162
Bioconjugate Chem., Vol. 1, No. 2, 1990
Instructions to Authors for Bioconjugate Chemistry 1. Forwarding Address. Manuscripts for publication in Bioconjugate Chemistry should be submitted in triplicate to Claude F. Meares, Editor, Department of Chemistry, Room 190A, University of California, Davis, CA 95616. Include a signed ACS copyright status form, a copy of which may be found in the first issue of the year of any American Chemical Society journal. Correspondence regarding accepted papers, proofs, and reprints should be directed to Journals Department, American Chemical Society, 2540 Olentangy River Road, P.O. Box 3330, Columbus, OH 43210. 2. Scope and Editorial Policies. Bioconjugate Chemistry invites original contributions on all aspects of the joining of two molecular functions by chemical or biological means. This includes, among other topics, the conjugation of antibodies, nucleic acids, lipids, carbohydrates, or other biologically active molecules with each other or with any molecular groups that add useful properties (drugs, radionuclides, toxins, fluorophores, photoprobes, inhibitors, enzymes, haptens, ligands, etc.). Bioconjugate Chemistry is intended to provide a forum for presentation of research relevant to all aspects of conjugation chemistry, including the preparation, characterization, and properties (both chemical and biological) of molecular conjugates. The journal emphasizes rigorous chemical standards and encourages application of modern techniques of chemical analysis to problems in conjugation chemistry. Every effort is made to process manuscripts quickly and efficiently. The time between acceptance and publication of Articles will be approximately 3 months (2 months for Letters). 3. Manuscript Classes. Comprehensive and critical accounts of significant studies should be submitted as Articles. The majority of publications will be in this category. Preliminary reports of sufficient contemporary importance and general interest to justify accelerated publication should be submitted as Letters. These are limited to 1000 words or the equivalent (additional supporting data may be requested even if space limitations prevent their publication). Reviews are short, interpretative accounts of subjects of active current interest. These are usually submitted at the invitation of the Editor, and preliminary communication with the Editor is advised before submitting an unsolicited review. Comments are brief discussions or criticisms of work previously published in the journal. These are subject to peer review, and the author is given an opportunity to reply. Teaching Editorials are brief introductions to a specific topic or area of current interest, written for the nonspecialist. Technical Notes are concise accounts of methods or techniques of wide applicability or interest. 4. Preparation of Manuscripts. (a) General Considerations. Manuscripts must be typed (or reproduced on a high-quality printer) doubled-spaced on 22 X 28 cm (or A4) paper on one side only. One copy must be a good, clear typescript, or photoduplicating machine product. The original manuscript should include the original inked drawings or photographs of structural formulas for direct use. High-quality output from laser printers is acceptable. General information on the preparation of manuscripts for ACS journals may be found in T h e ACS Style Guide (1986), available from the Sales Office, 1155 Sixteenth Street N.W., Washington, DC 20036. Authors should write in clear, concise English: the responsibility for all aspects of manuscript preparation rests with the authors.
