25 Oct 2012 - The San Diego Center for Algae Biotechnology, Division of Biological Sciences, University of California San Diego, La Jolla,. California...
Oct 25, 2012 - Elizabeth A. Specht and Stephen P. Mayfield*. The San Diego Center for Algae Biotechnology, Division of Biological Sciences, University of ...
Oct 25, 2012 - Gene expression in chloroplasts is highly regulated during translation by sequence and secondary-structure elements in the 5â² untranslated ...
Aug 16, 2018 - Site saturation mutagenesis (SSM) is a powerful mutagenesis strategy for protein engineering and directed evolution experiments. However ...
Aug 16, 2018 - Site saturation mutagenesis (SSM) is a powerful mutagenesis strategy for protein engineering and directed evolution experiments. However ...
The mutants were classified with respect to gain of function (GOF) or loss of function (LOF) under unregulated or derepressed conditions using both a plate ...
Aug 16, 2018 - Application of this mutational approach further build the possibilities of synthetic biology in tuning of a response to known ligands and in ...
Nov 7, 1996 - Salt Lake City, Utah 84112, and NeXstar Pharmaceuticals, Inc., 2860 Wilderness Place,. Boulder, Colorado 80301. ReceiVed NoVember 7 ...
Nov 7, 1996 - Salt Lake City, Utah 84112, and NeXstar Pharmaceuticals, Inc., 2860 Wilderness Place,. Boulder, Colorado 80301. ReceiVed NoVember 7 ...
Abbreviated nomenclature for cyclic and branched homo- and hetero-detic peptides. J. Spengler , J.-C. Jimenez , K. Burger , E. Giralt , F. Albericio. Journal of ...
Matthew B. Francis, Nathaniel S. Finney, and Eric N. Jacobsen ... William Stegbauer , Frederick Streich , Rider Barnum , Eddie Chang , D. Andrew Knight.
SUPPORTING INFORMATION Table S1. List of all synthesized variants of the Chlamydomonas reinhardtii psbD 5’ untranslated region #
Table S2. List of all synthesized variants of the downstream 100 nucleotides of the Chlamydomonas reinhardtii psaA 5’ untranslated region # A001 A002 A003 A004 A005 A006 A007 A008 A009 A010 A011 A012 A013
Fwd for amplifying pGEM vector Rev for amplifying oligo library Fwd for amplifying psaA library Rev for amplifying pGEM for psaA Fwd for amplifying psbD library Rev for amplifying pGEM for psbD Fwd with barcode for amplifying psaA UTRs for NGS Fwd with barcode for amplifying psbD
UTRs for NGS Rev with barcode for amplifying original oligo library for NGS Rev with barcode for amplifying pGEM-vector library for NGS Rev with barcode for amplifying transformation vector library for NGS Rev with barcode for amplifying high expression lines for NGS Rev with barcode for amplifying medium expression lines for NGS Rev with barcode for amplifying low expression lines for NGS For constructing synthetic psaA UTR For constructing synthetic psaA UTR; also F primer for UTR amplification from individual clones of interest USER cloning of synthetic UTR; UTR fragment F USER cloning of synthetic UTR; UTR fragment R USER cloning of synthetic UTR; vector fragment 1 F USER cloning of synthetic UTR; vector fragment 1 R USER cloning of synthetic UTR; vector fragment 2 F USER cloning of synthetic UTR; vector fragment 2 R USER cloning of synthetic UTR; vector fragment 3 F USER cloning of synthetic UTR; vector fragment 3 R R primer for amplifying and sequencing individual UTRs of interest Amplifies control 16S gene in homoplasmic PCR screen Amplifies control 16S gene in homoplasmic PCR screen Amplifies gene integration locus in homoplasmic PCR screen Amplifies gene integration locus in homoplasmic PCR screen Fwd for qPCR of Lux amplicon Rev for qPCR of Lux amplicon Fwd for qPCR of chlB amplicon Rev for qPCR of chlB amplicon
