TΕCΗΝICΟΝ CHROMATOGRAPHY CORP. - Analytical Chemistry

May 29, 2012 - TΕCΗΝICΟΝ CHROMATOGRAPHY CORP. Anal. Chem. , 1964, 36 (13), pp 103A–103A. DOI: 10.1021/ac60219a814. Publication Date: ...
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in the assault1 on the protein molecule...

new automated technic provides rapid preparative and quantitative ... their size, occurrence and distribution., .and assures The Technicon AutoAnalyzer system you see above may well make history in protein research. By an ingenious extension of multiple-analysis chromatography, it not only separates peptides for preparative purposes, but at the same time gives quantitative information. The column effluent is split into many streams. One stream is subjected to a system of continuous hydrolysis. Another stream, unhydrolyzed, is used as control. Both are reacted with Ninhydrin. Com­ parison of these gives information about the size of the peptide. Other parallel streams can be used simultaneously for specific amino acid reactions.

information detection.

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peptides

Thus, the basic AutoAnalyzer® train can be aug­ mented and shaped at will to provide more and more analytic information. In doing this, the system takes up where paper chromatography, electrophoresis and UV monitor­ ing leave off. These methods are generally adequate enough for limited qualitative findings only. The Technicon® peptide system is automated from beginning to end with thoroughgoing AutoAnalyzer efficiency, rapidity, and reliability. This versatile AutoAnalyzer reduces to hours the days now con­ scripted to the tedious task of peptide analysis.

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Section of a chromatogrum showing tryptic hydrolyzate of performic acid oxidized bovine pancreatic Ribonuclease before and after alkaline hydrolysis.

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C H R O M A T O G R A P H Y CORP. R e s e a r c h P a r k · C h a u n c e y , Ν . Y. Technicon International of Canada, Ltd., Montreal; Technicon [nstrumenfs Company, Ltd., Londûn; Compagnie Technicon, Paris; Tec'inicon GmbH, Frankfurr/M.; Technicon AB, Stockholm; Technicon, Basle,- Technicon Italiana S.r.L., Rome; Technicon Equipment Pty., Ltd., Sydney; Compagnie Belge Technicon, Brussels,-Conitech A / S , Copenhagen Circle No. 152 on Readers' Service Card