Open-Resonance-Assisted Hydrogen Bonds and Competing

Jul 19, 2018 - ... of this work was to investigate the role that π-delocalization plays in the ... of RAHB, and we address the role that acid–base ...
0 downloads 0 Views 750KB Size
Subscriber access provided by READING UNIV

Article

Open Resonance Assisted Hydrogen Bonds and Competing Quasiaromaticity Yen H. Nguyen, Bryan J. Lampkin, Amrit Venkatesh, Arkady Ellern, Aaron J Rossini, and Brett VanVeller J. Org. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.joc.8b01331 • Publication Date (Web): 19 Jul 2018 Downloaded from http://pubs.acs.org on July 19, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

Open Resonance Assisted Hydrogen Bonds and Competing Quasiaromaticity Yen H. Nguyen, Bryan J. Lampkin, Amrit Venkatesh, Arkady Ellern, Aaron J. Rossini, and Brett VanVeller* Department of Chemistry, Iowa State University, Ames, Iowa 50011, United States

ABSTRACT The delocalization of electron density upon tautomerization of a proton across a conjugated bridge can alter the strength of hydrogen bonds. This effect has been dubbed resonance-assisted hydrogen bonding (RAHB) and plays a major role in the energetics of the tautomeric equilibrium. The goal of this work was to investigate the role that π-delocalization plays in the stability of RAHBs by engaging other isomerization processes. Similarly, acid-base chemistry has received little experimental attention in studies of RAHB and we address the role that acid-base effects play in the tautomeric equilibrium. We find that π-delocalization and the disruption of adjacent aromatic rings is the dominant effect in determining the stability of a RAHB. INTRODUCTION The tautomeric equilibrium between enolimines (OH-form) and enaminones (NH-form, Figure 1A) plays a 1

critical role in biosynthesis and medicinal chemistry. For example, the Schiff bases of pyridoxal phosphate (Figure 1B) are important in the enzymatic transformations of amino acids, where proton 2-4

transfer between oxygen and nitrogen is often the first step of the catalytic cycle. enolimine drugs have been reported that act on the central nervous system 8

sirtinol (Fig 1B) is a deacetylase inhibitor that displays anticancer

9-14

5,6

Additionally, several +

15

and antiviral activity. The structure

of sirtinol features a Schiff base with 2-hydroxynaphthalene, which is important for drug action.

ACS Paragon Plus Environment

7

and K channels. Similarly,

8,16

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 2 of 21

Figure 1. A, tautomeric equilibrium of enolimine and enaminone. B, tautomeric examples.

The balance of the equilibrium between both tautomeric forms is partially dependent on the π17-22

delocalization in both of the rings indicated in Figure 1A (a and q).

The π-delocalization in ring q

favors the enaminone (NH-O) tautomer with a strong O•••H hydrogen bond termed a resonance-assisted hydrogen bond (RAHB). affected by substituents,

1,28

and this stabilization is

While the tautomeric equilibrium can be

in general, an increase in the aromaticity of ring q leads to a loss of

1,16,21,29,30

aromaticity in ring a,

1,21,23-27

23,24

and these results suggest that π-delocalization in ring a and q are coupled

to each other. This interplay was recently demonstrated to operate in the excited state as well.

31

To

investigate the coupling of π-delocalization further, we sought to “open up” the RAHB to engage a new tautomeric, quasiaromatic structure (Scheme 1). Previous studies have sought to entice the proton in Figure 1A away from the traditional RAHB but have not been successful.

Scheme 1. Tautomeric forms under consideration

ACS Paragon Plus Environment

16

Page 3 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

Intriguingly, the RAHB equilibrium of Figure 1A has not received much attention in the context of heterocycles. Most experimental studies have focused on all-carbon cycles except for some reports that employ the pyridine or quinolone nitrogen in the role of the Schiff-base in Figure 1A.

32-34

We hypothesized

that nitrogen heterocycles could suitably position Lewis basic atoms to coax the NH-O form to open up to an unprecedented NH-N form (Scheme 1). To test our hypothesis, we elected to focus on 1 and 2 (Scheme 1 and 2) for three reasons: (i) The geometry of the RAHB in ring q’ is different for both compounds. The 6-membered pyrazine ring in 1 presents a different hydrogen bonding geometry than the thiadiazole ring in 2. This geometric effect might alter the stability of the RAHB in q’. (ii) The basicity of the nitrogen atoms in each compound are different. The large difference in the pKaH values of the parent heterocycles, pyrazine and thiadiazole (Scheme 1), may also play a role in enticing the NH-O form to open up into the NH-N form. (iii) It has been proposed that π-delocalization within the RAHB heavily 35

perturbs the OH pKa, ultimately making analysis of the effect of acidity difficult

or impossible.

28

For this

reason, we sought to clarify our analysis and control for OH acidity by employing compounds with similar “initial” pKa values at the OH group. Towards this goal, we selected quinoxaline (3) and benzothiadiazole (4) because we measured similar OH pKa values for both compounds (Figure 2). The similar acidities of these base heterocycles also imply similar electron-withdrawing effects in both compounds.

