Relationship between Molecular Flexibility and Emulsifying Properties

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Functional Structure/Activity Relationships

Relationship between Molecular Flexibility and Emulsifying Properties of Soy Protein Isolate-Glucose Conjugates Rui Li, Xibo Wang, Jingnan Liu, Qiang Cui, Xiaodan Wang, Shuang Chen, and Jiang Lianzhou J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b06713 • Publication Date (Web): 18 Mar 2019 Downloaded from http://pubs.acs.org on March 19, 2019

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Journal of Agricultural and Food Chemistry

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Relationship between Molecular Flexibility and Emulsifying

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Properties of Soy Protein Isolate-Glucose Conjugates

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Rui Li, Xibo Wang*, Jingnan Liu, Qiang Cui, Xiaodan Wang, Shuang Chen, Lianzhou

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Jiang

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College of Food Science, Northeast Agricultural University, Harbin 150030, China

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*Corresponding Author. Tel.: +86-15945155869.

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E-mail addresses:[email protected] (Rui Li); [email protected] (Xibo

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Wang*); [email protected] (Jingnan Liu); [email protected] (Qiang Cui);

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[email protected] (Xiaodan Wang); [email protected] (Shuang Chen);

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[email protected] (Lianzhou Jiang)

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Postal address: College of Food Science, Northeast Agricultural University, Harbin

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150030, Heilongjiang, China.

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ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

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Abstract

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At present, the structure-activity relationships of soy protein isolate are still not

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well understood. In this paper, the relationship between molecular flexibility and

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emulsifying properties of soy protein isolate and soy protein isolate-glucose conjugates

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were investigated. The Maillard reaction was carried out at different temperature

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conditions (50 ℃, 60 ℃, 70 ℃, 80 ℃ and 90 ℃, respectively) under a specific wet

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condition. Meanwhile, structural properties including surface hydrophobicity (H0),

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molecular flexibility and secondary, tertiary, quaternary structures and the free

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sulfhydryl group (-SH) contents were measured. The results showed that there was a

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good correlation between molecular flexibility and emulsifying properties, and the

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correlation coefficients was 0.920 (P < 0.01) for emulsifying activity and 0.952 (P