Biochemistry 1994, 33, 8833-8841
8833
Reductive Half-Reaction of Medium-Chain Fatty Acyl-CoA Dehydrogenase Utilizing Octanoyl-CoA/Octenoyl-CoA as a Physiological Substrate/Product Pair: Similarity in the Microscopic Pathways of Octanoyl-CoA Oxidation and Octenoyl-CoA Binding? N. Ravi Kumar and D. K. Srivastava' Biochemistry Department, North Dakota State University, Fargo, North Dakota 58105 Received April 6, 1994; Revised Manuscript Received May 19, 1994"
ABSTRACT: Of the different chain length fatty acyl-CoA substrates, octanoyl-CoA has been known as one
of the most efficient (and physiological) substrates for the medium-chain fatty acyl-CoA dehydrogenase (MCAD)-catalyzed reaction. The reaction of MCAD-FAD with octanoyl-CoA ([MCAD-FAD]
