyl (6, 16) and the N,N-di-npropyl (8,18) derivatives were the most potent compounds in both series. The N,N-dimethyl derivatives 4 and 14 were about 15-18 times less potent than their N,N-di-npropyl homologues 8 and 18. The N,N-di-n-butyl derivatives 10 and 20 did not show any 5-HT stimulating properties. Similar structural requirements for the Nsubstituents have been observed previously in the 8hydroxy-2-aminotetralin series.35 The stereoselectivity of the potent 5-HT-receptoragonist 38 is very low; (2R)-38 is only twice as potent as its antipode in the 5-HTP accumulation t e ~ t . In ~ contrast, ~ ~ , ~ ~ virtually all 5-HT-receptor activity of 8 and 18 resides in the 1R,2S enantiomers. The difference in stereoselectivity between the 2-aminotetralin 38 and the trans-2-phenylcyclopropylamine derivatives 8 and 18 might be due to the steric bulk of the cyclopropyl methylene group of the 1S,2R enantiomers of 8 and 18, which may prevent proper alignments with 5-HT receptors (for a more detailed discussion, see ref 37). Effects on DOPA Accumulation. The 5-HT-receptor-stimulating 2-hydroxy isomers, the 3,4-dihydroxy analogues, and the 4-hydroxy-substituted compounds lacked significant dopaminergic or nonadrenergic properties even at the highest doses tested. Thus, the trans2-(2-hydroxyphenyl)cyclopropylaminederivatives are more selective than the 3-hydroxy isomers, which, in addition to 5-HT activity, weakly affected DA and NE systems; 14, 16, (hb18,and (-)-18 (0.8-1.5 pmol/kg, sc) reduced the DOPA levels in the DA-predominated brain parts (limbic system and corpus striatum) from 30% to 80% of control values and in the NE-dominated hemispheral portions from 53% to 78% of the control values. Larger doses of 16, (i)-l8,or (-)-18did not further reduce the DOPA levels. A high dose of 14 (50 pmol/kg, sc) did decrease (35) Arvidsson, L.-E.; Hacksell, U.; Johansson, A. M.; Nilsson, J. L. G.; Lindberg, P.; Sanchez, D.; Wikstrom, H.; Svensson, K.; Hjorth, S.; Carlsson, A. J. Med. Chem. 1984, 27, 45. (36) For recent reviews on DA-receptor agonists, see: (a) Nichols, D. E. In Dopamine Receptors; Kaiser, C., Kebabain, J. W., Eds.; ACS Symposium Series 224; American Chemical Society: Washington, DC, 1983; p 201. (b) Feenstra, M. G. P. Thesis, State University of Groningen, The Netherlands, 1984. (c) Cannon, J. G. Prog. Drug Res. 1985, 29, 303. (d) Kaiser, C.; Jain, T. Med. Res. Reu. 1985, 5, 145. (37) Arvidsson, L.-E.; Karlh, A.; Norinder, U.; Kenne, L.; Sundell, S.; Hacksell, U., J . Med. Chem., in press.


Aruidsson et al.

Table 11. Preparation and Physical Properties of N,N-Dialkylated Phenolic trans-2-Phenylcyclopropylaminesand Their Intermediates

~

no. 1 (+)-le (-)-le 2 (+)-2' (-)-2e 3 4 5 6 7 (+)-7e (-)-7e 8 (+)-a"

RO 2-OCHq

Y COOH

prepn meth I VI1 VI1 I1 I1 I1 I11 Vf IV V IV IV IV

yield,

~~~~

~

~~

recrystn solventsb