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 4 of 21

Figure 2. Determination of pKa of compounds 3 and 4 by monitoring the onset of phenolate 36 formation. See SI for experimental details and data processing.

RESULTS AND DISCUSSION The synthesis of 1 and 2 commenced with diamine 5 as the starting point for both molecules. Closure of the pyrazine ring to form 6 was achieved via condensation with glyoxal, variant (7) was synthesized using thionyl chloride in sulfuric acid. 39

performed via Pd-catalyzed cross coupling to give 3 and 4.

38

37

while the benzothiadiazole

Substitution of –Cl for –OH was

Installation of the aldehyde required two 40

distinct conditions for each heterocycle. The Reimer–Tiemann reaction was required to convert 3 to 8, while the Duff reaction

41

42

using hexamethylenetetramine (HMTA) was employed to convert 4 to 9.

The

final Schiff bases were readily accessed upon condensation with aniline in ethanol to give 1 and 2.

Scheme 2. Synthesis of 1 and 2.

Examination of the NMR data of 1 and 2 provided insight into the dynamic tautomeric equilibrium proposed in Scheme 1 and reveals that the unprecedented NH-N form was present in both compounds. Various NMR solvents were used for the characterization of compounds 3–9, and assigned NMR spectra for 1 and 2 in CDCl3 used for analysis can be found in the SI (pages S3-S4). A. Evidence of only two forms in solution The OH and NH-O forms cannot be distinguished spectroscopically because proton exchange is fast on the NMR time-scale. Thus, a single peak is observed for the OH/NH-O ensemble (Figure 3, Table 1). For both 1 and 2, however, another form is observable in solution and corresponds to the NH-N form. Other tautomeric structures of 1 and 2 were disqualified because they were deemed to be too high in

ACS Paragon Plus Environment

Page 5 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

energy to warrant consideration based on their calculated energies (Scheme 3, vide infra and Figure S20).

1

1

Figure 3. NMR data for (A) 1 and (B) 2. One-dimensional H NMR (top) and H projections from the 2D 1

15

H{ N} HMBC experiments (bottom).

B. Dominance of NH-O within the OH/NH-O ensemble Both 1 OH/NH-O and 2 OH/NH-O exist predominantly (> 85%) in their NH-O forms, as evinced by 3

the following data summarized in Table 1: (i) A JHH splitting is observed between H7 and NH8 for both compounds (Figure 3, Table 1, row 1 and 2), indicating that the NH-O form dominates over the OH form. (ii) The chemical shift of C1 in both compounds (row 3) approaches a shift more similar to a carbonyl carbon (~190 ppm) than a phenolic carbon (~160 ppm). (iii) The chemical shift of

15

N8 (row 4) similarly

15

indicates the dominance of the NH-O form. The observed N chemical shifts are typical of those for conjugated NH (ca. –200 ppm), while an imine nitrogen chemical shift typically occurs between –25 to – 90 ppm.

43

Finally, Figure 4 displays the X-ray crystal structures of 1 and 2 with the position of H8

determined by dispersion-corrected plane-wave DFT calculations. Plane-wave DFT was used to take account of the periodicity of the crystal structure and more accurately locate the hydrogen atom within the

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 6 of 21

crystal structure. The experimentally measured C–C, C–N and C–O bond lengths correspond to those of the NH-O form and are consistent with the NH tautomer being the lower energy form in the crystal 1,44

state,

29,45-48

likely due to the higher proton affinity of N versus O.

Previously reported crystal structures

of Schiff-base RAHB compounds can show solid-state intermolecular interactions that directly contact the RAHB and cause the OH form to be lower in energy. In contrast to these previous reports,

49,50

however,

there are no clear intermolecular interactions with the RAHB of 1 and 2 evident in the crystal states (Figure S15 and S16). C. Evidence for the NH-N form 1

15

Inspection of the H{ N} HMBC NMR spectra reveals two distinct cross-peaks that reveal the 1

1

15

1

JNH( H8- N8) coupling (Figure 3). The major set of H NMR signals corresponds to the NH-O form and

the second, minor set of signals are assigned to the NH-N form in both 1 and 2. The ratio between the OH/NH-O ensemble and the NH-N form was measured directly from the proton signal integration and indicates that only 6% and 15% of 1 and 2 are found in the NH-N form, respectively (Table 1, row 5). It is noteworthy that the NH-N form opens up the classical RAHB in ring q to form an unprecedented, new 16

quasiaromatic ring q’.

The tautomeric state of the NH-N form is similarly support by the spectroscopic

evidence as for NH-O above (rows 1–4). Transfer of the proton to the nitrogen of the heterocycle (from N8-H to N3-H) was calculated to be too high in energy to be observable (Scheme 3). However, the 1

15

H{ N} HMBC NMR show a weak correlation between H8 and N3 at lower temperatures of 273 K,

consistent with the presence of a weak hydrogen bond (Figure S12).

ACS Paragon Plus Environment

Page 7 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

Table 1. Spectral data in support of tautomeric equilibrium and open RAHB.