Extensive changes or rewriting of the manuscript will not be undertaken by the editors. Articles should be assembled in the following order: title page (including full title, byline, and running title) and associated footnotes, abstract, introduction, experimental procedures (materials and methods), results, discussion, acknowledgments, references, tables, figure legends, and figures. All pages should be numbered consecutively starting with the title page. The length restriction on Letters precludes incorporation of an experimental procedure section and subdivisions within the text for introduction, results, and discussion. Essential experimental procedures should be incorporated into the text or included as footnotes. Abstracts of Letters are not published in the journal but are used by Chemical Abstracts, so an abstract must be submitted with each letter. Reviews and Teaching Editorials discuss published research and may employ a narrative style. Description of experimental procedures should be made throughout the text to an extent judged appropriate by the author. Comments and Technical Notes should be brief and to the point. (b) Title Page. A brief and informative title will aid in the classification and indexing of the paper. Do not use trade names of drugs or abbreviations. List full names and institutional affiliations of all authors and if differentiation is necessary indicate the affiliations of the authors by the superscript symbols t, $, 0, etc. The author to whom correspondence should be addressed is indicated by an asterisk. It is implicit in listing a person as an author that this individual has agreed to appear as an author of the manuscript. Provide a brief and informative running title that is easy to index and does not exceed 50 letters and spaces. (c) Abstract. The abstract should briefly present the problem and experimental approach and state the major findings and conclusions. It should be self-explanatory and suitable for reproduction without rewriting. Footnotes or undefined abbreviations may not be used. If a reference must be cited, complete publication data must be given. (d) Footnotes. Footnotes should be typed doublespaced and numbered in one consecutive series using supercript Arabic numerals. Author affiliations different from those stated on the title page can be listed as footnotes. In Letters, footnotes may be used to describe essential experimental details that are not easily incorporated in the text. Acknowledgment of financial support should not be listed in a footnote. Do not mix footnotes and reference citations. (e) Introduction. The introduction should state the purpose of the investigation and its relation to other work in the field. Background material should be brief and relevant to the research described. Detailed or lengthy reviews of the literature should be avoided. (f) Abbreviations. Standard abbreviations should be used throughout the manuscript. Note that abbreviations are used in ACS journals without periods. The preferred forms for some of the more commonly used abbreviations are mp, bp, "C, K, min, h, mL, &, g, mg, pg, cm, mm, nm, mol, mmol, pmol, ppm, HPLC, TLC, GC, NMR, GC-MS, and UV. All nonstandard abbreviations should be defined in a footnote following the first use of such an abbreviation in the text. (g) Experimental Procedures. The experimental procedures should be described in sufficient detail to en-
Bioconjugate Chem., Vol. 1, No. 2, 1990
able others to repeat the experiments. Names of products and manufacturers should be included only if alternate sources are deemed unsatisfactory. Novel experimental procedures should be described in detail. Care should be taken to describe any differences between published methods and methods actually used. Published procedures should merely be referred to by literature citation of both the original and any published modifications. Precautions for handling dangerous materials or for performing hazardous procedures should be stated or referenced. (h) Results. The results should be presented concisely. Tables and figures should be designed to maximize the presentation and comprehension of the experimental data. The same data should not be presented in more than one figure or in both a figure and a table. As a rule, interpretation of the results should be reserved for the discussion section of an Article, but under some circumstances (and in Letters) it may be desirable to combine results and discussion in a single section. In the interest of economy of space, it is sometimes desirable to place supplementary data (also subject to review) in a separate section for inclusion in the microfilm edition of the Journal. (i) Discussion. The purpose of the discussion is to interpret the results and to relate them to existing knowledge in the field in as clear and brief a fashion as possible. Information given elsewhere in the manuscript should not be repeated in the discussion. Extensive reviews of the literature should be avoided. (j) Acknowledgments. This section should acknowledge financial support, technical assistance, advice from colleagues, gifts, etc. Permission should be sought from persons whose contribution to the work is acknowledged in the manuscript. (k) References. References should clearly identify the original contributor to the work being cited. Textual references should be cited in one of the two following ways: (1)References may be cited by author and date. One author (Lohn, 1981); two authors (Carpenter & Cohen, 1979); three or more authors (Reboud et al., 1981). Citations in the reference list at the end of the manuscript should be arranged alphabetically, not numbered: One author, Wensel, T. G. (1984). Metal Chelates as Probes of Biological Systems. Acc. Chem. Res. 17, 202-209. Two authors, Wessels, B. W., and Rogus, R. D. (1984). Radionuclide Selection and Model Absorbed Dose Calculations for Radiolabeled Tumor Associated Antibodies. Med. Phys. 11, 638-640. Three or more authors, Moi, M. K., Meares, C. F., McCall, M. J., et al. (1985). Copper Chelates as Probes of Biological Systems: Stable Copper Complexes with a Macrocyclic Bifunctional Chelating Agent. Anal. Biochem. 