% mp, "C formulac 92 136-13ad 97 73.5-75.5 97 74.5-75.5d 193-194.5 A 63 76 215-217.5 A 215-217.5 A 78 70 168-170 A 191-192 62 B 56 155-156.5 B 134-134.5 48 B 67 158-159 B 90 174-175 C 174-175.5 88 C 79 B V 190-191.5 78 197.5-198.5 A V (-)-Se 75 A 197-198 V 9 B IV 95-97 80 10 B 156.5-157.5 V 78 11 I 94-959 89 (+)-lie oil VI1 94 (-)-lie oil VI1 97 12 155-156 I1 72 A (+)-12e 161-162 I1 68 D (-)-12e I1 D 161-162 71 13 169.5-171 I11 75 D 14 B 158-159 Vf 52 15 143-144 IV B 86 16 168.5-170 82 E V 17 125.5-127 B IV 73 (+)-17e 143.5-145.5 82 B IV (-)-17e IV 143.5-145.5 86 B 18 190.5-192 V F 79 (+)- 18" 203.5-204 77 V A (-)-lag A 203-204 64 V 19 62.5-64 B 73 IV 20 F 74 V 161.5-163 21 113-114.5h 84 I 22 I1 180-182' A 58 23 131-132.5 B 72 IV 24 185-187 C 65 V 25 75 I 107.5-108.5' D 48 178-180k I1 26 76 108-110 B IV 27 52 146.5-147 B V 28 Racemate unless otherwise denoted. *Recrystallization solvents: A, CH3CN-C2H50H; B, CH3CN-ether; C, CH3CN; D, CH3CNC2H50H-ether; E, 2-C3H70H-ether;F, CzHSOH-ether. CTheelemental analyses (C, H, and N) for all new compounds were within 3~0.4% of the theoretical values. dReported as partially resolved (ref 53; [aID-13.7"; mp 136-136.5 "C). eFor optical rotation, see Experimental Section. fDemethylation performed a t 100 "C (bath temperature) for 2.5 h. gReported without physical data (ref 54, 55). kLiteraturemp 112-113 "C (ref 56); literature mp 114-114.5 "C (ref 57). 'Literature mp 178.5-180.5 "C (ref 12b). ]Literature mp 105-105.5 "C (ref 12a). kLiterature mp of base 68-70 "C (ref 12a).

DOPA levels in the limbic system to about 55% of control values and the DOPA levels in the striatal brain parts to around 30% of controls. The effects after 14, 16, and 18 may or may not be due to direct DA-receptor stimulation. It should, however, be noted that classical full DA-receptor agonists36 like apomorphine decrease DOPA levels to around 20-30% of controls in the limbic and striatal brain parts and do not affect the DOPA levels in the hemispheral brain portions.38 Erhardt has suggested39that the inactivity of 30 at DA receptors is due to the steric bulk of the cyclopropyl group. (38) Hacksell, U.; Svensson, U.; Nilsson, J. L. G.; Hjorth, S.; Carlsson, A.; Wikstrom, H.; Lindberg, P.; Sanchez, D. J. Med. Chem. 1979,22, 1469. (39) Erhardt, P. W. J . Pharm. Sci. 1980,69,1059. Erhardt, P. W. Acta Pharm. Suec. Suppl. n2, 1983, 56.

The results obtained in the present investigation with compounds like 18 and 28 tend to support this idea, since 18, 28, and 30 probably can adopt "DA-receptor-active" phenethylamine conformation^.^^,^^ It is noteworthy that 43 appears to lack central DA-stimulating properties42and that the C1-methylated 2-aminotetralins 44 and 45 are (40) It has been suggested that near coplanarity of the phenyl ring and the ethylamine side chain of DA analogues is required for DA agonism; see ref 36c,d. (41) The barrier for internal rotation about the phenyl and the is small cyclopropyl ring of trans-2-phenylcyclopropylamines (see ref 37). (42) Hacksell, U.; Arvidsson, L.-E.; Johansson, A. M.; Nilsson, J. L. G.; Sanchez, D.; Andersson, B.; Lindberg, P.; Wikstrom, H.; Hjorth, S.; Svensson, K.; Carlsson, A. Acta Pharm. Suec. 1985, 22, 65.



