Revolutionize your LC m e a s u r e m e n t s with the amazing n e w Nicolet CHEMIGRAM™. T h e optical d e s i g n a n d c o m p u t a t i o n a l p o w e r of t h e Nicolet 7 1 9 9 FT-IR s y s t e m h a v e p r o d u c e d a p o w e r f u l , integrated LC-IR s y s t e m for routine analysis of e x t r e m e l y c o m p l e x LC and g e l permeation chromatography (GPC) samples. T h e FT-IR s y s t e m is u s e d a s a n "intelligent d e t e c t o r " with multiple benefits t o t h e user. Sample: 200 μg c y c l o h e x a n e (Peak 1 ) 200 μg tetrachloroethylene (Peak 2) 200 μg b e n z e n e (Peak 3) Conditions: 1 μΡΟΓββϋ* c o l u m n 0.5 m l / m i n C C 1 4 flow 0.2 m m KBr flowcell (1.5 μΙ) FT-IR Detector: H g C d T e detector (5000-300 c m 1 ) G e o n KBr beamsplitter 4 0 9 6 d a t a points (4 c m - ' resolution)
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Peak 1 and Peak 2
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Peak 3 benzene
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Differential detection of different substances is possible (30002800 cm-' window is sensitive only to cyclohexane, 3100-3010 cm- 1 and 690-650 c m ' windows are sensitive only to benzene, and 940890 cm-' window is sensitive only to tetrachloroethylene.) Complete chromatographic separation is not necessary for obtaining spectra of pure components (Peaks 1 and 2).
NICOLET. 7 1 9 9 F T - I R
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Benefits of t h e LC-IR C H E M I G R A M technique:
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Peak 2 tetrachloroethylene
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• A c t i v e cell v o l u m e s a s s m a l l a s 1.5 ul. • R e s o l u t i o n of 4 c m - ' o v e r r a n g e of 5 0 0 0 - 4 0 0 c m ' r o u t i n e l y a c h i e v e d . • F l o w c e l l operates at low pressure a n d requires n o special a l i g n m e n t s o it c a n b e e a s i l y c h a n g e d t o o p t i m i z e cell p a t h l e n g t h for p r e p a r a t i v e or analytical s a m p l e s . • Medium-strength solvent absorptions a r e completely r a t i o e d o u t s o t h e r e is n o s o l v e n t c o n t r i b u t i o n e x c e p t w h e r e s o l v e n t is c o m p l e t e l y o p a q u e . • N o p e a k s p r e a d i n g . • I n t e g r a t e s a b s o r b a n c e s in e a c h of u p t o f i v e o p e r a t o r s e l e c t e d IR b a n d s a n d p l o t s t h e m a g a i n s t e l u t e d v o l u m e ( a s C H E M I G R A M S ) in r e a l t i m e d u r i n g e a c h r u n . • I n j e c t i o n points a n d time a n d a b s o r b a n c e scales a r e automatically plotted. • W h e n a b s o r b a n c e e x c e e d s preselected t h r e s h o l d s c o m p l e t e s p e c t r a a r e s t o r e d o n d i s k a n d file n u m b e r s a r e i n d i c a t e d o n plot.
For m o r e details o n t h e world's finest FT-IR s y s t e m p l e a s e p h o n e or write Nicolet. 3Θ00
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1200
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Pure Peak 1 a n dPeak 2 spectra were
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NICOLET INSTRUMENT CORPORATION 5225 Verona Road Madison. Wisconsin 53711 Telephone: 608/271-3333