Row 1. 1H7 ppm 2. 1H8 ppm 1 H7–1H8 coupling (Hz) 3. 13C1 ppm 4. 15N8 ppm 1 H8–15N8 coupling (Hz) 5. 1H7 Integr. Ratio (%) 6. Tautomeric NH-O% a 7. Overall population (%) 8. 9. 10. 11. 12. 13. 14. 15. 16.

NICS(1) a HOMED a HOMED q HOMED a (from xray) HOMED q (from xray) HOMED q’ HOMED c NICS(1) c NICS(1) b

9.64 15.24 9.0 179.3 –197.9 67.2 94 65

8.97 13.20 13.7 183.3 –233.8 89.5 6

9.05 14.19 10.7 184.0 –229.4 84.2 85 96

8.74 11.32 14.4 184.1 –243.3 91.2 15

33

61

6

4

81

15

–9.74 0.924 0.849

–4.27 0.762 0.892 0.873 0.953

–2.16 0.682

–6.66 0.876 0.860

–2.66 0.714 0.884 0.795 0.926

–1.26 0.646

0.977 –13.24 –10.02

0.983 –9.35 –9.04

0.977 –13.42 –12.91

0.983 –9.37 –11.20

0.937 0.983 –9.39 –8.50

All energetic (kcal/mol, 298 K), geometric and NMR shielding calculations performed at B3LYP/6311+g(d,p). NICS(1), HOMED values calculated for the 6-membered ring indicated by (a). NICS(1) values are reported as the negative isotropic shielding of a ghost atom 1.0 Å above the plane of the ring. HOMED: 0.0–0.4, 0.4–0.8, and 0.8-1.0 signify weak, moderate, and strong delocalization respectively. 15 See SI for methods to calculate HOMED. All N chemical shifts are referenced to CH3NO2. Experimental a 1 energy values measured by variable temperature NMR (see SI for details). NH-O% = [ JNH/(96 Hz) x 100%].

ACS Paragon Plus Environment

0.906 0.983 –9.54 –10.50

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 8 of 21

D. Quantification of tautomeric population 1

The H NMR spectra were integrated to quantify the relative amounts of OH/NH-O and NH-N and showed that the NH-N form represents 6% and 15% of the total molecules in solution for 1 and 2, 0

respectively (row 5). Based on these populations, we determined the ∆G difference between the OH/NHO ensemble and the NH-N form at 298K to be 1.62 and 1.01 kcal/mol for 1 and 2, respectively. The relative amount of OH versus NH-O form within the OH/NH-O ensemble (the NH-O%, Table 1

1, row 6), can be estimated by measuring how closely the JNH value of NH8 corresponds to an idealized 1

JNH value of 96Hz.

44,51,52

Numerous studies make use of

13

C and

17

O chemical shifts to estimate the

%NH-O form, but there is often disagreement between the methods used to calculate the final NH16,53-55

O%.

Indeed, recent theoretical studies suggest the NMR chemical shift of atoms within the RAHB is 56,57

not a reliable proxy for determining the equilibrium.

Furthermore, the electron withdrawing nature of

the heterocycles in 1 and 2 will also perturb the chemical shifts. Therefore, chemical shift is likely an inappropriate metric in our system. Thus, the %NH-O form within the OH/NH-O ensemble is most 1

accurately determined from the JNH coupling values that have historically been a more reliable indicator 3

than JH7–H8 coupling values.

58

Figure 4. Single crystal X-ray diffraction structures of 1 NH-O and 2 NH-O comparing experimental and calculated (in brackets) bond lengths. Heavy-atom bond lengths were calculated using molecular DFT B3LYP/6-311+g(d,p). The H8 position and N8-H8 bond lengths were predicted with dispersioncorrected plane-wave DFT calculations with heavy atom positions fixed from single X-ray diffraction. Bond lengths for 2 NH-O are an average of two structurally independent molecules in the asymmetric unit (s.u. are 0.004 for 1 NH-O and 0.003 Å for 2 NH-O). Anisotropic thermal ellipsoids depicted in (Figure S17).

ACS Paragon Plus Environment

Page 9 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

1

1

15

The JNH coupling values were measured for 1 and 2 using H{ N} HMBC NMR on a cryoprobe 1

800 MHz spectrometer and JNH was found to be 67.2 Hz and 84.2 Hz for 1 and 2 respectively (Figure 3, 1

1

Table 1 row 4). The measured JNH were converted to %NH-O via the equation %NH-O = [ JNH/(96 Hz) x 1

100%] (row 6). The JNH coupling values were also measured for the NH-N form in each case. Both 1 NH1

15

1

H and 2 NH-N show nearly identical JNH ( N8– H8) coupling values of 89.5 and 91.2 Hz, respectively (row 4), which suggests that both NH-N forms exist, to the same extent, with the proton predominantly on 1

