Production Temperature Effects on the Structure of Hydrochar-Derived

May 27, 2018 - Shanghai Institute of Pollution Control and Ecological Security, ... derived from hydrothermal liquefaction, and it carries good potent...
1 downloads 0 Views 1MB Size
Subscriber access provided by La Trobe University Library

Sustainability Engineering and Green Chemistry

Production Temperature Effects on the Structure of Hydrocharderived Dissolved Organic Matter and Associated Toxicity Shilai Hao, Xiangdong Zhu, Yuchen Liu, Feng Qian, Zhi Fang, Quan Shi, Shicheng Zhang, Jianmin Chen, and Zhiyong Jason Ren Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b04983 • Publication Date (Web): 27 May 2018 Downloaded from http://pubs.acs.org on May 27, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 32

Environmental Science & Technology

1

Production Temperature Effects on the Structure of Hydrochar-derived

2

Dissolved Organic Matter and Associated Toxicity

3

Shilai Hao,1 Xiangdong Zhu,1, 2,* Yuchen Liu,1 Feng Qian,1 Zhi Fang,3 Quan Shi,3 Shicheng

4

Zhang,1,4* Jianmin Chen,1 Zhiyong Jason Ren2

5

1 Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of

6

Environmental Science and Engineering, Fudan University, Shanghai 200433, China

7

2 Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder,

8

Boulder, CO 80309, United States

9

3 State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, China

10

4 Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China

11 12

* Corresponding author, Tel/fax: +86-21-65642297; E-mail: [email protected]

13

(Xiangdong Zhu), [email protected] (Shicheng Zhang).

14 15

Word Count:

16

Words: 4664

17

Figures: 6 × 300 = 1800 words

18

Total: 6464

1 ACS Paragon Plus Environment

Environmental Science & Technology

19

ABSTRACT

20

Hydrochar is a carbonaceous material derived from hydrothermal liquefaction, and it carries

21

good potential as a new material for environmental applications. However, little is known about

22

the dissolved organic matter (DOM) associated with hydrochar and the consequences of its

23

release. The relationship between the production temperature and the characteristics of DOM

24

released from hydrochar, as well as the associated biotoxicity were investigated using a suite of

25

advanced molecular and spectroscopic tools. With the increase in production temperature, the

26

resulted hydrochar-based DOM contained higher content of phenols and organic acids but less

27

sugars and furans. Meanwhile, the molecular structure of DOM shifted to lower molecular

28

weight with higher organic contents containing < 6 O atoms per compound, aromatics, and

29

N-containing substances. While low-temperature hydrochar-derived DOM showed minimal

30

biotoxicity, increase in production temperature to 330 °C led to a great rise in toxicity. This

31

might be attributed to the increased contents of phenols, organic acids, and organics containing
240 °C).35, 36 In addition,

66

the degree of decarboxylation, dehydration, and condensation reactions that produce large

67

molecules increases greatly with increasing HTL temperature. As a result, high HTL

68

temperatures yield highly aromatic and low molecular weight (MW) compounds.37 Due to the

69

strong hydrophobicity of these compounds, significant amounts of these compounds accumulate

70

on the hydrochar surface rather than in HTL aqueous solutions.20 Thus, the molecular

71

composition of hydrochar-based DOM could be strongly affected by temperature. This change in

72

molecular composition would ultimately result in significant differences in the biotoxicity of

73

hydrochar-based DOM. If the relationship between HTL temperature and hydrochar-based DOM

74

properties and the associated toxicities are clarified, upstream control of the hydrochar

75

production process to decrease its potential environmental risk would be possible. Therefore,

76

studying the effects of temperature on the molecular structure and potential biotoxicity of 4 ACS Paragon Plus Environment

Page 4 of 32

Page 5 of 32

77

Environmental Science & Technology

hydrochar-based DOM is important.

78

Due to the compositional complexity of hydrochar-based DOM, single conventional analytical

79

methods such as gas chromatography-mass spectrometry (GC-MS) cannot fully characterize the

80

molecular and structural properties of DOM. Recently, electrospray ionization (ESI) Fourier

81

transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has proven to be

82

advantageous in analyzing DOM from different aquatic environments.38-42 Due to its ultrahigh

83

resolution, a much broader scale of compounds in DOM and accurate molecular formulas can be

84

determined unambiguously, including those containing hetero-atoms (e.g., N and S). This

85

method has allowed researchers to determine the molecular fingerprints of complex DOM, such

86

as aromaticity and double-bond equivalent (DBE), and track the molecular changes of DOM. By

87

combining ESI FT-ICR MS with other conventional characterization methods, such as

88

excitation-emission matrix fluorescence spectroscopy (EEM), the molecular structures of

89

hydrochar-based DOM can be clearly identified, and the potential mechanism of toxicity of

90

DOM can be better interpreted.

91

Therefore, this study comprehensively characterized the structural differences and biotoxicity

92

of hydrochar-based DOM under different conditions with the aim to provide deeper

93

understanding of this material to guide future production and application. A series of hydrochar

94

products were prepared under different HTL temperatures (180 – 330 °C), and the characteristics

95

of DOM released into an aquatic environment were investigated using various advanced

96

molecular and spectroscopic tools. The biotoxicity of DOM was investigated based on

97

cyanobacteria (Synechococcus sp), and possible growth inhibition mechanisms were discussed. 5 ACS Paragon Plus Environment

Environmental Science & Technology

98

Materials and Methods

99

Hydrochar and DOM Samples

100

Hydrochar was produced from 100 mesh bamboo (from Zhejiang Province) through

101

hydrothermal liquefaction. In a typical experimental run, 15 g (dry weight) of bamboo and 150

102

mL of water were loaded in the autoclave, heated up to the desired temperature for 60 min, and

103

then cooled with tap water to room temperature. The resulting solid product was recovered by

104

filtration and was then freeze-dried to obtain the hydrochar material. The bamboo-derived

105

biochar was produced through pyrolysis under N2 flow of 500 mL min-1 at 500 °C for 60 min.

106

The characterization methods of hydrochar were described in Supporting Information (SI) Text

107

S1. The release of DOM from hydrochar was performed by soaking 0.1 g of hydrochar sample in

108

50 mL of ultrahigh-quality Milli-Q water in a 60-mL amber glass vial. This mixture was shaken

109

at 150 rpm for various durations (0.5, 1, 2, 4, 8, 12, and 24 h) to examine the release kinetics of

110

DOM. To observe the effects of solution pH on the release of DOM, the solution pH was

111

adjusted with a small volume of 0.1 M HCl or NaOH solution (See SI Table S1). The contact

112

time was controlled at 24 h to evaluate the effects of hydrochar production temperature variation

113

on its DOM release. The DOM solution was collected by filtering through a 0.45-µm

114

polytetra-fluoroethylene membrane.

