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synthesized
F o r d e t e r m i n a t i o n of s e r u m albumin. ο - [ ( p -Hydroxyphenyl) azo ] benzoic Acid ( E A S T M A N 10614) (also known as H A B A ) H A B A d y e binds with serum albumin in phosphate buffer at p H 6.2 t o 6.4 t o form a colored complex with absorbance at 485nm. The reaction follows Beer's law over a wide range of concentrations. [Clin. Chim. Acta, 12,532 ( 1 9 6 5 ) ] .
For phosphorylation. 2-Cyanoethyl Phosphate Di(cyclohexylamine Salt) ( E A S T M A N 10964)
Monophosphate esters of nucleotides are readily pre pared b y using 2-cyanoethyl phosphate di(cyclohexylamine s a l t ) . F o r example, thymidine-3'-phosphate is prepared from 5'-0-tritylthymidine b y condensation, followed b y acid hydrolysis, then mild alkaline h y drolysis. [J.A.C.S., 83, 159 ( 1 9 6 1 ) ] . Similarly, steroid 21-phosphate esters are synthesized b y using 2-cyano ethyl phosphate. [J.A.C.S., 85, 1118 ( 1 9 6 3 ) ] .
F o r r e d u c t i o n of polycyclic quinones. Aluminum Cyclohexoxide ( 1 M in Cyclohexanol) ( E A S T M A N A10941)
Polycyclic quinones are converted t o hydrocarbons u n der reflux with aluminum cyclohexoxide; pentacene6,13-quinone yields pure pentacene. The starting quinone is readily prepared from phthalaldehyde a n d 1,4-cyclohexanedione. [Tetrahedron Letters, N o . 1, 5 (I960)].
For reduction, debromination, a n d s y n t h e s i s of c y c l o p r o p a n e s . Zinc-Copper Couple ( E A S T M A N 11122) Phthalide is prepared from phthalimide in 70% yield by using zinc-copper couple in aqueous alkali, followed by acidification and crystallization. [Org. Syn., Coll. Vol. 2, 526 ( 1 9 4 3 ) ] . 1,4-Dibromo-l-phenylpropane is debrominated with a mixture of zinc-copper couple and dimethylformamide t o yield cyclopropylbenzene. [Org. Syn., 44, 30 ( 1 9 6 4 ) ] . A number of cyclopropanes are prepared b y the S i m m o n s - S m i t h reaction of olefins and methylene iodide with zinc-copper couple. [/. Org. Chem., 24, 1825 (1959) a n d / . Am. Chem. Soc, 80, 5323 ( 1 9 5 8 ) ] .
in our laboratories,
one or more oi them could be the
We also offer some new reagents with only a few c o m ments. For synthesis of difluorocarbene : Lithium Trifluoracetate ( E A S T M A N 10953). For preparing t - B O C - a m i n o acids: tert-Butyl 2,4,5Trichlorophenyl Carbonate ( E A S T M A N 10914). For N-blocking of amino acids: Benzyl p-Nitrophenyl Carbonate ( E A S T M A N 10942). For cleaving N-protective phthaloyl groups in protein synthesis: Hydrazine Acetate ( E A S T M A N 11019). For selective formylation of terminal amine group of ornithine and lysine : p-Nitrophenyl Formate ( E A S T M A N 11008). For protection of hydroxyl and amine groups in s y n thesis of glycerides, steroids, a n d nucleosides: 2,2,2Trichloroethyl Chloroformate ( E A S T M A N 11050). For preparation of B h O C amino acids useful i n solidphase peptide synthesis: Benzhydryl Azidoformate ( E A S T M A N 11059).
G L C derivatizing reagent: Boron Fluoride ( 1 4 % in Propanol) ( E A S T M A N A10980). D y e laser, biological stain (C.I. # 4 5 1 6 0 ) , T L C visuali zation reagent: Rhodamine 6G ( E A S T M A N 10724). Titrant for non-aqueous titrimetry: Tetrabutylamm o n i u m Hydroxide Titrant [ 0.1 M in benzine-methanol ( 9 : 1 ) ] ( E A S T M A N A10934). For protein synthesis: Twenty N-blocked amino acids along with a number of other new reagents are listed in Kodak Publication JJ-161, Supple mental List of Reagents for Protein Synthesis and Structure Determination. U s e the coupon below to order your copy. All of these new chemicals are available from the E a s t m a n Organic Chemicals dealer nearest y o u . F o r a copy of the EASTMAN Organic Chemicals Catalog List 45, listing more than 6,000 reagents, and t o receive periodic supplements t o it automatically, s i m p l y c o m plete and return the coupon. B&A CURTIN FISHER HOWE & FRENCH
NORTH-STRONG PREISER SARGENT-WELCH WILL
Dept. 412L Eastman Organic Chemicals Eastman Kodak Company, Rochester, N.Y. 14650 Π Send JJ-1, "EASTMAN Organic Chemicals" catalog Π Send JJ-161, "Protein Reagents Supplement" Name
Kodak
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