SCIENCE & TECHNOLOGY
SPEEDING UP MASS SPEC IMAGING Detector borrowed from high-energy physics makes it easy to create DETAILED IMAGES of tiny samples CELIA HENRY ARNAUD, C&EN WASHINGTON
A PICTURE SAYS more than words alone.
MS imaging to peer at a part of the fruit fly larva called the imaginal disc, which develops into the adult fly’s wing. “In the development of Drosophila wings, there are particular signaling molecules that we would like to local-
COURTESY OF CASIMIR BAMBERGER
That maxim is just as true for chemical images as it is for paintings or other illustrations. Mass spectrometric (MS) imaging reveals not only the chemical makeup of a sample but also the spatial distribution of species. Conventional MS imaging draws chemical pictures dot-by-dot, much like a pointillist painting. In matrixassisted laser desorption/ionization (MALDI) MS imaging, the most common type of MS imaging, a laser beam is moved laboriously from point to point, so that at each point the MS instrument can collect spectral information about species on the sample surface. If spectral acquisition takes a second per spot, then a 100 × 100 array takes nearly three hours to complete. Mass spectrometrists trying to speed up this process have found one potential tool in the kit of highenergy physicists. A new detector, called the Timepix, allows researchers to acquire both the location and mass of ions in a 200-µm-diameter area on the surface. The Timepix is particularly useful for MS imaging of very small samples where detailed information is needed, says Ron M. A. Heeren, a group leader at the Foundation for Fundamental Research on Matter (FOM) Institute for Atomic & Molecular Physics, in Amsterdam. Heeren and the father-and-son team of Andreas Bamberger, a high-energy physicist at the University of Freiburg, Germany, and Casimir Bamberger, a researcher at Scripps Research Institute, are independently pioneering the application of this detector to MS imaging. In a prime example of the new detector’s power, Heeren’s group has collected images that reveal tiny features on fruit fly larvae. Heeren is using the Timepix and
The best spatial resolution a user can typically hope for with conventional MS imaging is about 100 µm. To show they can do better with the Timepix, the Bambergers and their colleague Uwe Renz collected digital MS images from a simple homebuilt time-of-flight mass spectrometer coupled with the detector (J. Am. Soc. Mass Spectrom., DOI: 10.1007/s13361-011-0120-1). They achieved spatial resolution of about 85 µm over a sample area of about 2 cm2, approximately the area covered by a standard histology tissue section. Heeren and coworkers have pushed spatial resolution to 3.4 µm with the help of commercial mass spectrometers with high-quality ion optics and magnification properties that give better spatial resolution and wider mass range (Anal. Chem., DOI: 10.1021/ac2017629). It may be possible to push spatial resolution into the nanometer range, Heeren says. But getting down to single nanometers won’t be easy and may not even be possible. “How many intact biomolecules will you actually desorb and ionize on a square nanometer? There aren’t going to be many,” he says. THE TIMEPIX was developed at
CERN, the European Organization for Nuclear Research, by the Medipix collaboration with support from the EUDET collaboration, both of which are projects to develop detectors for highenergy physics. Many organizations participated in the development, including the University of Freiburg and FOM. Physicists sought to develop a detector that would let them track the position of high-energy particles. That detector consists of ize on the surface of these DETECTOR The Timepix, more than 65,000 individually which is 14 mm wide, programmable pixels. In one discs,” Heeren says. With consists of more than mode, a pixel counts the number conventional MS imaging, 65,000 individually of “clock cycles” between the la“we would use a long time programmable pixels. ser pulse and the ion’s arrival at to image those and not get the detector. Because all ions are the spatial detail we’re interaccelerated to the same kinetic ested in. With this approach, energy, that flight time can be used to calcuwe can do the entire wing disc rapidly with late the ion’s mass. In another mode, pixels 500-nm pixels.” They acquire an entire imcan measure signal intensity by determining age in about 30 minutes, 10 times as fast as the length of time the charge is detectable. using standard detectors. Pixels in these two modes can be arranged Beyond speed, images obtained by this in different configurations on the Timepix. approach have another advantage over conRather than measure ions directly, ventional MS scanning images—resolution. WWW.CEN-ONLINE.ORG
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has been pleasantly surprised by the mass range that he can achieve with of the peptide angiotensin on a surface was the Timepix. He was recently able to obtained with each laser shot using the Timepix acquire an MS image of a large, 78-kidetector. Each pixel is 55 µm wide. The intensity is lodalton, complex containing nine the integration of images from 1,000 laser shots. ubiquitin proteins. His team was able to acquire these mass spectra at an acceleration energy of only 3 kiloelectron volts (keV). Most commercial MALDI mass spectrometers require acceleration energies of 25 keV to achieve similar mass spectra. Heeren and his team are already planning to use higher acceleration energies to improve sensitivity for high-mass ions. “We expect that we’ll be able to extend it to even higher masses,” he says. Heeren’s group has received a $1.3 million grant from the Dutch Technology Foundation to further develop the Timepix for MS imaging and for color X-ray imaging. As part at least 10-fold too slow,” Casimir Bamof that work, Heeren plans to build more berger says. He hopes the next generation of these detectors and take them on the of the chip will be at least 10 times as fast. road to scientists who are interested in usEven with those limitations, Heeren ing them. ◾
I SPY An MS image of a grid on top of a solution
ADAPTED FROM A NA L. C HEM.
Heeren and the Bambergers both place a multichannel plate in front of the Timepix. When ions strike it, the multichannel plate generates an electron shower that the Timepix detects. Because of the signal amplification possible with the multichannel plate, the setup is more sensitive than the chip alone and can detect a wider concentration range of ions. The Timepix captures a distinct ion mass every 10 to 20 nanoseconds, which corresponds to one clock cycle. However, because at most 10,000 clock cycles can be stored, only a limited mass range can be collected per laser shot. More masses can be collected by waiting before triggering the detector’s clock and then stitching the mass spectrum together. The clock speed determines the difference between distinct ion masses and thus the achievable mass resolution. The clock speed—about 100 MHz—is still slow and thus the mass resolution is low. “If you compare that to the clocks used in standard mass spectrometers, it’s
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