115

Characterization of DOM Samples

116

DOM samples were analyzed using a pH meter, a total organic C (TOC) analyzer, and

117

ultraviolet-visible (UV-vis) spectrometry. Ion chromatography (IC) was used to analyze sugars 6 ACS Paragon Plus Environment

Page 6 of 32

Page 7 of 32

Environmental Science & Technology

13

118

and organic acids.

C nuclear magnetic resonance (NMR) spectra were used to analyze the

119

molecular structure of the DOM, while GC-MS was used for phenol and furan compound

120

analysis (See Text S2 for detailed methods).

121

ESI FT-ICR MS analysis was performed on a Bruker Apex-ultra FT-ICR mass spectrometer

122

equipped with a 9.4 T superconducting magnet and Apollo II ESI source. The DOM samples

123

were diluted in methanol with a concentration of about 0.1 mg/mL and directly injected into the

124

electrospray source at 180 µL/h using a syringe pump. The operating conditions for negative-ion

125

formation consisted of 4.0 kV spray shield voltage, 4.5 kV capillary column introduced voltage,

126

and -320 V capillary column end voltage. The 4M word size was selected for the time domain

127

signal acquisition. A total of 128 scan FT-ICR data sets were accumulated to enhance the

128

signal-to-noise ratio and dynamic range. The methodologies used for FT-ICR MS mass

129

calibration, data acquisition, and processing are described elsewhere.43 Baseline scans of

130

methanol were performed to ensure that the instrument was clean before analyzing the samples.

131

EEM fluorescence spectroscopy spectra were recorded using a fluorometer (Aqualog;

132

Horiba-Jobin Yvon, USA). The fluorometer was set up as follows: the excitation wavelength was

133

incrementally increased from 240 to 550 nm in 3-nm intervals, with emission monitoring from

134

220 to 600 nm at 2-nm intervals for each excitation wavelength. Quinine sulfate standards were

135

used to calibrate the EEM spectra, and the fluorescence intensities were expressed in quinine

136

sulfate equivalent units. After removing additional regions dominated by Rayleigh and Raman

137

peaks, as well as regions without fluorescence, parallel factor (PARAFAC) modeling was

138

conducted with non-negative constraints using MATLAB ver. 8.5.0197613 (R2015a) with PLS 7 ACS Paragon Plus Environment

Environmental Science & Technology

139

toolbox ver. 8.0.1.44

140

The cyanobacteria (Synechococcus sp. (PCC 7942)) used in the assay were obtained from the

141

Institute of Hydrobiology, Chinese Academy of Sciences (Wuhan, China). Bioassays of DOM

142

using cyanobacteria were performed in 50-mL triangular flasks containing 15 mL of BG-11

143

medium and 10 mL of different DOM samples to reach a final DOM concentration of ~ 16 mg

144

C/L. The flasks were illuminated in an incubator for 7 days with a light: dark cycle of 12:12 h.

145

Cyanobacterial growth was monitored daily by measuring the change in absorbance at 680 nm

146

(algal chlorophyll absorbance peak) using a BioTek Synergy HT multimode microplate reader

147

(BioTek Instruments; Winooski, VT). Microphotographs of selected cyanobacteria were taken

148

under a microscope (Eclipse Ti-s; Nikon, Japan) with a CCD camera (DS-Ri1; Nikon, Japan).

149

Results and Discussion

150

Carbon Transformation in Hydrochar and Hydrochar-based DOM Release

151

Figure 1 presents the basic elemental analyses of the hydrochar samples based on FTIR, NMR,

152

and thermogravimetry. As the hydrochar production temperature increased, the graph describing

153

hydrochar properties changed visibly. These changes included a decline of H/C and O/C atomic

154

ratios based on a Van Krevelen (VK) diagram, as well as lower aliphatic and higher aromatic

155

components as indicated by the FTIR (Text S3) and 13C NMR results. This was attributed to the

156

increase in the degree of cellulose and lignin cracking at higher HTL temperatures. As seen in the

157

thermograms (Figure 1c), high-temperature-derived hydrochar showed higher recalcitrance as

158

suggested by a lower weight loss and higher retention weight.45 This higher recalcitrance 8 ACS Paragon Plus Environment

Page 8 of 32

Page 9 of 32

Environmental Science & Technology

159

indicated that hydrochar had higher aromaticity.45 Overall, hydrochar properties were strongly

160

affected by the production temperature, which is expected to further affect hydrochar-based

161

DOM release into the environment.

162

The time-dependent DOM (mg C/g of char) release kinetics indicated that the amount of DOM

163

released from hydrochar sharply increased within the initial 2 h and then plateaued after 12 h

164

(Figure 2a). The calculated partitioning coefficients for DOM-like material that partitioned from

165

hydrochar to water at varying pH indicated that only 3.5 – 6.5 % TOC in hydrochar was

166

extractable (Table S2). Such variation of partitioning coefficients resulted from the synthetic

167

effects of various surficial properties of hydrochars and aquatic environments. Generally, higher

168

temperatures promoted DOM release from hydrochar, possibly caused by a gradual elevation in

169

the amount of biomass cracking during the HTL process. With increasing hydrochar production

170

temperature, more cracked hydrophobic organic compounds were enriched on the surface of

171

hydrochar, resulting in the release of more DOM. Notably, when compared with biochar

172

produced by pyrolysis at 500 °C, hydrochar released at least ten times more DOM than biochar

173

(Figure 2a). This suggested that when used as an environmental amendment, hydrochar should

174

be more carefully characterized and evaluated compared with biochar from the perspective of

175

global C cycle and environmental impacts. In addition, alkaline conditions resulted in greater

176

DOM release (Figure 2b), especially at pH 10, suggesting that alkalinity facilitated the release of

177

DOM from hydrochar. This could be attributed to a larger amount of acidic organic compounds

178

released from hydrochar in alkaline solutions due to the acidity of hydrochar.20

9 ACS Paragon Plus Environment

Environmental Science & Technology

179

Molecular Composition of DOM Released from Hydrochar Produced at Different

180

Temperatures

181

The chemical composition (i.e., compounds detectable with GC-MS and IC) of DOM changed

182

significantly under different production temperatures (Figure 2c). With increasing temperature,

183

the total amount of phenols and organic acids increased, the amount of sugar compounds

184

decreased, while furans first increased followed by a decrease. Table S3 showed the results of

185

quantitative analysis of each compound as a function of hydrochar production temperature. At

186

lower temperatures between 180 – 240 °C, hemicellulose cracking dominated the process with

187

more furans and sugars generation, while lignin was not readily hydrolyzed. Elevating the

188

temperature above 240 °C enabled further degradation of furans and production of organic

