Delivered at the Crystal Engineering to Crystal Growth: Design and Function Symposium, ACS 223rd National Meeting, Orlando, Florida, April 7-11, 2002
CRYSTAL GROWTH & DESIGN 2002 VOL. 2, NO. 5 381-385
Phase-Shifting Interferometry for the Study of the Step Dynamics during Crystallization of Proteins Olga Gliko,† Nicholas A. Booth,† Eva Rosenbach,‡ and Peter G. Vekilov*,† Department of Chemical Engineering, University of Houston, Houston, Texas 77204, and Center for Materials Research, University of Alabama in Huntsville, Huntsville, Alabama 35899 Received April 15, 2002
ABSTRACT: We have developed a novel phase-shifting interferometry technique for high-resolution in-situ investigations of the unsteady dynamics of growth steps during the crystallization of proteins. The phase-shifting algorithm employs five-image sequences captured with a phase shift of π/2; digital processing of the sequence allows reconstruction of the surface morphology with a depth resolution