148, 249-253. If there is more than one publication in a single year under the same authorship, use Williams et al. (1979a,b). (2) References may be numbered in one consecutive series in the text. Each literature reference should be assigned one number and placed in the text as an italicized Arabic numeral in parentheses. The complete list of references should be typed double-spaced beginning on a separate page after the acknowledgment and follow the format shown: (1)Fritzberg, A. R., Abrams, P. G., Beaumier, P. L., Kasina, s.,Morgan, A. C., Reno, J. M., Sanderson, J. A., Srinivasan, A., Wilbur, D. s.,and Vanderheyden, J. (1988) Specific and stable labeling of antibodies with technetium-99m with a diamide dithiolate chelating agent. Proc. Natl. Acad. Sci. U.S.A.85,4025-4029. Inclusive pagination is optional, and authors may elect to cite the first page of an article only. Titles of journals are abbreviated according to Chemical Abstracts Service Source Index. Serial publications such as Methods i n
163
Enzymology and C.R.C. Critical Reviews should be listed in the same form as journals. References to chapters and monographs are listed as follows: DeNardo, S. J., DeNardo, G. L., Deshpande, S. V., Adams, G. P., Macey, D. J., Mills, S. L., Epstein, A. L., and Meares, C. F. (1988) A Design of a Radiolabeled Monoclonal Antibody for Radioimmunodiagnosis and Radioimmunotherapy. Radiolabeled Monoclonal Antibodies for Imaging and Therapy (S. C. Srivastava, Ed.) pp 111-122, Plenum Publishing Corporation, New York. Submitted manuscripts should be designated as "in press" only if formally accepted for publication; otherwise "unpublished results" should be placed after the names of authors as a footnote in the text. (1) Spectral Data. It may be desirable to include such data for representative compounds in a series, for novel classes of compounds, and in structural determinations. Usually, it is not desirable to include routine spectral data for every compound in the manuscript. Papers where interpretations of spectra are critical to structural elucidation and those in which band shape or fine structure needs to be illustrated may be published with spectra included. When such presentations are deemed essential, only pertinent sections should be reproduced. (m)Biological Data. Biological test methods must be referenced or described in sufficient detail to permit the experiments to be repeated by others. Detailed descriptions of biological methods should be placed in the experimental procedures section. Data may be presented as numerical expressions or in graphical form. Statistical limits (statistical significance) for the biological data are usually required. If statistical limits cannot be provided, the number of determinations and some indication of the variability and reliability of the results should be given. References to statistical methods of calculation should be included. Doses and concentrations should be expressed as molar quantities (e.g., pmol/kg, mM, etc.) when comparisons of potencies are made on compounds having large differences in molecular weights. The routes of administration of test compounds and vehicles used should be indicated. (n)Tables. Tabulation of experimental results is encouraged when this leads to more effective presentation or to more economical use of space. Tables should be numbered consecutively with Roman numerals. Provide a brief title with each table and a brief heading for each column. Clearly indicate the units of measure (preferably SI). Data should be rounded to the nearest significant figure. Explanatory material referring to the whole table is to be included as a footnote to the title (a). Footnotes in tables should be given lower case letter designations and cited in the tables as italicized superscripts. Tables that require special treatment, such as insertion of arrows or other special symbols under or over alphanumeric characters, or contain many structures should be submitted as camera-ready copy. All tables should be cited in the text. ( 0 ) Figures. Figures should be submitted as original drawings or high-quality reproductions on single sheets of paper no larger than 22 X 28 cm. Figures may be printed with a 300-dpi laser printer on good quality white paper, preferably 25% rag bond. Photocopies can be used for duplicates. Lettering should be sufficiently large that it can be clearly read following reduction to single-column width (8.4 cm). The ideal reduced size for letters is 2 inm. For graphical presentation of data, symbols must be clearly discernible and brackets should be used to indicate the magnitude of statistical variation. Stereo diagrams should be submitted in single units with left and right images correctly positioned. Color reproduction is possible, but authors are charged for the incremental costs. (An esti-
164
Bioconjugate Chem., Vol. 1, No. 2, 1990