189

acids.46, 47 In addition, lignin was gradually cracked to produce more phenols with increasing

190

hydrochar production temperature. These intermediates partially deposited on the surface of

191

hydrochar and contributed to hydrochar-based DOM. This was confirmed by the strong linear

192

correlation between hydrochar production temperature and the total amount of phenols in DOM

193

(Figure 2d). Through GC-MS and IC analyses, the low molecular weight volatile organic

194

compounds (i.e., furan and phenol) and the common organic acids (i.e., acetic acid) were well

195

detected and quantified. However, these two techniques cannot work well for high molecular

196

weight organic compounds with high boiling points. Furthermore, the C distribution of DOM

197

indicated that up to 95.8% DOM was not identified and quantified with these conventional

198

chromatography and mass spectral analyses (Figure S1). This was due to the fact that many polar

199

organic compounds with high molecular weight cannot be analyzed by GC-MS and IC 10 ACS Paragon Plus Environment

Page 10 of 32

Page 11 of 32

200

Environmental Science & Technology

techniques.

201

To address this limitation, state-of-the-art negative ion ESI FT-ICR MS was used to further

202

characterize the molecular composition of hydrochar-based DOM. The MW distribution of DOM

203

ranged from 150 to 550 Da (Figure S2). Thousands of molecular formulas of DOM were

204

identified by assigning molecular formulas (CaHbNcOd) to each peak (ESI FT-ICR MS molecular

205

information file).48 It should be noted that ionization with negative ESI (-ESI) is suitable for

206

polar compounds with acidic functionalities49 and less-volatile components that cannot be

207

detected by GC-MS. Generally, high-temperature hydrochar-based DOM contained more types

208

of organic compounds (Table S4). For example, 773 organic compounds were identified in

209

hydrochar-based DOM (180 °C), while 1301 organic compounds were detected in

210

hydrochar-based DOM (270 °C), indicating the progression of depolymerization reactions.

211

However, when the hydrochar production temperature exceeded 270 °C, the number of organic

212

compounds detected slightly decreased. This could be due to the re-polymerization of small

213

organic compounds under the high temperature and high pressure HTL reactions. ESI FT-ICR

214

MS greatly expands the scope of molecular information for DOM compared with conventional

215

analyses. Furthermore, the peak of the m/z distribution representing the major compounds in

216

DOM shifted from MWs of 350 – 400 to MWs of 250 – 300 with increasing HTL temperature

217

(Figure S3a), which was consistent with the change of average MW of DOM (Table S4). The

218

proportion of molecules with MWs of 350 – 400 showed a strong negative linear correlation with

219

hydrochar production temperature (Figure S3b), supporting the initial observation that higher

220

hydrochar production temperature promoted depolymerization reactions during HTL and resulted 11 ACS Paragon Plus Environment

Environmental Science & Technology

221

in a shift towards molecules with lower MWs. Figure S4 showed a scale-expanded view of the

222

ESI FT-ICR MS mass spectra of DOM at m/z masses of 417 and 418. It was observed that DOM

223

was composed of CHO and CHON compounds and higher production temperature led to higher

224

molecular diversity. These results further confirmed the effects of hydrochar production

225

temperature on the molecular composition changes in hydrochar-based DOM.

226

Figure 3 and Figure S5 depicted the VK diagrams (from the ESI FT-ICR MS data) for the

227

CHO and CHON molecules, respectively. The number of CHON compounds steadily increased

228

in DOM as a function of hydrochar production temperature, which became evident at 330 °C

229

(Figure S5). Table S4 also indicated that 330 °C hydrochar based-DOM had the highest

230

percentage of CHON compounds. This indicated that more CHON compounds accumulated on

231

the hydrochar surface under high temperature as a result of increased destruction of larger

232

N-containing molecules.50 Similarly, DOM released from high-temperature hydrochar contained

233

higher diversity of CHO compounds (Figure 3). It should be noted that the proportion of CHO

234

compounds with low O/C and H/C ratios (i.e., aromaticity index) increased in DOM with

235

increasing HTL temperature, indicating the progression of deoxygenation and dehydrogenation

236

during HTL. Meanwhile, according to the aromaticity index AImod,51 most compounds (68~76%)

237

in DOM were aliphatic compounds (Figure 4a). Moreover, increasing hydrochar production

238

temperature resulted in less release of aliphatic compounds but more aromatic compounds. This

239

was understandable, as hemicellulose and cellulose were degraded into aliphatic compounds at

240

low HTL temperatures, while the cracking of lignin resulted in more aromatic compounds at high

241

HTL temperature. The changes in aromatic compounds were in agreement with the changes in 12 ACS Paragon Plus Environment

Page 12 of 32

Page 13 of 32

Environmental Science & Technology

242

O/C values in the VK diagrams. Overall, DOM released from hydrochar produced at high

243

temperatures contained more diverse molecules and aromatic compounds.

244

The detailed variations of each class of species among the types of DOM were further

245

analyzed. As HTL temperature increased, Ox class molecules (organic compound with x oxygen

246

atoms) from hydrochar-based DOM shifted to organics that contained < 6 O atoms per

247

compound (Figure 4b and Table S4). The dominant Ox classes were O6 to O9 at lower

248

temperatures (180 – 240 °C) and O4 to O5 at high temperatures (270 – 330 °C). A similar shift of

249

< 6 O atoms per compound in the N1Ox class (organic compound with 1 nitrogen atom and x

250

oxygen atoms) was also observed (Figure 4c). These shifts in Ox and N1Ox classes indicated that

251

hydrochar produced at higher HTL temperatures accumulated more compounds with < 6 O

252

atoms as a result of increased depolymerization and deoxygenation. Notably, there was an

253

increase in the proportions of N1O4 and N1O5 class compounds in DOM from hydrochar

254

produced at 330 °C compared with other hydrochar-based DOM. Thus, hydrochar-based DOM

255

(330 °C) exhibited distinct toxic effects compared to other DOM.

256

To further investigate the DOM structure, relative abundance maps of DBEs as a function of C

257

number were plotted for each chemical class for different DOM (Figure S6). Generally, as

258

hydrochar production temperature increased, the diversity of each class compound increased and

259

showed a shift to higher DBE areas. This indicated that these species became more scattered and

260

unsaturated, further illustrating the enhancement of diversity and aromaticity in DOM molecules.

