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Biofuels and Biomass
Variations in microbiota communities with the ranks of coals from three Permian mining areas Bingjun Liu, Liang Yuan, Xianyang Shi, Yang Li, Chunlu Jiang, Bo Ren, and Qingye Sun Energy Fuels, Just Accepted Manuscript • DOI: 10.1021/acs.energyfuels.8b04413 • Publication Date (Web): 29 Apr 2019 Downloaded from http://pubs.acs.org on April 29, 2019
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Energy & Fuels
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Variations in microbiota communities with the ranks of coals from three Permian mining areas
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Bingjun Liu,1,2 Liang Yuan, *,1 Xianyang Shi,1 Yang Li,1 Chunlu Jiang,1 Bo Ren2, Qingye
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Sun1
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230601, People’s Republic of China
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232000, People’s Republic of China
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School of Resources and Environmental Engineering, Anhui University, Hefei, Anhui Coal Mining National Engineering Technology Research Institute, Huainna, Anhui
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ABSTRACT: Microorganisms play vital roles in the biogeochemical processes, such as biomethane production, which is an important source of coalbed methane (CBM), that occur in coalbeds. However, little information is available regarding the microbial communities in in situ coalbeds of different ranks from Permian mining areas. In this study, anaerobic Biolog ECO microplates were used to determine the microbial metabolic activity, and high-throughput sequencing was used to investigate the bacteria and archaea communities in three typical mining areas. Anaerobic Biolog ECO microplates showed that the peak value of average well color development (AWCD) in low-rank coal seams from Anhui Province (A-L) was 0.46 higher than that of medium-rank coal from Shanxi Province (S-M). Phylogenetic analysis indicated that the bacterial phyla Proteobacteria, Firmicutes and Actinobacteria were the predominant lineages in all samples; in particular, the family Oxalobacteraceae accounted for the overwhelming majority of the bacterial sequences (from 47.20% to 92.67%). Most of the identified genera, Herbaspirillum, Pseudomonas and Acinetobacter, were fermentative bacteria that can convert coal macromolecules into methanol, acetic acid and CO2. In addition, the archaea in A-L were dominated by the phyla Euryarchaeota, Thaumarchaeota and Woesearchaeota. Additionally, Euryarchaeota was the dominant archaeal lineage in S-M, and Thaumarchaeota was predominant in the Guizhou Province group of high-rank coals (G-H). Canonical correspondence analysis (CCA) showed that there were significant correlations between the microbial community and porosity, hydrogen content and oxygen content (p