Table 111. Effects of N,N-Dialkylated Phenolic trans-2-Phenylcyclopropylamineson Rat Brain 5-HTP Formation

5-HTP accumulation:b ED50,C8dfimol/ kg, sc hemispheres no." RO RN limbic striatum (cortex) 4 2-OH CH3 6.20 (2.15-17.9) 3.45 (0.78-15.3) 9.31 (0.78-15.3) 1.20 (0.32-4.54) 0.80 (0.31-2.08) C2H5 0.82 (0.40-1.68) 6 2-OH 7 2-OCH3 n-C3H7 0.93 (0.12-6.99) e 0.72 (0.06-9.29) 0.36 (0.10-1.25) 0.36 (0.11-1.17) 0.41 (0.10-1.61) n-C3H7 8 2-OH >50.0 n-C3H7 >50.0 14.5 (4.72-44.3) (+)-8 2-OH 0.28 (0.07-1.13) 0.26 (0.05-1.20) 0.38 (0.09-1.61) n-C3H7 (-1-8 2-OH >50.0 >50.0 n-C4H9 >50.0 10 2-OH 10.8 (3.40-34.4) 8.59 (2.38-31.0) 25.1 (4.45-140) 14 3-OH CH3 0.34 (0.12-0.96) 0.32 (0.10-0.94) 0.40 (0.15-1.08) 16 3-OH CZH6 17 3-OCH3 n-C3H7 2.06 (0.55-7.75) 1.17 (0.33-4.14) 1.43 (0.31-6.64) 18 3-OH n-C3H7 0.66 (0.31-1.41) 0.47 (0.19-1.13) 0.58 (0.10-1.65) (+)-18 3-OH n-C3H7 >50.0 >50.0 >50.0 (-)-lS 3-OH n-C3H7 0.34 (0.10-1.23) 0.28 (0.12-0.69) 0.35 (0.16-0.76) 20 3-OH n-C4H9 >50.0 >50.0 >50.0 24 4-OH n-C3H7 >50.0 >50.0 >50.0 28 3,4-(OH)z n-C3H7 >50.0 >50.0 >50.0 38 0.061f 0.06d 0.077f Racemate unless otherwise denoted. For experimental details, see ref 9. Dose giving a half-maximal decrease of 5-HTP formation in the rat brain part, estimated from a dose-response curve comprising four to six dose levels (n = 3-4). The maximal reduction of the 5-HTP level was empirically found to be 50% from the control levels (240 f 9 ng of 5-HTP/g of limbic tissue, 155 f 6 ng of 5-HTP/g of striatal tissue, and 136 f 6 ng of 5-HTP/g of hemispheral tissue, n = 26-30). dShown in parentheses are the 95% confidence limits of the ED,, values. e Available data do not allow proper evaluation of ED6, with confidence limits. f Values are from ref 35.

DA-receptor agonists of rather low potencies.43 In addition, 46, the C2-methylated analogue of 2-amino-6,7HO

o

a

C

H

3

"2

2 dihydroxytetralin (6,7-ADTN), seems to be inactive as a DA,-receptor agonist.4 Thus, introduction of steric bulk in the vicinity of the nitrogen frequently decreases or abolishes DA-receptor activity in phenethylamine-based DA-receptor agonists. Conclusions. In the present series of trans-2-phenylcyclopropylamines, aromatic C2- or C3-oxygen substituents and N,N-diethyl or N,N-di-n-propyl substituents gave potent 5-HT-receptor agonists. The 1R72Senantiomers of trans-2-(2-hydroxyphenyl)-N,N-di-n-propylcyclopropylamine (8) and the 3-hydroxy isomer 18 displayed 5-HT-receptor-stimulating activity, while the enantiomers were found to be inactive. In contrast, the potent 5-HTreceptor agonist 38 is only weakly stereoselective. The high stereoselectivity of 8 and 18 may be due to the steric bulk of the methylene group of the cyclopropyl ring, which might prevent a proper alignment with 5-HT receptors. The 2-hydroxy-substituted derivatives appear to be more selective pharmacologically than the other 5-HT-receptor agonists presented here; the latter compounds also seem (43) Hacksell, U.; Johansson, A. M.; Arvidsson, L.-E.; Nilsson, J. L. G.; Hjorth, S.; Carlsson, A,; Wikstrom, H.; Sanchez, D.; Lindberg, P. J . Med. Chem. 1984, 27, 1003. Johansson, A. M.; Arvidsson, L.-E.; Hacksell, U.; Nilsson, J. L. G.; Svensson, K.; Hjorth, S.; Clark, D.; Carlsson, A.; Sanchez, D.; Andersson, B.; Wikstrom, H. J. Med. Chem. 1985,28,1049. Johansson, A. M.; KarlBn, A.; Grol, C.; Sundell, S.; Kenne, L.; Hacksell, U. Mol. Pharmacol. 1986, 30, 258. (44) Nichols, D. E.; Jacob, J. N.; Hoffman, A. J.; Kohli, J. D.; Glock, D. J . Med. Chem. 1984,27, 1701.