261

With exact C number and DBE value, the changes in organic acid compounds in different

262

hydrochar-based DOM could be further elucidated. More long-chain organic acids (e.g., 13 ACS Paragon Plus Environment

Environmental Science & Technology

263

C14H28O2, tetradecanoic acid, C15H30O2, pentadecanoic acid, and C17H34O2, heptadecanoic acid)

264

were released from the surface of high-temperature hydrochar (Figure 4d). In general, with

265

increased HTL temperature, the MWs of the molecules in DOM, the amount of sugar compounds,

266

the ≥ 6 O class compounds, and aliphatic compounds decreased, while the proportion of CHON

267

compounds, < 6 O class compounds, acidic compounds, and aromatic and unsaturated

268

compounds increased.

269

Structural Properties of DOM from Hydrochar Produced at Different Temperatures

270

The UV-vis spectrum of DOM had a characteristic absorbance at 232 nm corresponding to

271

π-π* transitions of aromatic C=C bonds (Figure S7). DOM derived from hydrochar produced at

272

higher HTL temperatures showed higher absorbance values, indicating that it contained

273

substances with more aromatic structures, which was in agreement with the FT-ICR MS

274

molecular composition analysis.

275

The EEM spectra showed a marked increase in fluorescence intensity in DOM from hydrochar

276

produced at high temperatures (Figure S8). Moreover, the range of fluorescence increased with

277

increasing hydrochar production temperature, indicating that more types of optical substances

278

were released from the hydrochar. The EEM-PARAFAC analysis of the fluorescence spectra

279

supported a model containing four components (I, II, III, and IV) (Figure 5). Components I and

280

II presented excitation/emission maxima at 250/325 and 240/325 nm, respectively. These two

281

components could be substances with phenolic, less aromatic, or protein-like structures which

282

are located in the low excitation/emission region of the EEM.27 Component III featured two 14 ACS Paragon Plus Environment

Page 14 of 32

Page 15 of 32

Environmental Science & Technology

283

peaks at 240/450 nm and 340/450 nm, which are classified as UVC and UVA humic-like

284

substances (hydrophobic fraction with large molecular size).52 Component IV showed a peak in

285

270/300 nm, which had lower emission wavelengths than components I and II. This red shift in

286

the excitation/emission maximum in component IV may be associated with an increased

287

aromaticity and higher molecular weight relative to components I and II.27, 53, 54 The standard

288

EEM spectra of the compounds in DOM from the GC-MS and IC analyses were compared to the

289

four PARAFAC components to clarify the connection between the optical properties and

290

molecular composition of DOM (Figure S9 and Table S5). Most phenols showed fluorescence

291

absorptions similar to the components I, II, and IV, while furans, sugars, and organic acids

292

exhibited weak fluorescence absorption. This suggested that the main optical substances in DOM

293

were phenol-like aromatic compounds. The relative abundances of the model components (based

294

on released concentrations) showed that component I accounted for > 40% of the optical

295

substances in DOM (Figure S10a). Notably, the relative abundance of component IV showed a

296

marked increase in DOM from hydrochar produced at 330 °C compared to other DOM (Figure

297

S10a). Furthermore, component IV may have been derived from < 6 O atoms compounds in the

298

N1Ox class, which was strongly supported by the positive correlations between the percentage of

299

N1O5 and N1O4 classes and relative abundance of component IV (Figure S10b, c). Component IV

300

may also have been derived from increased aromatic Ox and N1Ox class compounds, since ESI

301

FT-ICR MS results showed that each class compound showed a shift to higher DBE areas (more

302

aromatic) with increasing production temperature. Overall, aromatic substances, especially

303

phenol-like compounds, were the most abundant optical substances in DOM. With increasing 15 ACS Paragon Plus Environment

Environmental Science & Technology

304

hydrochar production temperature, the changes in the molecular compositions of DOM (e.g.,

305

2,6-dimethoxyphenol) were in accordance with the changes in the relative abundance of their

306

attributed modeled components.

307

The solution-state

13

C NMR spectra of DOM were used to further investigate the molecular

308

structure of DOM (Figure S11). The compositions of functional moieties were compiled in Table

309

S6. Aromatic C (90 – 146 ppm) and alkyl C (0 – 45 ppm) were the main C-containing functional

310

groups in all DOM samples (Table S6). Typically, with increasing hydrochar production

311

temperature, hydrochar-based DOM contained more aromatic C and alkyl C but less O-alkyl

312

derivatives. This is because the feedstock (bamboo) underwent more intensive dehydration and

313

condensation at higher temperature that resulted in the corresponding change in DOM structure.

314

In addition, the proportion of ester functional groups (COOR, 165 – 185 ppm) increased slightly,

315

in accordance with the increase of carboxylic acids (e.g., tetradecanoic acid) in DOM as a

316

function of hydrochar production temperature. Overall, the NMR-derived characteristics of the

317

DOM samples were in agreement with the results of the UV-vis and EEM analyses.

318

Bioassay Shows Biotoxicity of DOM from Hydrochar Produced at Different Temperatures

319

The bioassay results showed that most hydrochar-based DOM (210 ~ 300 °C) caused slight

320

inhibition of cyanobacterial growth (Figure 6a). Interestingly, hydrochar-based DOM (180 °C)

321

and biochar-based DOM (500 °C) showed no biotoxic effects on cyanobacteria, rather it

322

exhibited a stimulatory effect. However, when production temperature was increased from

323

210 °C to 300 °C, the biotoxicity of hydrochar-based DOM increased with an inhibition rate 16 ACS Paragon Plus Environment

Page 16 of 32

Page 17 of 32

Environmental Science & Technology

324

from 6-15%. At the highest production temperature (330 °C), hydrochar-based DOM completely

325

inhibited cyanobacterial growth. The microphotographs of the growth of cyanobacteria

326

confirmed the trend of DOM biotoxicity (Figure 6b). From the perspective of biotoxicity, more

327

attention should be placed on the environmental risk of high temperature-derived hydrochar (>

328

330 °C). Furthermore, hydrochar production needs to be optimized to ensure the environmental

329

safety of hydrochar.

330

The difference in biotoxicity among different DOM samples could be related to several distinct

331

changes of the chemical components. In a previous study, the toxicity of DOM from biochar was

332

believed to be caused by phenolic compounds or low-MW organic acids.24, 55 Based on the

333

molecular composition changes in the hydrochar-based DOM under different temperatures, the

334

increased amounts of phenol-like compounds and organic acids in DOM partially contributed to

335

the increased toxic effects on cyanobacteria. In addition, hydrochar-based DOM (330 °C) had the

336

highest abundance of N1Ox compounds with < 6 O atoms and component IV. This suggested that

337

< 6 O class compounds and CHON compounds in DOM may contributed to the increased

338

toxicity of DOM from high-temperature hydrochar. Furthermore, the proportions of N1O5, N1O4,

339

and N1O3 classes positively correlated with the inhibition rate of cyanobacteria (Figure S12 and

340

Figure 6c), suggesting that N1Ox class compounds with < 6 O atoms strongly inhibited

341

cyanobacterial growth. This might be explained by the N1Ox class compounds’ ability to easily

342

penetrate into the cell membranes of cyanobacteria, inducing strong biotoxicity. However, each

343

compound’s exact contribution to toxicity remained to be further analyzed due to the complex

344

mixture of different compounds in DOM; the toxic effects could be due to a combination of 17 ACS Paragon Plus Environment

Environmental Science & Technology

345

multiple mechanisms. Overall, these findings indicated that phenols, organic acids, and

346

N-containing compounds with < 6 O atoms contribute to the inhibition of cyanobacterial growth.