mate of these charges will be given upon request. A letter acknowledging the author’s willingness to defray the cost of color reproduction should accompany the revised manuscript.) Blocks of structural formulas should be designated as schemes or charts rather than figures. Figure legends (submitted on a separate page) should be sufficiently descriptive that the figure can be understood without reference to the text. The number of the figure and the name of the first author should be written on the back of each figure a t the top. (p) Structures. Structural formulas must be clearly drawn and should be constructed for maximum clarity in minimum space. Original drawings or high-quality reproductions should be grouped a t the end of the manuscript with the other illustrations. Structures with the same skeleton but containing different functional groups should be combined into a single formula with an appropriate legend to specify different chain lengths, substituents, etc. Line formulas should be used whenever possible. In the preparation of engraver’s copy of drawings, careful lettering (e.g., with a computer drawing program such as ChemDraw) is required. In preparing structural formulas for direct photoreproduction, authors should be mindful of the fact that equations, schemes, and blocks of structural formulas will be presented in the journal either in one column or two-column format (e.g., 8.5-cm or 17.7-cm width). It is recommended drawings be prepared at one of these two widths and submitted in the actual size that they will appear in the Journal. For greatest flexibility and page layout, the one-column format is preferred and should be used to the greatest extent possible. For authors using the ChemDraw program, the following preference items are recommended: fixed length, 18 point; line width, 1 point; bold width, 2.5 points. Single-width bold and dashed lines are preferred to wedges for stereochemical notation; 12-point Helvetica font should be used, both for atom labels and text materials. Drawings should be prepared with the page setup a t 60% and printed in this manner with a laser printer on good quality white paper, preferably 25% rag bond. (a) Nomenclature. It is the responsibility of the authors to provide correct nomenclature. All nomenclature must be consistent and unambiguous and should conform with current American usage. Insofar as possible, authors should use systematic names similar to those used by Chemical Abstracts Service, the International Union of Pure and Applied Chemistry, and the International Union of Biochemistry. CA nomenclature rules are contained in Appendix IV of the current Chemical Abstracts Index Guide. A list of ring systems, including names and numbering systems, is found in the Ring Systems Handbook, American Chemical Society, Columus, OH, 1988. Other recommendations include (1979) IUPAC Nomenclature of Organic Chemistry, 1978 ed., Pergamon Press, Elmsford,
NY; (1984) Enzyme Nomenclature, Academic Press, New York; (1978) Biochemical Nomenclature and Related Documents, The Biochemical Society, London. For CA nomenclature advice consult the Manager of Nomenclature Services, Department 64, Chemical Abstracts Service, P.O. Box 3012, Columbus, OH 43210. 5. Supplementary Material. Occasionally manuscripts are submitted with extensive tabular, graphical, or spectral data. Such “supplementary material” should be relegated to the microfilm edition of the journal. Authors are encouraged to use this resource in the interests of shorter articles which not only save journal space but also result in clearer and more readable presentations. However, “dumping“ of materials to this section is to be discouraged, and the Editors reserve the right to make downward adjustments in the numbers of such material presented. Materials to be placed in the microfilm edition should be separately identified with authors’ names and manuscript title and must be in a form easily handled for photoreproduction. Figure captions, titles to tables, and other identifying captions should appear on the same page as the figure or tables and not on a separate sheet. Preferable page size is 22 X 28 cm, with readable material aligned parallel with the 22 dimension if possible. Figures and illustrative material should be original drawings or black and white matte prints (not glossies) or originals. Typed sheets should be the originals, although good clear copies with clean characters and good contrast are acceptable. Type size or letter size should be large enough for easy reading. As a rule of thumb, if one has difficulty reading the material as submitted it is unacceptable. Refer to T h e ACS Style Guide for more specific information. A statement of the availability of supplementary data should be placed at the end of the Experimental Procedures section, using the following format: “Supplementary Material Available: Give description of material (x pages). Ordering information is given on any current masthead page.” 6. Proofs and Reprints. Manuscripts and proofs are sent to the author who submitted the papers. Proofs should be verified against the manuscript and appropriate corrections made. Substantial changes in a manuscript after type has been set require editorial approval and in some cases may be cause for re-reviewing. Changes are made at the author’s expense. An order form for reprints will be sent with the galley proofs by the printer; this form should be returned with the proof. 7. Corrections. If errors of consequence are found in the published paper, a correction of the error should be sent by the author to the Editor for publication in the “Additions and Corrections” section. 8. No Page Charges. There are NO page charges for Bioconjugate Chemistry.
