BRIEFS
Polar-Liquid, Derivatized Cyclodextrin Stationary Phases for the Capillary Gas Chromatography Separation of Enantiomers 914
Perspective: Analytical Biotechnology New Developments in Biochemical Mass Spectrometry: Electrospray Ionization
882
The principles, development, and recent application of elec trospray ionization mass spectrometry to biological com pounds are reviewed. Richard D, Smith*, Joseph A. Loo, Charles G. Edmonds, Charles J. Barinaga, and Harold R. Udseth, Chemical Methods and Separations Group, Chemical Sciences Department, Pacific Northwest Laboratory, Richland, WA 99352
Hydrophilic permethyl 0-(S)-2-hydroxypropyl derivatives of α-, β-, and 7-cyclodextrins are used as GC stationary phases for the separation of enantiomeric solutes. Properties of these cyclodextrin derivatives are examined, and the sep aration mechanism is considered. Daniel W. Armstrong*, Weiyong Li, and Chau-Dung Chang, Department of Chemistry, University of Missouri—Rolla, Rolla, MO 65401 and Josef Pitha, National Institutes of Health, NIA/ GRC, Baltimore, MD 21224
Articles
Theoretical Study of System Peaks in Linear Chromatography 923
High-Speed Separations of DNA Sequencing Reactions by Capillary Electrophoresis 900
When the sample components give no detector response and a retained additive in the mobile phase does give a detector response, the theory of system peaks in LC makes it possible to predict response factors. Sadroddin Golshan-Shirazi and Georges Guiochon*, Depart ment of Chemistry, University of Tennessee, Knoxville, TN 379961600, and Division of Analytical Chemistry, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6120
Pluorescently labeled DNA fragments, generated in enzy matic sequencing reactions, are separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. Howard Drossman, John A. Luckey, Anthony J. Kostichka, Jonathan D'Cunha, and Lloyd M. Smith*, Department of Chem istry, University of Wisconsin, Madison WI 53706 Theory for Cyclic Staircase Voltammetry for First-Order Coupled Reactions
903
Working curves are presented for the response in cyclic staircase voltammetry for first-order preceding, following, and catalytic homogeneous reactions coupled to reversible charge transfer. Mary Margaret Murphy, John J. O'Dea, Dieter Arn, and Janet G. Osteryoung*, Department of Chemistry, SUNY University at Buffalo, Buffalo, NY 14214 Conductive Polymeric Tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin Film Electrode for Trace Determination of Nickel 909 Stable conductive polymer film electrodes are formed from tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin with Ni(II) as the central metal. The demetalated film exhibits a strong affinity for Ni(II). Tadeusz Malinski*, Aleksander Ciszewski, and Judith R. Fish, Department of Chemistry, Oakland University, Rochester, MI 48309-4401 and Leszek Czuchajowski*, Department of Chemis try, University of Idaho, Moscow, ID 83843
* Corresponding author 520 A · ANALYTICAL CHEMISTRY, VOL. 62, NO. 9, MAY 1, 1990
Flow System for Direct Determination of Enzyme Substrate in Undiluted Whole Blood 932 Enzyme sensors using a reagent and controlled pH that detect substrate at non-steady-state conditions are de scribed. Hematocrit dependence is minimized by using seg mented sample injections, membrane-deposited reagents, and membranes with low permeability. T. Buch-Rasmussen, Radiometer Medical A/S, Emdrupvej 72, DK-2400 Copenhagen, Denmark Design and Synthesis of Lithium lonophores for an IonSelective Electrode by Chemical Modification of Natural Carboxylic Polyether Antibiotic Monensin +
936