347

Environmental Implications

348

This study showed for the first time that hydrochar generated from HTL can release high

349

concentrations of DOM in aquatic environments. This will significantly impact hydrochar

350

production and its environmental applications. The hydrochar produced at high production

351

temperatures released more phenols, organic acids, aromatic substances, as well as species with

352

< 6 O atoms. This was likely due to the progression of cracking reactions during the HTL process,

353

such as dehydration and deoxygenation. Markedly, hydrochar-derived DOM (330 °C) exhibited

354

the greatest inhibition of cyanobacterial growth, which was probably related to the increase in

355

N1Ox class compounds or higher aromaticity compounds. The comprehensive molecular and

356

spectroscopic characterizations used in this study offered a critical dataset of hydrochar-based

357

DOM for future research at a molecular level. Such understanding of the DOM composition,

358

structure, and toxicity provided insights on the potentials and challenges of the eco-friendly use

359

of hydrochar materials. Therefore, material optimization and functionalization should be

360

conducted in a more controlled manner with high functionality and low toxicity in the future of

361

hydrochar commercialization. While this study revealed the characteristics of DOM from

362

hydrochar, future studies are needed to investigate HTL operation methods in order to generate

363

hydrochar with less toxicity without affecting bio-oil quality. It is also necessary to develop

364

post-treatment methods to detoxify hydrochar and improve beneficial use of this material. 18 ACS Paragon Plus Environment

Page 18 of 32

Page 19 of 32

365

Environmental Science & Technology

ACKNOWLEDGEMENTS

366

This research was supported by the National Key Research and Development Program of

367

China (Grant No. 2017YFC0212205), the National Key Technology Support Program (Grant No.

368

2015BAD15B06), the National Natural Science Foundation of China (Grant No. 21577025), the

369

International Postdoctoral Exchange Fellowship Program of China Supported by Fudan

370

University and the State Key Laboratory of Heavy Oil Processing.

371

Supporting Information

372

The hydrochar characterization procedures are provided in Text S1. The analytical procedures

373

to determine the basic characteristics of DOM are presented in Text S2. FTIR analysis of

374

hydrochar is shown in Text S3. The volume of 0.1 M HCl or NaOH solution added to adjust

375

DOM solution pH is shown in Table S1. The partitioning coefficients for DOM-like material that

376

partitioned from hydrochar to water at varying pH are in Table S2. The main chemical

377

compounds detected by GC-MS and IC are in Tables S3. The characteristic information of DOM

378

by ESI FT-ICR MS is listed in Table S4. The fluorescence peak positions of the main identified

379

compounds in DOM are shown in Table S5. Relative proportions of the chemical functional

380

groups are listed in Table S6.

381

The C distribution, broadband ESI FT-ICR MS spectra, MW distribution, VK plots for the

382

CHON molecular formulas, and expanded ESI FT-ICR mass spectra of DOM are listed in

383

Figures S1 – S5. The DBE versus C number distribution of the different DOM are shown in

384

Figure S6. The UV-vis spectra, EEM, and

385

S11. The correlations between cyanobacterial inhibition rate and proportions of N1Ox class

13

C NMR spectra of DOM are listed in Figures S7 –

19 ACS Paragon Plus Environment

Environmental Science & Technology

386 387 388

compounds are shown in Figure S12. The molecular formulas and intensity for the compounds in DOM by ESI FT-ICR MS was attached as an Excel file (FT ICR MS Information).

389

20 ACS Paragon Plus Environment

Page 20 of 32

Page 21 of 32

Environmental Science & Technology

390

REFERENCES

391

(1) Qian, F.; Zhu, X.; Liu, Y.; Shi, Q.; Wu, L.; Zhang, S.; Chen, J.; Ren, Z. J. Influences of temperature and metal on

392

subcritical hydrothermal liquefaction of hyperaccumulator: implications for the recycling of hazardous

393

hyperaccumulators. Environ. Sci. Technol. 2018, 52, 2225-2234.

394

(2) Zhu, X.; Liu, Y.; Qian, F.; Lei, Z.; Zhang, Z.; Zhang, S.; Chen, J.; Ren, Z. J. Demethanation trend of hydrochar

395

induced by organic solvent washing and its influence on hydrochar activation. Environ. Sci. Technol. 2017, 51,

396

10756-10764.

397

(3) Lehmann, J. A handful of carbon. Nature 2007, 447, 143-144.

398

(4) Glaser, B.; Lehmann, J.; Zech, W. Ameliorating physical and chemical properties of highly weathered soils in the

399

tropics with charcoal – a review. Biol. Fert. Soils 2002, 35, 219-230.

400

(5) Xiao, X.; Chen, B.; Zhu, L. Transformation, morphology, and dissolution of silicon and carbon in rice

401

straw-derived biochars under different pyrolytic temperatures. Environ. Sci. Technol. 2014, 48, 3411-3419.

402

(6) Han, L.; Ro, K. S.; Sun, K.; Sun, H.; Wang, Z.; Libra, J. A.; Xing, B. New evidence for high sorption capacity of

403

hydrochar for hydrophobic organic pollutants. Environ. Sci. Technol. 2016, 50, 13274-13282.

404

(7) Uchimiya, M.; Wartelle, L. H.; Klasson, K. T.; Fortier, C. A.; Lima, I. M. Influence of pyrolysis temperature on

405

biochar property and function as a heavy metal sorbent in soil. J. Agr. Food Chem. 2011, 59, 2501-2510.

406

(8) Libra, J. A.; Ro, K. S.; Kammann, C.; Funke, A.; Berge, N. D.; Neubauer, Y.; Titirici, M. M.; Fühner, C.; Bens,

407

O.; Kern, J.; Emmerich, K. H. Hydrothermal carbonization of biomass residuals: a comparative review of the

408

chemistry, processes and applications of wet and dry pyrolysis. Biofuels 2011, 2, 71-106.

409

(9) Manyà, J. J. Pyrolysis for biochar purposes: a review to establish current knowledge gaps and research needs.

410

Environ. Sci. Technol. 2012, 46, 7939-7954.

411

(10) Sohi, S. P.; Krull, E.; Lopez-Capel, E.; Bol, R. Chapter 2 - a review of biochar and its use and function in soil.