to affect. central DA and NE systems. Experimental Section Chemistry. Melting points (uncorrected) were determined in open glass capillaries on a Thomas-Hoover apparatus. 'H NMR spectra recorded on JEOL F X go&, JEOL JNM-FX 200, or JEOL GX-400 spectrometers and mass spectra recorded at 70 eV on a LKB 9000 spectrometer were in accordance with the assigned structures. Optical rotations were obtained by use of a PerkinElmer 241 polarimeter. The elemental analyses (C, H, N) for the new substances (Mikro Kemi AB,S-750 19 Uppsala, Sweden) were all within f0.4% of the theoretical values. For purity tests, TLC was performed on fluorescent silica gel or alumina plates. For all compounds, only one spot (visualized by UV light and I2 vapor) was obtained. trans -2-(2-Methoxyphenyl)cyclopropanecarboxylicAcid (1). Method I. Diazomethane (CAUTION45)was prepared as a solution of 22.3 g (104 mmol) of Npreviously de~cribed;~' nitroso-N-methyl-4-toluenesulfonamide in ether (125 mL) was slowly added to a heated mixture of potassium hydroxide (6 g, 107 mmol), ether (10 mL), water (10 mL), and 2-(2-ethoxyethoxy)ethane (35 mL). The formed ether solution of diazomethane was continuously distilled into a stirred cooled (ice-salt bath) solution of 6.00 g (31.2 mmol) of methyl 2-methoxycinnamate (33a)16in ether (125 mL) and dichloromethane (50 mL),containing 50 mg of palladium(I1) acetate. The reaction mixture was kept a t -10 to -5 "C (bath temperature) until all diazomethane had been distilled (45 min). The cooling was discontinued after 1 h, and the reaction mixture was filtered (Celite) and concentrated in vacuo. The remaining oil was purified on a short alumina column eluted with ether. After evaporation of volatiles, the residual methyl trans-2-(2-methoxyphenyl)cyclopropanecarboxylate (6.4 g, =loo%) was hydrolyzed by use of 10 mL of a 50% sodium hydroxide solution in methanol (125 mL) (room temperature, 4 h). The reaction mixture was poured into a stirred mixture of 1L of water, 40 mL of concentrated HC1, and ice. The precipitated carboxylic acid was collected by filtration and washed with water until the filtrate had approximately neutral pH. The white solid was dried to afford 5.5 g (93%) of 1. (45) De Boer, T. J.; Backer, H. J. Organic Syntheses; Wiley: New York, 1963; Collect. Vol. IV, p 250.


trans-2-(2-Methoxyphenyl)cyclopropylamine (2). Method 11. A stirred solution of 5.00 g (26.0 mmol) of carboxylic acid 1, 6.19 mL (28.6 mmol) of diphenylphosphoryl azide, and 4.00 mL (28.7 mmol) of triethylamine in 50 mL of dry tert-butyl alcohol was heated at 90 "C (bath temperature) for 27 h. The volatiles were evaporated, and a 10% sodium carbonate solution was added. The alkaline solution was extracted with ether (4 X 80 mL), and the combined ether layers were dried (sodium sulfate), filtered, and concentrated in vacuo to afford the crude tert-butyl carbamate. It was purified by use of flash chromatographyz6on a silica gel column eluted with ether-petroleum ether (1:l)to give 5.07 g of the intermediate tert-butyl carbamate, which was treated with 125 mL of 1 N hydrochloric acid at 100 "C (bath temperature) for 5 h. The acidic solution was washed with ether (2 X 100 mL) and then alkalinized with saturated aqueous potassium carbonate. The amine was extracted with ethyl acetate (3 X 175 mL), and the combined organic layers were dried (sodium sulfate and then potassium carbonate), filtered, and concentrated in vacuo. The residue was dissolved in ether and treated with ethereal HC1. The precipitate was collected by filtration to afford 2.85 g (55%) of 2.HC1 as an off-white powder. Recrystallization from acetonitrile-ethanol-ether gave a n analytical sample. trans -2-(2-Methoxypheny1)-N,N-dimet hylcyclopropyla m i n e (3). Method 111. Compound 2.HC1 (0.40 g, 2.0 mmol) was dissolved in 12 mL of methanol, and 0.76 mL (10 mmol) of 37% aqueous formaldehyde and 0.42 g (6.0 mmol) of 90% sodium cyanoborohydride were added. The p H was adjusted to 6 by addition