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Agricultural and Environmental Chemistry
Tryptophan supplementation increases reproduction performance, milk yield and milk composition in lactating sows and production performance of their piglets Jinfeng Miao, Deborah Adewole, Shangxi Liu, Panpan Xi, Chengbo Yang, and Yulong Yin J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.9b00446 • Publication Date (Web): 22 Apr 2019 Downloaded from http://pubs.acs.org on April 22, 2019
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Journal of Agricultural and Food Chemistry
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Tryptophan supplementation increases reproduction performance, milk yield and milk composition in lactating sows and growth performance of their piglets Jinfeng Miao,†, ‡ Deborah Adewole,§, ⊥ Shangxi Liu,⊥ Panpan Xi,‡ Chengbo Yang⊥ and Yulong Yin†* †Key
Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical
Agriculture, Chinese Academy of Sciences; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan, 410125, P.R. China ‡MOE
Joint International Research Laboratory of Animal Health and Food Safety, College of
Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China §Department
of Animal Science and Aquaculture, Dalhousie University, Truro, Nova Scotia B2N
5E3, Canada ⊥Department
of Animal Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
*Corresponding author Fax: +86 731 84612685. E-mail address:
[email protected] ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Tryptophan (Trp) can produce bioactive compounds for appetite regulation, calcium mobilization,
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and mammary gland homeostasis via a serotonin pathway. This study evaluated the effects of Trp
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supplementation on the reproduction performance, milk yield and composition of lactating sows,
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growth performance of their piglets and the secretion function of porcine mammary epithelial cells
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(PMECs). The infrared emulsion analyzer and ELISA analyses revealed that feeding sows with a
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0.12% Trp addition increased (P < 0.05) sow average daily feed intake, milk yield, milk calcium
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concentration, average daily gain of piglets, fatty acid synthase (FAS) and lactose synthase (LS),
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β-casein secretion, intracellular Ca2+ level, the expression of calcium binding protein CaM and the
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activity of CaMKII. In a cellular experiment of PMECs treated with Trp, ELISA and Flow
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cytometry analyses revealed that the pre-treatment of a Trp hydroxylase inhibitor reduced (P
10 piglets and placing them with sows of the same group having