412

Adv. Agron. 2010, 105, 47-82.

413

(11) Funke, A.; Ziegler, F. Hydrothermal carbonization of biomass: a summary and discussion of chemical

414

mechanisms for process engineering. Biofuel. Bioprod. Bior. 2010, 4, 160-177.

415

(12) Brewer, C. E.; Schmidt-Rohr, K.; Satrio, J. A.; Brown, R. C. Characterization of biochar from fast pyrolysis and

416

gasification systems. Environ. Prog. Sustain. Energ. 2009, 28, 386-396.

417

(13) Huggins, T. M.; Pietron, J. J.; Wang, H.; Ren, Z. J.; Biffinger, J. C. Graphitic biochar as a cathode

21 ACS Paragon Plus Environment

Environmental Science & Technology

418

electrocatalyst support for microbial fuel cells. Bioresour. Technol. 2015, 195, 147-153.

419

(14) Kambo, H. S.; Dutta, A. A comparative review of biochar and hydrochar in terms of production,

420

physico-chemical properties and applications. Renew. Sust. Energy. Rev. 2015, 45, 359-378.

421

(15) Fuertes, A. B.; Arbestain, M. C.; Sevilla, M.; Maciá-Agulló, J. A.; Fiol, S.; López, R.; Smernik, R. J.;

422

Aitkenhead, W. P.; Arce, F.; Macías, F. Chemical and structural properties of carbonaceous products obtained by

423

pyrolysis and hydrothermal carbonisation of corn stover. Soil Res. 2010, 48, 618-626.

424

(16) Zhu, X.; Qian, F.; Liu, Y.; Zhang, S.; Chen, J. Environmental performances of hydrochar-derived magnetic

425

carbon composite affected by its carbonaceous precursor. RSC Adv. 2015, 5, 60713-60722.

426

(17) Sun, Y.; Gao, B.; Yao, Y.; Fang, J.; Zhang, M.; Zhou, Y.; Chen, H.; Yang, L. Effects of feedstock type,

427

production method, and pyrolysis temperature on biochar and hydrochar properties. Chem. Eng. J. 2014, 240,

428

574-578.

429

(18) Garlapalli, R. K.; Wirth, B.; Reza, M. T. Pyrolysis of hydrochar from digestate: Effect of hydrothermal

430

carbonization and pyrolysis temperatures on pyrochar formation. Bioresour. Technol. 2016, 220, 168-174.

431

(19) Zhu, X.; Liu, Y.; Qian, F.; Zhou, C.; Zhang, S.; Chen, J. Role of hydrochar properties on the porosity of

432

hydrochar-based porous carbon for their sustainable application. ACS Sustain. Chem. Eng. 2015, 3, 833-840.

433

(20) Zhu, X.; Liu, Y.; Qian, F.; Zhang, S.; Chen, J. Investigation on the physical and chemical properties of

434

hydrochar and its derived pyrolysis char for their potential application: influence of hydrothermal carbonization

435

conditions. Energ. Fuel. 2015, 29, 5222-5230.

436

(21) Kambo, H. S.; Dutta, A. A comparative review of biochar and hydrochar in terms of production,

437

physico-chemical properties and applications. Renew. Sust. Energ. Rev. 2015, 45, 359-378.

438

(22) Buss, W.; Mašek, O. Mobile organic compounds in biochar – a potential source of contamination – phytotoxic

439

effects on cress seed (Lepidium sativum) germination. J. Environ. Manage. 2014, 137, 111-119.

440

(23) Rombolà, A. G.; Marisi, G.; Torri, C.; Fabbri, D.; Buscaroli, A.; Ghidotti, M.; Hornung, A. Relationships

441

between chemical characteristics and phytotoxicity of biochar from poultry litter pyrolysis. J. Agr. Food Chem. 2015,

442

63, 6660-6667.

443

(24) Smith, C. R.; Hatcher, P. G.; Kumar, S.; Lee, J. W. Investigation into the sources of biochar water-soluble

444

organic compounds and their potential toxicity on aquatic microorganisms. ACS Sustain. Chem. Eng. 2016, 4,

22 ACS Paragon Plus Environment

Page 22 of 32

Page 23 of 32

Environmental Science & Technology

445

2550-2558.

446

(25) Huggins, T. M.; Haeger, A.; Biffinger, J. C.; Ren, Z. J. Granular biochar compared with activated carbon for

447

wastewater treatment and resource recovery. Water Res. 2016, 94, 225-232.

448

(26) Smith, C. R.; Buzan, E. M.; Lee, J. W. Potential impact of biochar water-extractable substances on

449

environmental sustainability. ACS Sustain. Chem. Eng. 2013, 1, 118-126.

450

(27) Ghidotti, M.; Fabbri, D.; Mašek, O.; Mackay, C. L.; Montalti, M.; Hornung, A. Source and biological response

451

of biochar organic compounds released into water; relationships with bio-oil composition and carbonization degree.

452

Environ. Sci. Technol. 2017, 51, 6580-6589.

453

(28) Titirici, M.-M.; White, R. J.; Falco, C.; Sevilla, M. Black perspectives for a green future: hydrothermal carbons

454

for environment protection and energy storage. Energ. Environ. Sci. 2012, 5, 6796-6822.

455

(29) Sun, Y.; Gao, B.; Yao, Y.; Fang, J.; Zhang, M.; Zhou, Y.; Chen, H.; Yang, L. Effects of feedstock type,

456

production method, and pyrolysis temperature on biochar and hydrochar properties. Chem. Eng. J. 2014, 240,

457

574-578.

458

(30) Wiedner, K.; Rumpel, C.; Steiner, C.; Pozzi, A.; Maas, R.; Glaser, B. Chemical evaluation of chars produced by

459

thermochemical conversion (gasification, pyrolysis and hydrothermal carbonization) of agro-industrial biomass on a

460

commercial scale. Biomass Bioenerg. 2013, 59, 264-278.

461

(31) Huff, M. D.; Kumar, S.; Lee, J. W. Comparative analysis of pinewood, peanut shell, and bamboo biomass

462

derived biochars produced via hydrothermal conversion and pyrolysis. J. Environ. Manag. 2014, 146, 303-308.

463

(32) Lu, X.; Pellechia, P. J.; Flora, J. R. V.; Berge, N. D. Influence of reaction time and temperature on product

464

formation and characteristics associated with the hydrothermal carbonization of cellulose. Bioresour. Technol. 2013,

465

138, 180-190.

466

(33) Kambo, H. S.; Dutta, A. Comparative evaluation of torrefaction and hydrothermal carbonization of

467

lignocellulosic biomass for the production of solid biofuel. Energy. Convers. Manag. 2015, 105, 746-755.