of acetic acid. The mixture was stirred a t room temperature for 24 h, and the volatiles were evaporated. Addition of 20 mL of a 10% aqueous sodium carbonate solution, extraction with ether (3 X 20 mL), drying (potassium carbonate), filtration, and evaporation of volatiles gave an oil. The crude product was purified on a silica gel column eluted with ether by use of flash chromatography. The product was treated with ethereal HCl to afford 0.29 g (63%) of 3.HC1 as a white solid. trans -2-(2-Methoxypheny1)-N,N-di-n -propylcyclopropylamine (7). Method IV. A mixture of 0.36 g (2.2 mmol) of 2,0.76 mL (7.9 mmol) of 1-iodopropane, and 1.50 g (10.9 mmol) of finely ground potassium carbonate in 10 mL of acetonitrile was stirred a t room temperature for 4 days. Ether (15 mL) was added, and insoluble material was removed by filtration. The filtrate was concentrated in vacuo, and the residue was chromatographed on an alumina column eluted with ether-petroleum ether (1:2). Precipitation of the base with ethereal HC1 afforded 0.41 g (66%) of 7.HC1 as a white solid. An analytical sample was obtained by recrystallization from acetonitrile-ether. trans -2-(2-Hydroxypheny1)-N,N-di-n -propylcyclopropylamine (8). Method V. A stirred solution of 0.45 g (1.6 mmol) of 7.HC1 in 15 mL of freshly distilled 47% aqueous HBr was heated for 2 h at 110 "C (bath temperature) under nitrogen. The solution was concentrated in vacuo, and 20 mL of ethanol-toluene (1:l)was added. Volatiles were evaporated, and the resulting solid was recrystallized from acetonitrile-ether to afford 0.40 g (80%) of 8.HBr as white crystals. ( l R f S ) - N - [ (R)-%Hydroxy- l-phenylethyl]-2-(2-methoxypheny1)cyclopropanecarboxamide (34) a n d ( l S , 2 R ) - N [(R)-2-Hydroxy-l-phenylethyl]-2-(2-methoxyphenyl)cyclopropanecarboxamide (35). Method VIa. Carboxylic acid 1 (8.00 g, 41.6 mmol) was converted to the corresponding acyl chloride by slow addition of thionyl chloride (8.00 mL, 110 mmol) at room temperature. The reaction mixture was stirred at 55 "C for 45 min and then concentrated in vacuo. Dichloromethane (25 mL) was added, and volatiles were evaporated. The residual acyl chloride of 1 in 75 mL of dichloromethane was slowly added (30 min) to a stirred ice-cooled solution of 6.30 g (45.9 mmol) of (R)-2-amino-2-pheny1ethanolz5 and 8.70 mL (62.4 mmol) of triethylamine in 250 mL of dichloromethane. After 30 rnin at 0 "C, the reaction mixture was left overnight a t room temperature and then washed with 250 mL of 1 N hydrochloric acid, 250 mL of saturated aqueous sodium carbonate, and 150 mL of saturated aqueous sodium chloride. The organic layer was dried (magnesium sulfate) and concentrated in vacuo. The residual brown-yellow solid was passed through a short silica gel column with ethyl acetate as eluant to afford 12.2 g of a mixture of 34 and 35 as a light yellow solid. The mixture was divided into three portions, and the diastereomers were separated on a silica gel (220 g) column

Arvidsson et al. Table IV. Crystal Data for (-)-8.HBr and (-)-8.HBr formula CIjH2,NO.HBr space group P212121 a, A 10.052 (1) b, A 7.653 (1) 21.247 (5) C,A P, deg dcdcd,g ~ r n - ~ 1.28 LL, cm-' 36.9

(-)-18.HBr (-)-18.HBr C,,H2,N0.HBr p2, 10.259 (1) 7.845 (5) 10.265 (10) 102.46 (1) 1.29 37.4

(88-mm o.d., 20-mL fractions) by use of flash chromatography. The samples were applied as solutions in chloroform, and the columns were eluted with ether-ethyl acetate (2:l). The three runs gave 4.91 g of the first-eluted compound (34) and 4.17 g of 35. Additional portions of 34 (0.83 g) and 35 (1.42 g) were recovered from the impure fractions (2.52 g). This procedure gave 5.74 g (44%) of 34 mp 147-148 "C; [alZzD -122.5' (c 1.33, CHCl,)] and 5.59 g (43%) of 35 [mp 123.5-124.5 "C; [alZzD +93.5" (c 1.31, CHCI,)]. (lR,%S)-N-[(R)-Z-Hydroxy-l-phenylethyll-2-(3-methoxypheny1)cyclopropanecarboxamide (36) a n d ( l S , 2 R ) - N [ (R)-2-Hydroxy-l-phenylethyl]-2-(3-methoxyphenyl)cyclopropanecarboxamide (37). Method