N8. These measured JNH are consistent with computational results that show proton transfer to the nitrogen atom in the heterocycle is energetically unfavorable (Figure S20). These results indicate that the geometry of the hydrogen bond and the basicity of the heterocyclic nitrogen (see pKaH values of the parent heterocycles, Scheme 1) are unlikely to play a large role in the stability of the RAHB within NH-N because the heterocyclic nitrogen atoms do not appear to interact very strongly with H8. E. Energetic balance of tautomers The data from row 5 and 6 in Table 1 can be used to estimate the overall population of tautomers in solution (row 7) by multiplying the %NH-O for each OH/NH-O ensemble (row 6) by the integration value 1

for H7 (row 5). The values of NH-O% (Table 1, row 6) further confirm that the NH-O form is the majority form within the OH/NH-O ensemble for both 1 and 2. Interestingly, however, the 1 OH/NH-O ensemble is closer to parity, with the 1 NH-O form slightly dominant, while the 2 OH/NH-O ensemble is almost completely dominated by the 2 NH-O form. These results align with the smaller calculated energy difference between 1 OH and 1 NH-O (1.28 kcal/mol) versus 2 OH and 2 NH-O (2.96 kcal/mol) at 298 K. 0

Similarly, using variable temperature NMR, we determined the ∆G at 298K to be –1.62 and –1.01 kcal/mol for 1 and 2 respectively to confirm F. π-Delocalization and competing aromaticity We sought to investigate the reason behind the difference in the tautomeric equilibria of OH, NHO and NH-N between 1 and 2. As mentioned previously, the basicity of the heterocyclic nitrogen atoms (Scheme 1) does not appear to play a major role. Likewise, the inherent pKa of the OH groups in each heterocycle (3 and 4, Figure 2) are so similar, we propose that the acidity—and the electron-withdrawing capacity of the heterocycles—plays very little role in the balance of tautomers. We therefore turned to investigate the role that aromaticity in rings a, q and q’ might play in 1 and 2 (Table 1, rows 8–13).

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 10 of 21

Briefly, the aromaticity of a molecule can be estimated using computational chemistry to calculate the Nucleus Independent Chemical Shift (NICS) of a dummy atom placed 1 Å above the plane aromatic ring.

59

The more negative the NICS value, the more aromatic the ring (full NICS plots from 0–4 Å can be

found in the SI, Figure S21). Alternatively, the Harmonic Oscillator Model of Electron Delocalization (HOMED)

60

is a geometry-based measure of aromaticity in which the closer the HOMED value is to 1, the

more aromatic the molecule. An older index, HOMA

61

is prevalent in the literature of RAHB, and HOMA

values have also been calculated and can be found in the SI (Table S3). We elected to go with HOMED in place of HOMA because HOMED is derived from computationally determine structural parameter, and we sought to cancel out any potential computational errors with the computed structures for 1 and 2. In general, for compounds 1 and 2, ring a loses aromaticity upon tautomerizing from the OH form to the NH-O form as indicated by both NICS(1) and HOMED methods (Table 1, rows 8 and 9). The aromaticity in ring a for the OH forms, however, is greater for 1 than for 2. Thus, there is a smaller sacrifice of aromaticity in ring a for 2 upon tautomerization to the NH-O form, which explains why 2 exists in the NH-O form to a greater extent than 1. Likewise, a small increase in the aromaticity of quasiaromatic ring q is observed for both compounds upon tautomerization to NH-O (row 10). This observation aligns with previous reports that show ring q always displays significantly less change in its index of 16

aromaticity relative to the sacrifice of aromaticity in ring a to form the RAHB.

The overall trend of this

data is mirrored in the HOMED indices calculated from the X-ray crystal structures of 1 and 2 (rows 11 and 12). For the NH-N forms, the quasiaromatic ring q’ display high aromatic character according to HOMED indices (row 13), while ring a in each case loses still more aromatic character (row 9) relative to the NH-O forms. Again, we attribute the lower aromaticity in ring a of 2 as the reason that 2 displays more of the NH-N form in solution compared to 1.

ACS Paragon Plus Environment

Page 11 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

Scheme 3. Proton transfer to the heterocyclic ring nitrogen disrupts the aromaticity in both rings of the heterocycle. Energy differences calculated using DFT B3LYP/6-311+g(d,p).

These results suggest that acid-base effects play very little role in determining the equilibrium of 1 and 2, and the equilibrium is chiefly governed by aromaticity. We further propose that aromaticity considerations account for why proton transfer from N8 to N3 does not occur (described in section C above) (Scheme 3). The energy difference between 1 NH-N to 10 and 2 NH-N to 11 is too large to have an appreciable concentration at equilibrium. Tautomerization to form either 10 or 11 disrupts the aromaticity of both rings in the heterocycle because of π-delocalization to form the new q’ N8-HN3 quasiaromatic ring. We propose that this aromatic disruption is the main factor responsible for the large energy difference. Finally, rings b and c appear to be largely spectators in this isomerization process (Table 1, rows 14-16). HOMED values for ring c (row 14) indicate no change in aromaticity during tautomerization. The NICS(1) values (row 15), however, show a slight decrease in aromaticity of ring c upon tautomerization to the NH-O and NH-N forms which is likely due to changes in conjugation along the RAHB which alters conjugation effects and electron density within ring c. HOMED values were not calculated for ring b because N–S bonds have not been parameterized for analysis.