468

(34) Wiedner, K.; Naisse, C.; Rumpel, C.; Pozzi, A.; Wieczorek, P.; Glaser, B. Chemical modification of biomass

469

residues during hydrothermal carbonization -what makes the difference, temperature or feedstock? Org. Geochem.

470

2013, 54, 91-100.

471

(35) Peterson, A. A.; Vogel, F.; Lachance, R. P.; Fröling, M.; Antal, J. M. J.; Tester, J. W. Thermochemical biofuel

23 ACS Paragon Plus Environment

Environmental Science & Technology

472

production in hydrothermal media: a review of sub- and supercritical water technologies. Energ. Environ. Sci. 2008,

473

1, 32-65.

474

(36) Xu, Q.; Qian, Q.; Quek, A.; Ai, N.; Zeng, G.; Wang, J. Hydrothermal carbonization of macroalgae and the

475

effects of experimental parameters on the properties of hydrochars. ACS Sustain. Chem. Eng. 2013, 1, 1092-1101.

476

(37) Zhou, X. F. Conversion of kraft lignin under hydrothermal conditions. Bioresour. Technol. 2014, 170, 583-586.

477

(38) Koch, B. P.; Witt, M.; Engbrodt, R.; Dittmar, T.; Kattner, G. Molecular formulae of marine and terrigenous

478

dissolved organic matter detected by electrospray ionization Fourier transform ion cyclotron resonance mass

479

spectrometry. Geochim. Cosmochim. Acta 2005, 69, 3299-3308.

480

(39) Minor, E. C.; Steinbring, C. J.; Longnecker, K.; Kujawinski, E. B. Characterization of dissolved organic matter

481

in Lake Superior and its watershed using ultrahigh resolution mass spectrometry. Org. Geochem. 2012, 43, 1-11.

482

(40) Hockaday, W. C.; Grannas, A. M.; Kim, S.; Hatcher, P. G. Direct molecular evidence for the degradation and

483

mobility of black carbon in soils from ultrahigh-resolution mass spectral analysis of dissolved organic matter from a

484

fire-impacted forest soil. Org. Geochem. 2006, 37, 501-510.

485

(41) Smith, C. R.; Sleighter, R. L.; Hatcher, P. G.; Lee, J. W. Molecular characterization of inhibiting biochar

486

water-extractable substances using electrospray ionization Fourier transform ion cyclotron resonance mass

487

spectrometry. Environ. Sci. Technol. 2013, 47, 13294-13302.

488

(42) Antony, R.; Grannas, A. M.; Willoughby, A. S.; Sleighter, R. L.; Thamban, M.; Hatcher, P. G. Origin and

489

sources of dissolved organic matter in snow on the east Antarctic ice sheet. Environ. Sci. Technol. 2014, 48,

490

6151-6159.

491

(43) Liu, P.; Xu, C.; Shi, Q.; Pan, N.; Zhang, Y.; Zhao, S.; Chung, K. H. Characterization of sulfide compounds in

492

petroleum: selective oxidation followed by positive-ion electrospray fourier transform ion cyclotron resonance mass

493

spectrometry. Anal. Chem. 2010, 82, 6601-6606.

494

(44) Stedmon, C. A.; Bro, R. Characterizing dissolved organic matter fluorescence with parallel factor analysis: a

495

tutorial. Limnol. Oceanog. Met. 2008, 6, 572-579.

496

(45) Harvey, O. R.; Kuo, L. J.; Zimmerman, A. R.; Louchouarn, P.; Amonette, J. E.; Herbert, B. E. An index-based

497

approach to assessing recalcitrance and soil carbon sequestration potential of engineered black carbons (biochars).

498

Environ. Sci. Technol. 2012, 46, 1415-1421.

24 ACS Paragon Plus Environment

Page 24 of 32

Page 25 of 32

Environmental Science & Technology

499

(46) Dong, C. Q.; Zhang, Z. F.; Lu, Q.; Yang, Y. P. Characteristics and mechanism study of analytical fast pyrolysis

500

of poplar wood. Energ. Convers. Manage. 2012, 57, 49-59.

501

(47) Nitsos, C. K.; Matis, K. A.; Triantafyllidis, K. S. Optimization of hydrothermal pretreatment of lignocellulosic

502

biomass in the bioethanol production process. ChemSusChem 2013, 6, 110-122.

503

(48) Lv, J. T.; Zhang, S. Z.; Wang, S. S.; Luo, L.; Cao, D.; Christie, P. Molecular-scale investigation with

504

ESI-FT-ICR-MS on fractionation of dissolved organic matter induced by adsorption on iron oxyhydroxides. Environ.

505

Sci. Technol. 2016, 50, 2328-2336.

506

(49) Sleighter, R. L.; Hatcher, P. G. The application of electrospray ionization coupled to ultrahigh resolution mass

507

spectrometry for the molecular characterization of natural organic matter. J. Mass Spectrom. 2007, 42, 559-574.

508

(50) Cawley, K. M.; Hohner, A. K.; Podgorski, D. C.; Cooper, W. T.; Korak, J. A.; Rosario-Ortiz, F. L. Molecular

509

and spectroscopic characterization of water extractable organic matter from thermally altered soils reveal insight into

510

disinfection byproduct precursors. Environ. Sci. Technol. 2017, 51, 771-779.

511

(51) Koch, B. P.; Dittmar, T. From mass to structure: an aromaticity index for high-resolution mass data of natural

512

organic matter. Rapid Comm. Mass Spectrom. 2006, 20, 926-932.

513

(52) Uchimiya, M.; Ohno, T.; He, Z. Pyrolysis temperature-dependent release of dissolved organic carbon from plant,

514

manure, and biorefinery wastes. J. Anal. Appl. Pyrol. 2013, 104, 84-94.

515

(53) Jamieson, T.; Sager, E.; Guéguen, C. Characterization of biochar-derived dissolved organic matter using UV–

516

visible absorption and excitation-emission fluorescence spectroscopies. Chemosphere 2014, 103, 197-204.

517

(54) Uchimiya, M.; Hiradate, S.; Antal, M. J. Influence of carbonization methods on the aromaticity of pyrogenic

518

dissolved organic carbon. Energ. Fuel. 2015, 29, 2503-2513.

519

(55) Buss, W.; Mašek, O.; Graham, M.; Wüst, D. Inherent organic compounds in biochar-their content, composition

520

and potential toxic effects. J. Environ. Manag. 2015, 156, 150-157.