VIb. Carboxylic acid 11 (8.00 g, 41.6 mmol) was converted to a mixture of the diastereomeric amides 36 and 37 by use of the same procedure as described for the preparation of 34/35. Prepurification on a short silica gel column gave 11.5 g of a yellowish solid. The mixture of 36 and 37 was divided into three portions and separated on a silica gel (220 g) column (88-mm o.d., 20-mL fractions) by use of flash chromatography. The samples were applied as solutions in warm ethyl acetate, and the columns were eluted with ethyl acetate. Fractions containing a mixture of 36 and 37 were pooled and chromatographed further. This procedure afforded 4.63 g (36%) of the first-eluted compound (36) [mp 147.5-149.0 "C; [alZzD -210.5" (c 1.14, CHCl,)] and 4.56 g (35%) of 37 [mp 155.0-156.5 "C; [alZzD+176.5" (c 1.24, CHCl,). Estimation of Diastereomeric P u r i t i e s of Amides 34-37. HPLC analyses of the compounds were performed by use of a Waters 5 Si 10 column (34 and 35) or a Waters 8 Si 5 column (36 and 37) with hexane-ethyl acetate--ethano1(80:15:5)as the mobile phase, working in the pressure range 1000-3000 psi and with a flow rate of 2 mL/min. Detections were made by a Waters Model 440 UV monitor. The diastereomeric excess (de), which was estimated by comparing peak areas (height X width at half-height), was found to be >98% de for 34 (tR = 2.2 min), 35 (tR = 3.0 min), 36 ( t R = 5.2 min), and 37 ( t R = 6.8 min). (1R ,2S)-2-(2-Methoxyphenyl)cyclopropanecarboxylic Acid [(-)-l], Method VII. A solution of 5.50 g (17.7 mmol) of 34 in 100 mL of dioxane and 100 mL of 3 N sulfuric acid was stirred a t 100 "C (bath temperature) for 24 h. The reaction mixture was concentrated in vacuo to half the volume and then extracted with ether (3 X 75 mL). The combined ether layers were dried (magnesium sulfate) and filtered. The volatiles were evaporated, and the remaining solid was chromatographed on a silica gel column eluted with ether to provide 3.30 g (97%) of (-)-l as a white solid. Absolute Configuration Determination of (-)-8.HBr a n d (-)-18.HBr by Single-Crystal X-ray Analysis. Crystals of (-)-8.HBr and (-)-lS.HBr were grown from acetonitrile-ethanol solutions. Crystals with the dimensions 0.30 X 0.10 X 0.02 mm [(-)-S.HBr] and 0.34 X 0.05 X 0.05 mm [(-)-lS.HBr] were used for data collection with an Enraf-Nonius CAD4F-11 diffractometer. The angular settings of 25 [(-)-8,HBr] and 12 [(-)-lS.HBr] reflections (6O < '6 < 38O) were measured to calculate the lattice parameters (cf. Table IV for crystal data). For each compound, two sets of independent reflections with 6' < 60 " were measured by the 8/26 scan method using monochromated Cu K Nradiation. Three intensity control reflections, which were measured every 2 h, indicated no crystal decay of (-)-8.HBr but a slight decay (3%) of the crystal of (-)-lS.HBr. For (-)-8+HBr,a total of 2569 reflections were recorded and, of these, 1001 reflections with I > 30(4 were considered observed. For (-)-lS.HBr, a total of 2528 reflections were recorded and, of these, 1860 with I > 3 0 ( 4 were considered observed. All intensities were corrected for Lorentz

Novel Central 5-Hydroxytryptamine Receptor Agonists and polarization effects but not for absorption or extinction. The structure was solved by a combination of the Patterson heavy atom method and direct methods using the program DIRD I F , ~which provided the non-hydrogen atom positions. All hydrogen atom positions except those for hydrogen atoms connected to methyl groups were obtained from Fourier difference synthesis maps. Refinement was carried out by the full-matrix least-squares method using anisotropic temperature factors for the non-hydrogen atoms. The hydrogen atoms were assigned a temperature factor equal to the corresponding parent atom Us,value. The hydrogen atom parameters were not refined. In order to determine the absolute configurations of (-)-8.HBr and (-)-18.HBr, we introduced anomalous dispersion factors47 for the non-hydrogen atoms. T h e