60

Alternatively, NICS(1) values were

calculated for compounds 1 and 2 (row 16). In general, ring b in both 1 and 2 experiences very little change in aromaticity across the different tautomers. Comparison between ring b of 1 and 2 is not appropriate because of the different ring sizes (6-membered vs 5-membered) in 1 and 2. 5-Membered rings typically appear 'more aromatic' than 6-member rings because the NICS measurement quantifies

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 12 of 21

the magnetic field at the center of the ring. Thus, the field is enhanced as the π-bonds move closer to the point where NICS is being measured.

CONCLUSION Collectively, these results indicate that resonance-assisted hydrogen bonds (RAHB) can be opened up provided there is a suitable Lewis base to stabilize the proton. This effect has not been observed before in the literature of RAHB, and we provide the first characterization of such an effect to open a RAHB to form quasi-aromatic ring q’. We also sought to investigate the effect that acid-base interactions might have on the RAHB and the equilibrium in Figure 1. We selected quinoxaline (3) and benzothiadiazole (4) as the base heterocycles for this analysis because our measurements revealed them to have very similar pKa values for their hydroxyl groups. These similar acidities, allowed us to isolate the aromaticity in ring a as the chief factor in determining the balance of tautomers in the OH/NHO equilibrium ensemble. Similarly, the difference in basicity of the heterocyclic nitrogen atoms in the NH1

N tautomer did not appear to influence the position of the proton in the RAHB (based on JN8–H8 coupling values in NH-N. These findings supports the primacy of aromaticity and π-delocalization in determining the tautomeric position in RAHB. EXPERIMENTAL SECTION Materials. Silica gel (40 µm) was purchased from Grace Davison. All solvents used for photophysical experiments were reagent grade. 4-chlorobenzene-1, 2-diamine (1) was purchased from Oakwood Chemicals, Palladium catalyst from Strem Chemicals, Hexamethylenetetramine from Alfa Aesar. All other reagents were purchased from Sigma Aldrich and used without further purification. 1

13

General Method. NMR Spectroscopy: H and C NMR spectra for all compounds were acquired in deuterated solvents (as indicated) on a Bruker Spectrometer at the field strengths reported in the text. 1

1

The chemical shift data are reported in units of δ (ppm) relative to residual solvent. 1D H NMR and H13

1

C HMBC NMR spectra were obtained using a 600 MHz Bruker Avance III spectrometer. Quantitative 1D

H NMR spectra were obtained at different temperatures for the thermodynamic analysis using long 1

15

recycle delays of 100 s (> 5 × T1) between subsequent scans. H- N HMBC NMR spectra were recorded using a 700 MHz Bruker Avance II NMR spectrometer. IR Spectroscopy: Solutions of 1 and 2 in CCl4

ACS Paragon Plus Environment

Page 13 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

were dropped onto KBr plates before collection of the spectrum on a Bruker Vertex 80 FTIR spectrometer. General Computational. All molecules considered were optimized at the nonlocal three-parameter 62,63

hybrid B3LYP level of theory

under the 6-311+g(d,p) basis set. Vibrational analysis of each structure

verified the existence of a minima due to the absence of imaginary frequencies. All calculations were carried out with the Gaussian 09 package.

64

Additionally, the hybrid CAM-B3LYP

65

and M06-2X

66

functionals were also for their ability to model charge transfer reactions and weak non-covalent interactions, respectively (Table S4). However, recent literature precedent has utilized the B3LYP functional to model proton transfer reactions and isomerizations.

67-69

HOMED values were obtained by using equation 1 where j indicates the bond type.



Ropt and α values used were from Raczyńska et. al. initial report of HOMED:

60

CC(Ropt = 1.394, α2i =

88.09), CN(1.334, 91.60), CO(1.281, 75.00). Plane-wave DFT calculations were performed on compounds 1 and 2 using CASTEP.

70

The X-ray crystal

structures were used to fix the heavy atom positions and the hydrogen atom positions were optimized. All 71

calculations used the Perdew-Burke-Ernzerhof (PBE) functional dispersion correction scheme

72

with the Tkatchenko-Scheffler (TS)

and ultra-soft pseudopotentials generated on-the-fly. The wavefunctions

were expanded using a plane-wave basis set with a kinetic energy cut-off of 630 eV. A Monkhorst-Pack -1

grid with a k-point spacing of 0.07 Å was used to calculate the integrals over the Brillouin zone. Synthesis of 6-chloroquinoxaline (6). The diamine 5 (428 mg, 3.00 mmol) was added to a solution of glyoxal in water and stirred at RT for 1h. Upon completion (determined by TLC), the reaction was 1

extracted with DCM, dried over MgSO4 and concentrated in vacuo. H NMR of the crude product matched previously reported literature.

37 1

H NMR (CDCl3, 600Hz): δ 8.83 (d, J = 6 Hz, 2H), δ 8.09 (s, 1H), δ 8.03

(d, J = 9 Hz, 1H), δ 7.70 (d, J = 8.4 Hz, 1H). Synthesis of quinoxalin-6-ol (3). Compound 6 (164 mg, 1.0 mmol), K2CO3 (414 mg, 3.0 mmol) and precatalyst Pd-t-BuXphos (40 mg, 5.0 mol %) (see SI for structure) were added together in a vial, then vacuumed and back-filled with nitrogen three times. Next, 5 mL of degassed DMF/water (5:1 v/v) was o

added. The reaction mixture was stirred at 115 C for 4 h. Upon completion, the reaction was poured into

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 14 of 21

1M HCl and extracted with diethyl ether three times. The combined organic fractions were dried over MgSO4 and concentrated in vacuo. The crude residue was triturated with hexane and DCM to give 3 1

(87.6 mg, 60% yield). H NMR of the product matched previously reported literature.