521 522

25 ACS Paragon Plus Environment

Environmental Science & Technology

3.0

a

2.5 Dehydration

H/C

2.0

Demethanation Bamboo 180°C

1.5

210°C 240°C

1.0

270°C 300°C 330°C

0.5 0.0 0.00

0.25

0.50

0.75 1.00 O/C

1.25

-OH

Transmittance (arbitrary units)

Decarboxylation

Page 26 of 32

aliphatic CH2

330 °C

C=O

300 °C 270 °C 240 °C

1200

210 °C 550

180 °C 2915

2840

3415

1703 1603 1113 1030 1504, 1460

1.50 4000 3500 3000 2500 2000 1500 1000 500 Wavenumber (cm-1)

Aromatic C 100

b

C=C C=C C-O-C C-O

d 330 °C

c

300 °C

Mass (%)

80 60 40

270 °C

180 °C 210 °C 240 °C 270 °C 300 °C 330 °C

240 °C

210 °C

180 °C

20 100 200 300 400 500 600 700 800 200 Temperature (°C)

160

120 80 40 Chemical shift (ppm)

0

Figure 1. (a) Effects of production temperature on H/C and O/C atomic ratios for hydrochar samples in Van Krevelen diagram, (b) FTIR spectra, (c) thermograms plots, and (d) Solid-state 13

C CP/MAS NMR spectra of hydrochar samples derived from varied production temperature.

26 ACS Paragon Plus Environment

Environmental Science & Technology

330 °C

28

240 °C 270 °C

24

300 °C 210 °C

20

180 °C

16 2 0

500 °C (biochar)

0

Mass release (mg /g Char)

1.6 1.2

4

8

12 16 Time (h)

Phenols Organic acids Furans Sugars

20

DOM Released (mg C/g Char)

a

32

36 30 24

24

c

0.8 0.4 0.0

b

180 °C 210 °C 240 °C 270 °C 300 °C 330 °C

42

18 4

Total released phenols (mg/g char)

DOM Released (mg C/g Char)

Page 27 of 32

180 210 240 270 300 330 Hydrochar production temperature (°C)

5

6

7 pH

8

9

10

d

1.6

1.2

y=0.007x-1.06 R2=0.97

0.8

0.4 180 210 240 270 300 330 Hydrochar production temperature (°C)

Figure 2. (a) Release kinetics of DOM from biochar and hydrochar produced at different temperatures, (b) the effect of solution pH on the amount of DOM released from hydrochar (24 h release), (c) the effect of hydrochar production temperature on the amount of detailed organic compounds in DOM, and (d) the correlation between hydrochar production temperature and total phenols in DOM.

27 ACS Paragon Plus Environment

Environmental Science & Technology

2.0 Lipids

210 °C

240 °C

CHO: 777

CHO: 847

270 °C

300 °C

330 °C

CHO: 929

CHO: 866

CHO: 651

Proteins/amino sugars 180 °C

1.0

Unsaturated hydrocarbons

H/C

CHO: 772

1.5

Lignins

Condensed aromatics

0.5 2.0

H/C

Page 28 of 32

1.5

1.0

0.5 0.0

0.2

0.4 O/C

0.6

0.8

0.2

0.4 O/C

0.6

0.8

0.2

0.4 O/C

0.6

0.8

Figure 3.Van Krevelen plots for CHO molecular formulas assigned to the ESI FT-ICR MS spectral peaks in different HTL temperature derived hydrochar-based DOM. The size of sphere represents the relative abundance of one type of molecular formula. The CHO number represents the numbers of CHO molecules in DOM. Boxes overlain on the plots indicate biomolecular compound classes.

28 ACS Paragon Plus Environment

Page 29 of 32

Environmental Science & Technology

a

28

Aliphatic Aromatic Condensed Aromatic

60

40

b 330 °C

300 °C

270 °C

240 °C

210 °C

180 °C

24

Percentage (%)

Relative abundance (%)

80

20

20 16 12 8 4

0 180

210

240

270

300

0

330

O2 O3 O4 O5 O6 O7 O8 O9 O10 Ox Class Species

Hydrochar production temperature (°C)

O

d 7

HO O

c

330 °C

300 °C

270 °C

240 °C

210 °C

C15H30O2

HO

180 °C

O

6

C17H34O2

HO

330 °C

5

DBE = 1

Percentage (%)

C14H28O2

4

300 °C 270 °C

3

240 °C

2

210 °C

1 180 °C

0 16

N1O3 N1O4 N1O5 N O N1O7 N1O8 1 6 N1Ox Class Species

20 24 Carbon Number

28

Figure 4. (a) Aliphatic and aromatic compounds distribution in DOM from hydrochar produced at different temperatures. Formulas are classified as aliphatic (AImod < 0.5), aromatic (0.5 < AImod < 0.67), and condensed aromatic (AImod > 0.67). AImod (aromaticity index) was calculated based on reference 45. (b) Ox and (c) N1Ox species distribution in different hydrochar-based DOM. (d) DBE (double-bond equivalent) versus carbon number distribution of possible organic acids in different hydrochar-based DOM detected by ESI FT -ICR MS. The sphere size represents the relative abundance of one type of molecular formula.

29 ACS Paragon Plus Environment

Environmental Science & Technology

I

Page 30 of 32

II

III

IV

Figure 5. Fingerprint EEM from 4 component PARAFAC (parallel factor) modeling of hyrochar-based DOM.

30 ACS Paragon Plus Environment

Page 31 of 32

Environmental Science & Technology

1.2

a Biochar

100

Inhibition Rate (%)

Absorbance

Hydrochar

120

1.0 0.8

80 60 40 20

0.6

0

180 210 240 270 300 330 500 Temperature (°C)

0.4 180 °C 210 °C 240 °C 270 °C

0.2

300 °C 330 °C blank 500 °C

0.0

Cyanobacteria inhibition rate (%)

0

1

2

3 4 5 Time ( Day)

c

100

6

7

330 °C

80 60 y=24.6x-23.1

40

R2=0.95 240 °C

20

210 °C

300 °C

270 °C

0 180 °C

-20 0

1 2 3 4 Percentage of N1O4 class (%)

5

Figure 6. (a) Cyanobacterial growth treated with DOM from hydrochar produced at different temperatures (the blank represents the cyanobacteria treated with 25 mL of pure water), (b) the microphotos of cyanobacterial growth with different hydrochar-based DOM, the cyanobacterial growth can be estimated by the depth of green and the size of cyanobacterial cluster and (c) the correlation between cyanobacterial inhibition rate and percentage of N1O4 class chemicals of different hydrochar-based DOM.

31 ACS Paragon Plus Environment

Environmental Science & Technology

Table of Contents

Hydrochar-DOM Influence Algae Growth 180 oC 240 oC 270 oC 330 oC +4.0% -13.1% -15.3% 180 oC

330 oC

Molecules Changes -98.7%

ACS Paragon Plus Environment

Page 32 of 32