atomic parameters of the non-hydrogen atoms for both enantiomers were then refined. C14 of (-)-18-HBr did not respond correctly to the refinement, and its parameters were therefore kept fixed during the final cycles of the refinement. Two sets of unique reflections (h,k,l, h-k,l) were used in the refinement, and nonobserved reflections were allowed to contribute when F, > F,. When the refinement of (-)-8-HBr was finished, the residuals for the 1R,2S and 1S,2R enantiomers were calculated to be R = 0.045 and R = 0.049 (R, = 0.058 and R, = 0.062), respectively. Corresponding residuals for the 1R,2S and 1S,2R enantiomers of (-)-18-HBr were R = 0.053 (R, = 0.064) and R = 0.058 (R, = 0.071),respectively. When Hamilton's test is used,& the ratio Rw(lS,2R)/Rw(1R,2S),which is 1.089 for (-)-8.HBr and 1.109 for (-)-18.HBr, is sufficiently great to reject the 1S,2R enantiomers at the 0.005 significance level. Furthermore, among the 41 Bijvoet pairs for the 1R,2S enantiomer of (-)-8.HBr and among the 52 Bijvoet pairs for the 1R,2Senantiomer of (-)-18.HBr for which F,(h,k,l) - F,(h-k,l) > 1.0, 38 and 48 of the respective F, differences had the same sign as the corresponding F, differences. The weighting scheme used in the later part of the refinement was w = 1/(1+ ((IF,! - 26)/28)2).4g The form factors used were those given by Cromer and Mann.so All calculations have been performed on a DEC-system-10 computer using mainly the X-ray 72 program system.51 The molecular conformation and atomic labeling scheme for the two compounds are shown in Figure 1. Optical Rotations. The resolved compounds presented in ~ ~ , Table I1 have the following optical rotations ( [ 0 1 ] ~ concentration, solvent): (+)-1, +194.7' (c 0.5, CHCl,); (-)-1, -196.5' (c 0.5, CHC13); (+)-2*HCl,+44.3' (C 1.7, CH3OH); (-)-2*HC1, -43.7' (C 1.6, CH3OH); (+)-7.HC1, +10.4' (C 1.1, CH3OH); (-)-7*HCl,-10.0' (c 1.3, CH30H); (+)-8.HBr, +7.5' (c 1.1, CH30H); (-)-8*HBr,-7.1' (C 1.1, CH3OH); (+)-11, +295.9' (C 1.2, CHC13); (-)-ll, -304.1' (C 1.1, CHC13); (+)-12*HC1,+72.4' (C 1.8, CHSOH); (-)-12*HCl, -69.8' (C 1.8, CH3OH); (+)-17*HCl, +75.2' ( C 1.3, CH3OH); (-)-17*HCl, -75.5' (c 1.3, CH30H); (+)-1&HBr, +67.0' (c 1.1, CH30H); (-)-l&HBr, -67.3' (c 1.1, CH,OH). Pharmacology. Animals used in the experiments were male rats of Sprague-Dawley strain (ALAB, Stockholm) weighing 200-300 g. T h e substances to be tested were dissolved in saline immediately before use, occasionally with the addition of a few drops of glacial acetic acid and/or moderate heating in order to Beurskens, P. T.; Bosman, W. P. J. H.; Doesburg, H. M.; Gould, R. 0.;Van den Hark, T. E. M.; Prick, P. A. J.; Noordik, J. H.; Beurskens, G.; Parthasarathi, V. Technical Report 1981/2; Crystallography Laboratory, Toernvooiveld,6525 ED Nijmegen, The Netherlands, 1981. Cromer, D. T.; Liberman, D. J. Chem. Phys. 1970,53, 1891. Hamilton, W. C. Acta Crystallogr. 1965, 18, 502. Mills, 0. S.; Rollet, J. S. Computing Methods and the Phase Problem in X-ray Crystal Analysis; Pergamon: New York, 1961; p 107. Cromer, D. T.; Mann, J. B. Acta Crystallogr., Sect. A: Cryst. Phys., Diffr., Theor. Gen. Crystallogr. 1968, 24, 321. Stewart, J. M.; Kruger, G. J.; Ammon, H. L.; Dickinson, C.; Hall, S. R. The X-ray System, version of June 1972; Technical Report TR-192, Computer Science Center, University of Maryland, College Park, 1972.

Journal of Medicinal Chemistry, 1988, Vol. 31, No. 1 99 obtain complete dissolution. Compound 28.HBr was dissolved under nitrogen in saline containing ascorbic acid. Reserpine was dissolved in a few drops of glacial acetic acid and made up to the volume with 5.5% glucose. Injection volumes were 5 or 10 mL/kg, and injection solutions had approximately neutral pH. The biochemical experiments were performed as previously described9" with one exception; the brain levels of 5-HTP and DOPA were analyzed by use of HPLC with electrochemical det e c t i ~ n .For ~ ~ biochemical results and experimental details, see Table 111.