73 1

H NMR (DMSO,

600Hz): δ 10.56 (s, OH, 1H), δ 8.77 (d, J = 2.4Hz, 1H), δ 8.67 (d, J = 2.4 Hz, 1H), δ 7.90 (d, J = 9 Hz, 1H), δ 7.42 (dd, J = 9 Hz, 2.7 Hz, 1H), δ 7.24 (d, J = 2.7 Hz, 1H). Synthesis of (Z)-5-((phenylamino)methylene)quinoxalin-6(5H)-one (1). Compound 3 (1.0 mmol, 146 o

mg) and NaOH (4.0 mmol, 160 mg) were dissolved in 2.0 mL of water by heating to 100 C for 30 min. Then 1.5 mL chloroform was added with vigorous stirring. More NaOH was added to keep the pH of the reaction at 10 (determined with pH paper). Upon completion, the reaction was acidified to pH 6, and the solid was collected by filtration. The crude product was condensed with excess aniline (1.0 mL) in 5.0 mL o

DCM at 45 C, in a seal tube for 1h and monitored by TLC. The reaction was quenched with 1M HCl. The organic layer was separated, concentrated under vacuum and purified by silica gel column 1

chromatoghraphy (1:5 EtOAc:Hex) to obtain the final product 1 (99 mg, 40%). H NMR (DMSO, 600 Hz): δ 9.67 (s, 1H), δ 8.73 (s, 1H), δ 8.63 (s, 1H), δ 7.92 (d, J = 9.68 Hz, 1H), δ 7.58 (d, J = 7.4 Hz, 2H), δ 7.50 (t, J =7.4 Hz, 2H), δ 7.34 (t, J = 7.4 Hz, 1H), δ 7.15 (d, J = 9.68 Hz, 1H).

13

C NMR (DMSO, 150 Hz): δ

176.3, 153.5, 144.5, 141.6, 140.9, 137.1, 136.9, 129.9, 129.3, 127.0, 119.7, 108.0. HRMS (ESI, TOF) +

m/z: [M +H] Calcd for C15H11N3O 250.0975, found 250.0975. Synthesis of 5-chlorobenzo[c][1,2,5]thiadiazole (7). Based on a previously reported procedure,

38

4-

chlorobenzene-1,2-diamine (1.43 g, 10 mmol) was added to a flame dried round bottom flask and placed under nitrogen. SOCl2 (8.2 g, 5.0 mL, 68 mmol) and 0.220 mL of concentrated H2SO4 (405 mg, 4.1 mmol) were subsequently added. The reaction mixture was refluxed for 6 h and cooled to RT. The solution was diluted with water and extracted with EtOAc. The EtOAc phase was repeatedly washed with water until the aqueous phase was no longer acidic by pH paper. The organic phase was dried over MgSO4 and concentrated under vacuum. The crude mixture was purified by silica gel flash column chromatography 1

(5:1, Hexanes: EtOAc, Rf = 0.7) to afford the product 7 as a reddish brown solid (1.2 g, 70%). H NMR (CDCl3, 400 MHz) δ 7.85 (d, J = 4 Hz, 1H), δ 7.77 (d, J = 12 Hz, 1H), δ 7.38 (dd, J = 4 Hz, 6 Hz, 1H). Spectra of product match those previously reported.

38

ACS Paragon Plus Environment

Page 15 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

Synthesis of benzo[c][1,2,5]thiadiazol-5-ol (4). Compound 7 (17.0 mg, 0.10 mmol), KOH (17.0 mg, 0.30 mmol) and 3.96 mg (5% mmol) of Pd-t-BuXPhos precatalyst (see SI for structure) were added to a flame dried round bottom flask. The reaction flask was evacuated and back filled with nitrogen three times o

before 36 µL of degassed water:DMF (1:9 v:v) was added. The reaction was stirred for 18h at 80 C. Upon cooling to RT, the reaction was diluted with water, acidified with 1M HCl and extracted with EtOAc three times. The combined organic fractions were dried over MgSO4 and concentrated under vacuum. The residue was purified by silica gel flash column chromatography (start with 5:1 and gradient to 1:1, 1

Hexanes:EtOAc, Rf = 0.2) to afford a light yellow solid (10.6 mg, 70%). H NMR ((CD3)2CO, 600 Hz), δ 7.88 (d, J = 9.6 Hz, 1H), δ 7.37 (dd, J = 2.40 Hz, 9.6 Hz, 1H), δ 7.22 (d, J = 2.4 Hz, 1H). Spectra of product match those previously reported.

