Structure and dynamics of a glyceroglycolipid - ACS Publications

Mar 6, 1986 - and rotated so that in the new axis system the origin was at. C5', the ..... Cells (Hynes, R. O., Ed.) Chapter 3, Wiley, New York. Davis...
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Biochemistry 1986,25, 3950-3957

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Lienhard, G. E.(1983)Trends Biochem. Sci. (Pers. Ed.) 8,

125-1 28. Lowry, 0 .H. Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951) J . Biol. Chem. 193,265-275. Lucy, J. A. (1984)FEBS Lett. 166,223-231. Morihara, K. (1974)Adv. Enzymol. Relat. Areas Mol. Biol. 41, 179-243. Mumford, R. A., Strauss, A. W., Powers, J. C., Pierzchala, P. A., Nishino, N., & Zimmerman, M. (1980) J . Biol. Chem. 255, 2227-2230. Mundy, D. I., & Strittmatter, W. J. (1985) Cell (Cambridge, Mass.) 40, 645-656. Orlowski, M., & Wilk, S.(1981)Biochemistry 20,4942-4950. Pilch, P. F.,Thompson, P. A., & Czech, M. P. (1980)Proc. Natl. Acad. Sci. U.S.A. 77, 915-918. Rodbell, M. (1964)J . Biol. Chem. 239, 375-380. Scheid, A.,& Choppin, P. W. (1974) Virology 57 475-490.

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327-331. Silverstein, S . C., Steinman, R. S. M., & Cohn, Z.(1977) Annu. Rev. Biochem. 46, 669-122. Simpson, I. A., Yver, D. R., Hissin, P. J., Wardzala, L. J., Karnielli, E., Salans, L. B., & Cushman, S. W. (1983) Biochim. Biophys. Acta 763, 393-407. Suzuki, K., & Kono, T. (1980)Proc. Natl. Acad. Sci. U.S.A.

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Structure and Dynamics of a Glyceroglycolipid: A 2H NMR Study of Head Group Orientation, Ordering, and Effect on Lipid Aggregate Structure? Harold C. Jarrell,* Jerzy B. Giziewicz, and Ian C. P. Smith Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1 A OR6 Received November 5. 1985; Revised Manuscript Received March 6, 1986

ABSTRACT: The head group orientation and the motional characteristics of

1,2-di-O-tetradecy1-3-O-P-~-

glucopyranosylglycerol selectively 2H-labeled on the glucose moiety have been investigated by differential scanning calorimetry and 2H N M R . The glycolipid undergoes a major endothermic transition a t 52 O C , which is attributed to the gel to liquid-crystal phase transition. The nature of a less energetic endothermic transition a t 58 OC,determined to be a lamellar to hexagonal mesophase transition by 2H N M R , is confirmed by X-ray diffraction. In the lamellar phase, the glycolipid head group undergoes axially symmetric motion and has an orientational order parameter Smol of 0.45, which is significantly larger than that (0.3 1) reported for an analogous glucosylcerebroside. The head group is extended away from the bilayer surface. On entering the hexagonal mesophase, the orientational order parameter for the sugar ring is reduced slightly to 0.38, but the local rotation axis undergoes a large reorientation with respect to the carbohydrate ring. In a phospholipid matrix, the orientation of the carbohydrate head group of the glycolipid is affected by the greater extension of the surface residues of the host lipid. Two orientations of the exocyclic hydroxyl group of the carbohydrate moiety were detected by *H N M R and are shown to have unequal populations.

Glycolipids constitute a class of lipid that occurs in plants, microorganisms, and animals (Gigg, 1980). Glycolipids are most frequently composed of a carbohydrate head group anchored to the membrane through a diacyl- (or dialkyl-) glycerol or a sphingosine residue. The carbohydrate head group can be relatively simple (e.g., a single sugar' residue) or very complex and may be neutral or charged (Gigg, 1980). Carbohydrates at cell surfaces have been implicated in important cellular events such as cell-cell recognition, ligand-receptor interaction [e.g., cholera toxin receptor (Critchley, 1979)],and ion transport (Karlsson, 1977). The involvement of carbohydrates in such biologically important functions is dependent upon the primary sequence of the surface component but most certainly is also dependent upon the spatial relationship of the constituent residues. Glycolipids may also be the major constituent lipid of membranes such as in Acholeplasma 'Issued as NRCC Publication No. 25827.

