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Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses Chaohua Tang, Kai Zhang, Tengfei Zhan, Qingyu Zhao, and Junmin Zhang J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b03544 • Publication Date (Web): 30 Sep 2017 Downloaded from http://pubs.acs.org on October 1, 2017
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Journal of Agricultural and Food Chemistry
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Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood
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Biochemistry and Body Fluid Untargeted Metabolome Analyses
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Chaohua Tang§,¶,ǂ, Kai Zhang§,¶ ,ǂ, Tengfei Zhan§,¶, Qingyu Zhao§,¶, Junmin Zhang§,¶,*
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§
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Academy of Agricultural Sciences, Beijing 100193, China.
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¶
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Nutrition in North China, Ministry of Agriculture, Beijing, China.
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*
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86-10-62815890.
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State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese
Scientific Observing and Experiment Station of Animal Genetic Resources and
Corresponding author: email address:
[email protected], fax: 86-10-62815537, tel:
These two authors contributed equally to this work.
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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To characterize the metabolic disorders of dairy cows treated with gossypol, 12 dairy
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cows were assigned to either a control group or a treatment group that was fed 1000
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mg of gossypol per kilogram of dry matter feed for 28 days. Milk quality was
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adversely affected, as both milk protein and lactose levels were significantly
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decreased in the gossypol-treated group (3.40% vs. 3.16%, P = 0.044; 5.15% vs.
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4.91%, P = 0.027; respectively). Plasma samples revealed increases in alanine
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aminotransferase (P = 0.092), choline esterase (P = 0.02), and glutathione transferase
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(P = 0.0005), and decreases in glucose (P = 0.076) in the gossypol-treated group.
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Mass spectrometry-based comparative metabolomic analyses showed reduced
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concentrations of the gluconeogenesis precursor L-glutamine (P = 0.047), with
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significant decreases (P