39

Synthesis of 5-hydroxybenzo[c][1,2,5]thiadiazole-4-carbaldehyde (9). Based on a previously reported procedure,

42

4 (76 mg, 0.50 mmol) and hexamethylenetetramine (HMTA) (70 mg, 0.50 mmol) were

dissolved in 1.0 mL of TFA in a round bottom flask at RT. After the consumption of starting material o

(tracked by TLC), the reaction was heated to 70 C for 48 h. Upon cooling to RT, the reaction was quenched with water and the mixture was extracted with ethyl acetate. The EtOAc portion was dried over MgSO4 and concentrated in vacuo. The residue was purified by silica gel column chromatography (1:1, Hexanes:EtOAc, Rf = 0.35) to afford a crude orange yellow product that was used without further 1

purification (44 mg, 49.0 %). H NMR (CDCl3, 600 MHz) δ 13.0 (br s, OH, 1H), δ 10.7 (s, CHO, 1H), δ 8.11 (d, J = 9.6 Hz, 1H), δ 7.33 (d, J = 9.6 Hz, 1H).

13

C NMR (CDCl3, 150 MHz) δ 193.7, 167.4, 154.2, -

150.3, 130.0, 124.8, 108.82. HRMS (ESI, TOF) m/z: [M - H] Calcd for C7H4N2O2S 178.9921, found [M-H] 178.9897. Synthesis of (Z)-4-((phenylamino) methylene)benzo[c][1,2,5]thiadiazol-5(4H)-one (2). Compound 9 (108 mg, 0.60 mmol) was dissolve in EtOH (3.0 mL, 0.50 M in 9). Aniline (54 mg, 0.60 mmol) was added, o

and the reaction was warmed to 40 C and tracked by TLC until completion. The reaction was poured into water and extracted with ethyl acetate. The EtOAc portion was dried over MgSO4 and purified by silica gel 1

flash chromatography (5:1, Hexanes:EtOAc, Rf = 0.45) to give a quantitative yield of 2 (153 mg, 99%). H NMR (CDCl3, 400 Hz): δ 14.2 (d, J = 10.4 Hz, 1H, H8), δ 8.90 (d, J = 11.2 Hz, 1H, H7), δ 7.63 (d, J = 9 13

Hz, 1H), δ 7.36 (m, 2H), δ 7.33 (m, 2H), δ 7.2 (t, J = 7.4 Hz, 1H). C NMR (CDCl3,100 Hz): δ 183.1,

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 16 of 21

+

156.4, 149.4, 149.0, 138.5, 131.9, 129.9, 129.3, 126.6, 118.3, 104.7. HRMS (ESI, TOF) m/z: [M +H] Calcd for C13H9N3OS 256.0539, found (M+H) 256.0568. ASSOCIATED CONTENT The Supporting Information is available free of charge on the ACS Publications website at DOI:

Structures of catalysts discussed in the main text, DFT calculations, computational analyses, pKa studies and NMR spectra. X-ray crystal structures have been deposited in the Cambridge Crystallographic Data Centre (1844550 and 1844551). AUTHOR INFORMATION Corresponding Author *Email: [email protected] ORCID Brett VanVeller: 0000-0002-3792-0308 Notes The authors declare no competing financial interest. ACKNOWLEDGMENTS We are grateful for support of this work by Iowa State University of Science and Technology and a Pfizer Global ASPIRE Award (WI216840). REFERENCES

! # $ %"

$ ! ()* 0 " ! 1 & " / 2 $')3'&, -, ! - / ! *4 ( 5, " 1 5 0 5 6 7 % , 8 8 9 " 5! / 1 5 $0 !' : ! / &" ! " 1 " $ ; $$ $ 0 ! 5 , 11 < "/ ! ! 1 !" / # $ %" 8' / = ! - 11 ! ((4) > .," ! & 6 ! $ " & / $ < 9 ? " +@@) ) -$ A & 6 " 6 B $! & 8 /$ & 6 " & 7$ / "!, $ 9 : ! 0 $ ! < 5 , 11 1 ': % ! " : / : / ': % ! " / 4@+ * " 6 $ : 5! " 8 7 ; " & 7 -"% "; 6 5 , "! A . 5 , 5 !, C' ." ! $$ ;" -, / &, " ! 19 0 $: !, &" /" ; 1 ' :$-, '6 !, $, ! < 8 ;,$ & ! ! / 5 $ ! 0 8 ! 8( -! " :; ! >@4@ +

$ ! ! & ' $ !" &, $$ ., !"