Acknowledgment. We thank Torgny Fornstedt for skillful synthetic assistance and Ingrid Bergh, Boel Goransson, and Lucia Gaete for skillful assistance in the pharmacological testing. We are indebted to Sven-Olof Larsson (Pharmacia) for 200-MHz 'H NMR spectra and to Prof. Lennart Kenne for a 400-MHz lH NMR spectrum. The financial support from Bengt Lundkvists Minne, Astra Lakemedel AB, The Swedish Board of Technical Development, The Swedish Medical Research Council (Grants No. 155 and 7486), Knut and Alice Allenberg's Stiftelse, Anna Ahrenbergs Stiftelse, Magnus Bergvalls Stiftelse, Stiftelsen Lars Hiertas Minne, T. och R. Soderbergs Stiftelser, Gyllenstiernska Krapperups Stiftelsen, Helge Axson Johnsons Stiftelse, The Swedish Academy of Pharmaceutical Sciences, and the Medical Faculty, University of Goteborg, is gratefully acknowledged. Registry No. 1,110826-01-2; 1-(+), 110901-85-4;l-(-), 503403-7; 1 (methyl ester), 110826-41-0; 1 (tert-butyl carbamate), 110826-42-1; 1 (acyl chloride), 110826-43-2;2,110826-02-3; 2-(+), 110901-86-5; 2-(-), 110901-87-6; 3, 110826-03-4; 4, 110826-04-5; 4 (freebase), 110826-27-2;5,110826-05-6;6, 110826-06-7;6 (free base), 110826-28-3; 7, 110826-07-8; 7-(+), 110902-70-0; 7-(-), 110901-88-7; 7 (free base), 110826-29-4; 8, 110826-08-9; 8-(+), 111001-14-0;8-(-), 111001-15-1;8 (free base), 110826-30-7;8-(+) (free base), 110901-81-0; 8-(-) (free base), 110901-82-1; 9, 110826-09-0; 10, 110826-10-3; 10 (free base), 110826-33-0; 11, 96728-38-0; 11-(+), 110901-89-8; 1l-(-), 110901-90-1; 12, 110826-11-4; 12-(+), 110901-91-2; 12-(-), 110901-92-3; 13, 110826-12-5; 14, 110826-13-6; 14 (free base), 110826-34-1; 15, 110826-14-7; 16, 110826-15-8; 16 (free base), 110826-35-2; 17, 110826-16-9; 17-(+), 110901-93-4; 17-(-), 110901-94-5; 17 (free base), 110826-36-3; 18, 110826-17-0; 18-(+),111001-16-2; 18-(-), 111001-17-3; 18 (free base), 110826-37-4; 18-1(+) (free base), 110901-83-2;18-(-) (free base), 110901-84-3;19, 110826-18-1;20, 110826-19-2; 20 (free base), 110826-38-5; 21, 110826-20-5; 22, 110826-21-6; 23, 110850-48-1; 24, 110826-22-7; 24 (free base), 110826-39-6; 25, 110826-23-8; 26, 110826-24-9; 27, 110826-25-0; 28,110826-26-1;28 (free base), 110826-40-9;33a, 98288-15-4; )33b, 38693-90-2; 3 3 ~3901-07-3; , 3 3 4 30461-77-9;34,110826-31-8; 35, 110901-79-6; 36, 110826-32-9; 37, 110901-80-9; (R)-2-amino-2phenylethanol, 56613-80-0. Supplementary Material Available: Positional and thermal parameters, bond lengths, bond angles, and details about the determination of absolute configuration (8 pages); observed and calculated structure factors (38 pages). Ordering information is given on any current masthead page. Svensson, K. Thesis, Department of Pharmacology, University of Goteborg, Goteborg, Sweden, 1986. Nozaki, H.; Ito, H.; Tunemoto, D.; Kondo, K. Tetrahedron 1966, 22, 441. Kostikov, R. R.; Bobko, N. P.; D'yakonov, I. A. Reakts. Sposobn. Org. Soedin. 1971,8, 97; Chem. Abstr. 1972, 76, 30486. Daniewsk, A. R.; Kowalszyk-Przewloka, T. Tetrahedron Lett. 1982, 23, 2441. Trachtenberg, E. N.; Odian, G. J. Am. Chem. Soc. 1958, 80, 4015. Simmons, H. E.; Smith, R. D. J . Am. Chem. SOC.1959, 81, 4256.

novel central 5-hydroxytryptamine-receptor agonists - American

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