0006-2960/86/0425-3950$01.50/0

laidlawii (Rottem, 1980;Razin, 1978) and in such cases must play a major role in defining the physical properties of the cellular membrane. In view of the major but diverse roles that glycolipids can assume, the elucidation of the orientation of the carbohydrate residues relative to the membrane surface and the dynamical behavior of the head group are of considerable interest. In addition, the response of the head group orientation and motion to perturbations such as ion binding, ligand-receptor interactions, and other surface components is of fundamental importance to the understanding of cell surface phenomena. Deuterium (2H) NMR' is a powerful technique for the elucidation of orientational and motional properties of mole] Abbreviations: PC, phosphatidylcholine; PE, phosphatidylethanolamine; DTGL, 1,2-di-O-tetradecyl-3-O-~-D-glucopyranosyl-rac-glycerol; GC, glucocerebroside; DSC, differential scanning calorimetry; NMR, nuclear magnetic resonance; TLC, thin-layer chromatography.

0 1986 American Chemical Society

GLYCEROGLYCOLIPID STRUCTURE AND DYNAMICS

cules in an anisotropic environment such as that found for lipid molecules in a membrane (Seelig, 1977; Davis, 1983; Smith, 1984). To date, only one detailed 2H NMR study of the head group properties of a glycolipid, N-palmitoylglucosylceramide (GC), in aqueous multilamellar dispersions has been reported (Skarjune & Oldfield, 1982). This study investigates the head group region of a glyceroglycolipid, namely, 1,2-di-O-tetradecyl-3-O-P-~-glucopyranosylglycerol. A dialkylgiycerol lipid was selected for several reasons. First, the attachment of the long lipophilic chains to glycerol by an ether linkage is stable compared to the usual ester bond, and thus, further chemical elaboration of the head group to oligosaccharide moieties is facilitated. The dialkylglycerol moiety would therefore serve as a stable lipophilic group, which could be used to anchor more complex head groups to the membrane. Second, the glycolipid provides a model of the dialkylglycolipids of Halobacterium cutirubrum (Kates, 1978). Third, although the replacement of fatty acyl chains with the corresponding alkyl chains in phospholipids does lead to some differences in the physical properties (e.g., T,) of the lipid, these changes do not appear to be dramatic (Dorset & Pangborn, 1982; Harlos & Eibl, 1980; Eibl & Blume, 1979). This study establishes that the head group of the glycolipid 1,2-di-O-tetradecyl-3-0-/3D-glucopyranosylglycerolin aqueous multilamellar dispersions undergoes rapid axially symmetric motion, exhibits a lamellar-hexagonal structural transition, and is extended away from the bilayer surface. In addition, two orientations of the exocyclic hydroxyl group, which are in slow exchange on the 2H NMR time scale, are detected.

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prepared by dissolving DMPC and DTGL in chloroformmethanol (l:l), removing the organic solvent with nitrogen gas, and lyophilizing the residue from water. Hydrated samples were cyclically heated to 60 OC with vortex mixing and freeze-thawed to homogeneity (four to five cycles). 2H NMR spectra were obtained at 30.7 MHz on a "home-built" Fourier-transform spectrometer. Experiment control and data collection were performed with a Nicolet 1280 computer, a 293B pulse programmer, and an NIC-2090 digital oscilloscope (Nicolet Instrument Corp., Madison, WI). The observe frequency was generated with a PTS 160 frequency synthesizer and by a Varian 4420 amplifier driven by the output of an ENI-3100L amplifier. Data were stored on a Control Data 9427 H disc system. Spectra were acquired by quadrature detection and the quadrupolar echo sequence (Davis et al., 1976) with full-phase cycling of the radio-frequency pulses. Typically, spectra were obtained with a 90" pulse width of 4-5 ps (10-mm solenoid coil), a 60-ps delay between the 7r/2 pulses, and a recycle time of 100 ms. The frequency of the spectrometer was carefully set at the center of the quadrupolar powder pattern. Relaxation times, Tlrr were measured by the inversion-recovery procedure in combination with the quadrupolar echo sequence as described elsewhere (Perly et al., 1984) and were