In order to design Li -selective ionophores for an ion-selec tive electrode, the relationship between the ion selectivities of electrodes based on 12 derivatives of the natural antibiotic monensin and those derivatives' chemical structures is in vestigated. Koji Tohda, Koji Suzuki*, Nobutaka Kosuge, Kazuhiko Watanabe, Hitoshi Nagashima, Hidenari Inoue, and Tsuneo Shirai, Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223, Japan
MAXIMIZE YOUR SENSITIVITY... for Volatile Organic Compounds by Dynamic Headspace Concentration
BRIEFS
Determination of Copper in Seawater by Anodic Stripping Voltammetry Using Ethylenediamine
943
Differential pulse anodic stripping voltammetry using a hanging drop mercury electrode is used to detect Cu in seawater at a level of 5 Χ 10 - 1 0 M. A procedure for measuring pH 8 labile and ligand-exchangeable Cu is presented. Gioacchino Scarano, Elisabetta Morelli, Alfredo Seritti, and Alberto Zirino*, Istituto di Biofisica, Consiglio Nazionale delle Ricerche, via S. Lorenzo 26, Pisa 56100, Italy Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis 949 Electron spectroscopy for chemical analysis is used to eluci date the chemical composition and organic functional group distribution within one type of biopolymer, eumelanin. Michael B. Clark, Jr., Joseph A. Gardella, Jr.*, Thomas M. Schultz, Dilip G. Patil, and Lawrence Salvati, Jr., Department of Chemistry and Surface Science Center, State University of New York at Buffalo, Buffalo, NY 14214, Clairol Research Laboratories, 2 Blachley Road, Stamford, CT 06902, and Perkin-Elmer Physical Electronics Laboratories, 5 Progress Street, Edison, NJ 08820 Investigations of the Electrospray Interface for Liquid Chromatography/Mass Spectrometry
957
Factors affecting the yield of positive ions in the gas phase from electrosprayed methanol solutions of some inorganic ions and 30 organic onium ions B H + from nitrogen bases Β are studied with an atmospheric pressure triple quadrupole mass spectrometer. Michael G. Ikonomou, Arthur T. Blades, and Paul Kebarle*, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada Preparation and Characteristics of a Crown Ether Polysiloxane Stationary Phase for Capillary Gas Chromatography 968 5 ml Coffee on Tekmar's LSC 2000 and Capillary Interface
Flavor/Fragrance Competitive Analysis Off Flavor/Odor Analysis Packaging Materials Pharmaceuticals/Residual Solvents Building Products/Outgassing Studies Polymers Residual Monomers/Solvents Ask for our FREE bibliography of reprints on a wide range of applications V)NL*ll}&i*il3?iililiil?fcït
lllJillfifA
Tekmar
®
P.O. Box 371856 · Cincinnati, OH 45222-1856 (800) 543-4461 Sales · (800) 874-2004 Service Fax (513) 761 -5183 «Telex 21 -4221 CIRCLE 135 ON READER SERVICE CARD 522 A · ANALYTICAL CHEMISTRY, VOL. 62, NO. 9, MAY 1, 1990
Chromatographic characteristics, including column efficien cy, allowable temperature range, thermal stability, polarity, and selectivity, are studied. The stationary phase is similar to Carbowax-20M in polarity and selectivity and has an operational temperature range of 70-300 °C. Cai-Ying W u \ Cheng-Ming Wang, Zhao-Rui Zeng, and XueRan Lu, Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China
Technical Notes Gas Chromatographic Separation of Carbon Dioxide and Nitrous Oxide for Stable Isotopic Analysis of Carbon Dioxide 972 Kinga Revesz* and Tyler B. Coplen, U.S. Geological Survey, 431 National Center, Reston, VA 22092 Very High Detectability in Two-Photon Spectroscopy 973 M. J. Wirth* and H. O. Fatunmbi, Department of Chemistry & Biochemistry, University of Delaware, Newark, DE 19716
Correction. Determination of Serum Cholesterol by a Modification of the Isotope Dilution Mass Spectrometric Definitive Method 976 Polly Ellerbe*, Stanley Meiselman, Lorna T. Sniegoski, Mi chael J. Welch, and Edward White V, Center for Analytical Chemistry, National Institute of Standards and Technology, Gaithersburg, MD 20899