$

"

/

ACS Paragon Plus Environment

Page 17 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

4 5 !, D .,

/ $ F

=/

6 $ ; " "

? ! 5 =; ! ? = / 5 ! % $ : ! 0 ! 1 9 0 $ +' :$-, ':$ 2 $! "## $ % & EE> ; " . 6 ., $ $$ 8 6 / 1 5 $$ 6 $ $ = , % ! " 1 !, 5 "! B $ ! - 5 " ; # '( (FF(4 A ; 8 7 8 5 "! = ,%! " : ! " :;

5, " "/

, 6 "0 !0

5 ," % " 5 7 1 9 /' - /

!1 ! 1 .$ ! ! $ % &, E 8 A ! )" " ( E>) @ ! 8 ; ., ; 8 6 =G ! 6 : ,! 6 , ? 6 5 "! = , % ! " 5 "! $ = / 5 '7 " !, :"" ! !, :!! ! / '6 - 5 ; $ ; 8 . " . $$ * + 4* & ., . ? 8 B" & 6 !! 5 5 . % . B ,! " 6 A 8 . 7 5 "! = , % ! " = / . $$ !, / &)( : ! $ ! !," ;, "; ! ; !, 5 "! / 5 "!+ # , F>> + G ,, , B G! ? 8 / 9 : ? 9 ? ; , =! 6 : $ 1 " 5 "! . $$ " !, / ., " ! &" ! ! . "& ( / >) . $$ " ' >+( ( A 7 & " A ? 9 , A 8 7 7 A 8 A 8 9 A 8 , 11 ! 1 . % / " ! ! !, . -$ ! /8 ! ! $ = ,%! " 8 $ . $$ #* # +*+ > $$ : 6 6 $$ : / ; " A :$ , $ / " & 5 , ,/ / = ,%! 1 5 "! % 5 $$ 6 $ $ = / :- -! " !. " . $$ " ( ) / 5 9 ! 5 8 ; ? 9 " 6 5, " 8 ! 8 ? , 5 "! = , % ! " 5 "! $ = , % ! 8 - ! ! H" 8 0 -$ ! % = ,%! ;8 0= ! " $ % $ !" 5 ! : ! 0 ! " (( )*4 * : $ , $ " ; 6 -= 1$ 1 /B2 ! ': $ ! / 9'5 $ $ / $ / , " "!, '. I C " 0 ! 0 C I .,( 9,+ ,( ! " # $ %" 5 $ ! *+ ' ))EF 4 B$ " : $$ : $ 7 "" " 8 /" ; / 5! " $$ 6 / 1 / 5 , 11 .! % F() F B$ " : = !" $ $ " 8 /" ; / ; '8 /" 2 " $ 5 , 11 ! ! *+ *F* E B$ " : , $ : . $ $$ : 5! " / :" ! = - ! = !" $ $ " 8 /" ; / '8 /" 2 " $ ! / ! ! *+ EF* +@ B$ " : , $ : $ % 6 , B 5 5!" ! " $ / :" ! :- ! 1 ! " # $ %" 8 /" 2 :" $ 5 , 11 ! *+ E(E + " ; 6 5 ! $ 8 5! : A & $ 6 :" ! ! 1" !, H - ! 1 6 $ $ " !" < :--$ ! ! &$ " 5 ! *(F(

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

++ " % 8 5 0 J & ! 8 : ;K 6 :" ! ! $ & ' $ !" . - " !0! / 8' / ; &" - "! ! " 15 $ $/ $ < , L ! , " 1 :! 6 $ $ L! :--" , # # ' +E) +( $$ $$ B B "" !! H "! $ H 0/ 1 " ': ! / 8 /" ; ' / ; 1" ." ! $'5!" ! " . "" $ ! !, $B " 1 , ! ' ! B" ; ! @+( +> "! $ H $$ & B "" !! H $$ 0/ 1 " ': ! / 8 /" ; ' / ; + = ! " "" $ ! % ! ." ! $'5!" ! " / 5- !" - & " ! " F = !" $ $ "$ 8 /" ; ' / / (' " $' ('&" / $ >E 4 +) $$ & "! $ H B "" !! H $$ 0/ 1 " = !" $ $ " 9−8···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

ACS Paragon Plus Environment

Page 18 of 21

Page 19 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

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

ACS Paragon Plus Environment

The Journal of Organic Chemistry 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

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

ACS Paragon Plus Environment

Page 20 of 21

Page 21 of 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

The Journal of Organic Chemistry

" B

!

$

!< 9 ! $ / + !, " B *4 6 "! : / ; F *F 6 '= , % ! / 8 /" ;

B ! 6 G () 9

! $

$

" = B$ " "

: . / ;< : 9

/5 ! : &

!

! ; 1 B " 6@*'.$ + ) $ "! 1L ':" ! 8 /" ;

- ! ! $= 0 !; ! - 1= ! " ! H "

!, = !" $ $ " !, ,% 6 / $

*E 7 C 8 S, ; 8 ? A ; C 6 ? , " ; 1 !, 9 :// ! 0 ! ': ! / 8 /" ; /5 ! ! . 4@ .$ " 5 5 ; $$ 6 & "/ . A 8 - & A &" % "! 6 A 1 & 6 . 6 7 ## + )*4 4 & "/ A & " " ", 1 6 " $ / " / ! :--" 2 6 / 5 -$ ! 2 '' (F*) 4+ ! , : 5 , 11$ " 6 : " ! 6 $ $ " H " $ = ! " 1" " /'5! ! $ !" ! / B" ':! 1 " ! ! 2 4( C 5 8 7 ; 8 7 S 6 . -- "'. ! $ / 8 /" 1 8 ! " :" $ 8 $ / / " 6 $/ . / ! (>E(

ACS Paragon Plus Environment

' F)()

! ! 2 $ !