Digital Analysis of Electronic Absorption Spectre - Analytical Chemistry

May 24, 2012 - U Sharma , J.C Marquis , A Nicole Dinaut , S.M Hillier , B Fedeles , P.T Rye , J.M Essigmann , R.G Croy. Bioorganic & Medicinal Chemist...
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digital Analysis cf

David E. Metzler Carol M. Harris Department of Biochemistry and Biophysics Iowa State University Ames, Iowa 50011

Richard L. Reeves 1 W. H. Lawton Mary S. Maggio Eastman Kodak Co., 1669 Lake Avenue Rochester, N.Y. 14650

The wide use of spectrophotometry in chemical analysis results from the fact that electronic spectra of substances in solution are easy to measure with high precision and at relatively low concentrations. Despite the ease of measurement, a large amount of the information contained in the original spectra is seldom used in analyses and is almost never reported. As a result, the conventional use of data at a few wavelengths requires relatively large spectral changes for a given precision, whereas use of the information in the entire absorption curve would allow small changes to be analyzed with the same precision. Attempts at empirical correlations are made difficult by the fact that the literature contains the scantiest of data: