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No-fuss quantitative proteomics with pseudo-internal standards MS may be the defining 58–70% for PIS compared Calculate the ratio of ratios Generate LC/MS data for controls and for for the peak combinations. experimental samples. tool of proteomics, but it is with 88–97% for ion (1:2) / (1:2) = 2.1 far from quantitative. Difintensity data; precision (1:3) / (1:3) = 1.0 ferences in sample preparawith stable-isotope-based (1:4) / (1:4) = 1.0 (1:5) / (1:5) = 1.0 tion, instrument calibration, techniques, by compari(2:3) / (2:3) = 0.6 1 2 3 4 5 1 2 3 4 5 ionization efficiencies, and son, was typically
